基于UPLC-QE-MS探究褐变反应对酸乳中代谢物的影响

doi:10.7506/spkx1002-6630-20210504-019

食品科学 ›› 2022, Vol. 43 ›› Issue (6): 272-278.doi: 10.7506/spkx1002-6630-20210504-019

基于UPLC-QE-MS探究褐变反应对酸乳中代谢物的影响

李萌，宁雪楠，康佳欣，廖敏和，任皓威，刘宁

（东北农业大学食品学院，乳品科学教育部重点实验室，黑龙江哈尔滨 150030）

出版日期:2022-03-25 发布日期:2022-03-28
基金资助:
“十三五”国家重点研发计划重点专项（2018YFC1604304）

Effect of Browning Reaction on the Metabolite Profile of Yogurt Analyzed by Untargeted Metabolomics Based on Ultra-high Performance Liquid Chromatography-Quadrupole Electrostatic Field Orbitrap Mass Spectrometry

LI Meng, NING Xuenan, KANG Jiaxin, LIAO Minhe, REN Haowei, LIU Ning

(Key Laboratory of Dairy Science, Ministry of Education, College of Food Science, Northeast Agricultural University, Harbin 150030, China)

Online:2022-03-25 Published:2022-03-28

摘要/Abstract

摘要： 采用超高效液相色谱-四极杆静电场轨道阱质谱非靶向代谢组学的方法，探究普通酸乳与褐色酸乳代谢物的差异，并从代谢物的角度评估2?种酸乳的不同。在正负离子模式下采集2?种酸乳的代谢物信息并结合多元统计分析方法对2?种酸乳的代谢物进行筛选，共得到117?种差异性代谢物。与普通酸乳相比，褐色酸乳中有106?种代谢物的相对含量上调，11?种代谢物的相对含量下调；褐色酸乳中有3?种风味氨基酸、8?种肽、12?种糖类、2?种美拉德反应产物以及苯醌等物质含量变化显著，本研究结果可为褐色酸乳的研究与开发提供一定理论依据。

关键词: 褐色酸乳；褐变反应；代谢组学；差异性代谢物

Abstract: In this study, untargeted metabolomics based on ultra-high performance liquid chromatography-quadrupole electrostatic field orbitrap mass spectrometry (UPLC-QE-MS) was used to explore the differences in metabolite profiles between ordinary yogurt and brown yogurt, and the differences between them were evaluated from the perspective of metabolites. The metabolite information of yogurts was collected in both the positive and negative ion modes, and the metabolites were screened by multivariate statistical analysis. A total of 117 differential metabolites were obtained. Compared with ordinary yogurt, the relative contents of 106 metabolites were up-regulated and 11 metabolites were down-regulated in brown yogurt. Compared with ordinary yogurt, the contents of 3 flavor amino acids, 8 peptides, 12 sugars, 2 Maillard reaction products and one quinone in brown yogurt changed significantly. The results of this study can provide some theoretical support for the future research and development of brown yogurt.

Key words: brown yogurt; browning reaction; metabonomics; differential metabolites

中图分类号:

TS252.54

李萌，宁雪楠，康佳欣，廖敏和，任皓威，刘宁. 基于UPLC-QE-MS探究褐变反应对酸乳中代谢物的影响[J]. 食品科学, 2022, 43(6): 272-278.

LI Meng, NING Xuenan, KANG Jiaxin, LIAO Minhe, REN Haowei, LIU Ning. Effect of Browning Reaction on the Metabolite Profile of Yogurt Analyzed by Untargeted Metabolomics Based on Ultra-high Performance Liquid Chromatography-Quadrupole Electrostatic Field Orbitrap Mass Spectrometry[J]. FOOD SCIENCE, 2022, 43(6): 272-278.

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基于UPLC-QE-MS探究褐变反应对酸乳中代谢物的影响

Effect of Browning Reaction on the Metabolite Profile of Yogurt Analyzed by Untargeted Metabolomics Based on Ultra-high Performance Liquid Chromatography-Quadrupole Electrostatic Field Orbitrap Mass Spectrometry

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