• 生物工程 •

1. （哈尔滨商业大学 食品科学与工程重点实验室，黑龙江 哈尔滨 150076）
• 发布日期:2022-07-01
• 基金资助:
中央支持地方高校改革发展资金人才培养支持计划项目（高水平人才）（304017）； 黑龙江省自然科学基金联合引导项目（LH2020C063）；哈尔滨商业大学青年创新人才项目青年后备人才专项（2019CX33）

### Preparation of Flavin Adenine Dinucleotide Aptamer-Based Sensor and Its Application for Glucose Detection

WANG Yan, WANG Yue, ZHANG Shuai, ZHAO Ning, XIN Jiaying, SUN Lirui, GUAN Huanan

1. (Key Laboratory of Food Science and Engineering, Harbin University of Commerce, Harbin 150076, China)
• Published:2022-07-01

Abstract: An flavin adenine dinucleotide (FAD) aptamer-based sensor was constructed with gold nanoparticles (AuNPs) as the main body, and methanobactin (Mb) and FAD as the ligands. Mb was used to wrap AuNPs, which has a strong adsorption capacity for Mb, to form a stable Mb-AuNPs structure. The Mb-AuNPs structure was used to modify the surface of the bare gold electrode by the drip-coating method, and FAD was introduced into the Mb-AuNPs structure, in which the –COOH groups could firmly bind to FAD, to improve the ability to recognize glucose. As a result, a novel aptamer biosensor with glucose oxidase-like activity could be constructed. Cyclic voltammetry and the time-current curve method were used to explore the feasibility of its application in glucose detection. At 25 ℃ and pH 7.2, there was a good linear relationship between glucose concentration in the range of 10–200 mmol/L and the response current of the biosensor, with a correlation coefficient (R2) of 0.997 91, and the detection limit was 4.22 × 10-8 mol/L (RSN = 3). The aptamer-based sensor has several advantages such as simple preparation, low price, easy operation and rapid detection.