食品科学 ›› 2021, Vol. 42 ›› Issue (24): 1-8.doi: 10.7506/spkx1002-6630-20210312-155

• 食品化学 •    

乳酸对玉米醇溶蛋白基面团品质提升的作用

刘昊,顾丰颖,朱金锦,杨婷婷,邵之晓,张巧真,王锋   

  1. (中国农业科学院农产品加工研究所,农业农村部农产品加工综合性重点实验室,北京 100193)
  • 发布日期:2021-12-30
  • 基金资助:
    国家自然科学基金面上项目(31871730);国家自然科学基金青年科学基金项目(31701642); 河南省重大公益专项(201300110200)

Effect of Lactic Acid on Improving the Quality of Zein-based Dough

LIU Hao, GU Fengying, ZHU Jinjin, YANG Tingting, SHAO Zhixiao, ZHANG Qiaozheng, WANG Feng   

  1. (Key Laboratory of Agro-products Processing, Ministry of Agriculture and Rural Affairs, Institute of Agro-products Processing Sciences and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China)
  • Published:2021-12-30

摘要: 以玉米醇溶蛋白、玉米淀粉、沙蒿胶为原料制备玉米醇溶蛋白基无麸质面团(简称Z面团),以玉米淀粉和沙蒿胶所制面团(简称NZ面团)为对照。通过调控面团中乳酸含量(L)和原料粉含量(F)的比例(简写为L/F,mL/g),比较乳酸含量对Z面团与NZ面团粉质性能的影响规律,表明玉米醇溶蛋白是影响Z面团粉质性能的关键组分。面团中L/F为2∶50的Z面团(Z2)稳定时间最长,弱化度最低,粉质质量指数最高,加工品质良好。探究在乳酸调节下,Z面团中玉米醇溶蛋白的理化性质与结构变化,发现乳酸会引起玉米醇溶蛋白的脱酰胺反应和蛋白质水解,促进α-玉米醇溶蛋白二倍体向单倍体转变,改变玉米醇溶蛋白的表面疏水性和二级结构组成。基于以上研究,将Z面团制为面条,考察乳酸含量对玉米醇溶蛋白基面条蒸煮品质的影响,其中Z2面条不易断条,蒸煮损失最少,质地良好。综上,适量的乳酸可改善玉米醇溶蛋白基无麸质面团粉质性能,提高玉米醇溶蛋白的表面疏水性,增加β-折叠占比,优化玉米醇溶蛋白基面条蒸煮品质。

关键词: 乳酸;玉米醇溶蛋白;粉质性能;脱酰胺反应;蒸煮品质

Abstract: In this study, zein-based gluten-free dough (Z dough) was prepared with zein, corn starch, and Artemisia sphaerocephala Krasch gum (ASKG). Also, zein-free dough (NZ dough) was prepared with corn starch and ASKG as control. The effects of adding different amounts of lactic acid solution on farinographic properties of the doughs were compared, indicating that zein crucially affects the farinographic properties of Z dough. Among the Z dough samples, Z2 with a ratio between lactic acid solution and the total amount of raw material (L/F) of 2:50 had the longest stability time, the lowest weakening degree, and the highest farinographic index. It was found that lactic acid could cause the deamidation and hydrolysis of zein in dough, promote the transformation of α-zein diploids to haploids, and change the surface hydrophobicity and secondary structure of zein. Further, Z dough was processed into noodles to investigate the influence of lactic acid content on the cooking quality of zein-based noodles. It was found that the noodles were not easy to break and had the lowest cooking loss and good texture. In summary, an appropriate amount of lactic acid can improve the farinographic properties of zein-based gluten-free dough, increase the surface hydrophobicity of zein and the proportion of β-sheet, and improve the cooking quality of zein-based noodles.

Key words: lactic acid; zein; farinographic properties; deamidation reaction; cooking quality

中图分类号: