食品科学 ›› 2022, Vol. 43 ›› Issue (9): 48-54.doi: 10.7506/spkx1002-6630-20210409-117

• 食品工程 • 上一篇    下一篇

基于静电纺丝法包封沙棘油纳米纤维的制备与表征

曹倩,王齐蕾,王枭,王艺璇,史婵,吕新刚   

  1. (西北大学食品科学与工程学院,陕西 西安 710069)
  • 出版日期:2022-05-15 发布日期:2022-05-27
  • 基金资助:
    国家自然科学基金青年科学基金项目(31401550)

Preparation and Characterization of Nanofibers Loaded with Sea Buckthorn Oil by Electrospinning Method

CAO Qian, WANG Qilei, WANG Xiao, WANG Yixuan, SHI Chan, LÜ Xingang   

  1. (College of Food Science and Technology, Northwest University, Xi’an 710069, China)
  • Online:2022-05-15 Published:2022-05-27

摘要: 通过静电纺丝技术制备负载沙棘油(sea buckthorn oil,SBO)的玉米醇溶蛋白(Zein)纳米纤维,并加入沙棘叶提取物(sea buckthorn leaf extract,SBE)以提升油脂稳定性和抗氧化性。在表征纳米纤维物理性状的基础上,分析其在加速氧化过程中色泽、过氧化值以及抗氧化活性的变化,并测定模拟消化过程中SBO的释放率和抗氧化活性。结果表明:Zein+SBO和Zein+SBO+SBE的包封率均大于90%;扫描电子显微镜观察结果显示制备的Zein+SBO+SBE纳米纤维形态均一;傅里叶变换红外光谱确认了SBO在纤维中成功包封且分散均匀;热重分析结果表明SBO纳米纤维在300 ℃左右开始降解,有较高的耐热性;添加SBE后,纳米纤维的抗氧化性大幅提升,SBO的氧化被明显抑制,纳米纤维包封与SBE添加协同提升了SBO的贮藏稳定性;纳米纤维包封可实现SBO在胃肠条件下的缓慢释放,且Zein+SBO+SBE在消化后仍保留了较高的抗氧化活性。结论:添加SBE与物理包封是提高SBO稳定性和抗氧化性的有效方法。

关键词: 沙棘油;沙棘叶;纳米纤维;静电纺丝;稳定性

Abstract: Zein nanofibers loaded with sea buckthorn oil (SBO) were prepared by electrospinning technology with the addition of sea buckthorn leaf extract (SBE) to improve oil stability and oxidation resistance. The physical properties of nanofibers were characterized, and the changes in the color parameters, peroxide value (PV) and antioxidant activity of encapsulated SBO were tested during accelerated oxidative storage. In addition, the changes in the release rate of SBO and antioxidant activity were also investigated during simulated digestion. The results showed that the encapsulation efficiency of zein + SBO and zein + SBO + SBE were both greater than 90%. Scanning electron microscopy (SEM) showed that the prepared nanofibers were uniform in shape, and Fourier transform infrared (FTIR) spectroscopy confirmed the successful encapsulation and uniform dispersion of SBO in the fiber. Thermogravimetric analysis (TGA) showed that SBO nanometer fibers began to degrade at around 300 ℃ and had high heat resistance. The addition of SBE greatly improved the oxidation resistance of nanofibers and inhibited the oxidation process of SBO, and significantly improved the storage stability of SBO in synergism with nanoencapsulation. Nano-encapsulated SBO was released slowly under gastrointestinal conditions, and the antioxidant activity of zein + SBO + SBE was still high after digestion. In conclusion, the addition of SBE and physical encapsulation are both effective in improving the stability and antioxidant activity of SBO.

Key words: sea buckthorn oil; sea buckthorn leaf; nanofibers; electrospinning; stability

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