食品科学 ›› 2022, Vol. 43 ›› Issue (11): 113-120.doi: 10.7506/spkx1002-6630-20210505-027

• 营养卫生 • 上一篇    下一篇

3 种乳制品对低免疫力模型斑马鱼免疫功能的影响

史珅,李昊虬,王龙,李楠,谭圣杰,刘华,彭桂英,李亚兰,张冲,FENG HAOTIAN   

  1. (1.内蒙古乳业技术研究院有限责任公司,内蒙古 呼和浩特 010110;2.内蒙古伊利实业集团股份有限公司,内蒙古 呼和浩特 010110;3.北京中医药大学生命科学学院,北京 100029)
  • 出版日期:2022-06-15 发布日期:2022-06-30
  • 基金资助:
    “十三五”国家重点研发计划重点专项(2019YFF0301702);2020中央引领地方科技发展专项资金项目

Protective Effects of Three Types of Dairy Products on Immune Vulnerability in Vinorelbine-Treated Zebrafish

SHI Shen, LI Haoqiu, WANG Long, LI Nan, TAN Shengjie, LIU Hua, PENG Guiying, LI Yalan, ZHANG Chong, FENG HAOTIAN   

  1. (1. Inner Mongolia Dairy Technology Research Institute Co., Ltd., Hohhot 010110, China;2. Inner Mongolia Yili Industrial Group Co., Ltd., Hohhot 010110, China;3. School of Life Science, Beijing University of Chinese Medicine, Beijing 100029, China)
  • Online:2022-06-15 Published:2022-06-30

摘要: 目的:研究3 种乳制品对长春瑞滨(一种半合成的长春花生物碱)诱导的低免疫力斑马鱼免疫功能的调节作用。方法:分别给斑马鱼静脉注射0.2 mg/mL酒石酸长春瑞滨注射液诱导低免疫力模型,按照随机法将斑马鱼随机分为正常对照组、模型对照组、低温酸奶组(8 333 μg/mL)、常温纯牛奶组(70.4 μL/mL)、冷冻酸奶冰淇淋组(33 333 μg/mL),每组30 尾。利用荧光显微镜观察并分析3 种乳制品对低免疫力斑马鱼的巨噬细胞数量、巨噬细胞吞噬功能,以及T淋巴细胞数量的影响。结果:与正常对照组比较,模型对照组巨噬细胞数量显著降低、巨噬细胞吞噬功能减弱、T细胞数量高度显著降低(P<0.001)。与模型对照组比较,给予低温酸奶、常温纯牛奶和冷冻酸奶冰淇淋后巨噬细胞和T淋巴细胞的数量均显著增加(P<0.05、P<0.01和P<0.001);常温纯牛奶和冷冻酸奶冰淇淋均能显著增强巨噬细胞吞噬能力(P<0.05),低温酸奶对巨噬细胞吞噬功能的无显著促进作用。与低温酸奶比较,常温纯牛奶巨噬细胞数量显著提高(P<0.05),但T细胞数量和巨噬细胞吞噬功能无显著性差异(P>0.05);冷冻酸奶冰淇淋组巨噬细胞数量、T淋巴细胞数量、巨噬细胞吞噬功能均无显著性差异(P>0.05)。结论:低温酸奶、常温纯牛奶和冷冻酸奶冰淇淋可以通过增加巨噬细胞、T淋巴细胞的数量,改善巨噬细胞的吞噬能力,从而提高长春瑞滨诱发的低免疫力斑马鱼的免疫功能。

关键词: 乳制品;免疫功能;巨噬细胞;T细胞

Abstract: Objective: To examine the protective effects of three types of dairy products on immune vulnerability in vinorelbine-treated zebrafish. Methods: A zebrafish model of immunity vulnerability was established by intravenous injection of 0.2 mg/mL vinorelbine. The fish were randomly divided into five groups with 30 fish in each group: normal control, model control, low temperature yogurt (8 333 μg/mL), normal temperature pure milk (70.4 μL/mL), and frozen yogurt ice cream (33 333 μg/mL). The effects of the dairy products on the number of macrophages and T lymphocytes and macrophage phagocytosis in immunosuppressed zebrafish were evaluated using fluorescent microscopy. Results: Compared with the normal control group, the number of macrophages, the phagocytic function of macrophages and the number of T cells were significantly reduced in the model control group (P < 0.001). Compared with the model control group, the number of macrophages and T lymphocytes were significantly increased by treatment with each of the dairy products (P < 0.05, P < 0.01 or P < 0.001). Normal temperature pure milk and frozen yoghurt ice cream but not low temperature yoghurt could significantly enhance the phagocytosis capacity of macrophages (P < 0.05). Compared with low temperature yoghurt, normal temperature milk significantly increased the number of macrophages (P < 0.05), but resulted in no significant difference in the number of T cells or the phagocytic function of macrophages (P > 0.05); frozen yogurt ice cream also resulted in no significant difference in all of the parameters (P > 0.05). Conclusion: All these diary products can enhance the immune function of vinorelbine-treated zebrafish with immune vulnerability via increasing the number of macrophages and T lymphocytes and improving macrophage phagocytosis.

Key words: dairy products; immunity function; macrophages; T lymphocytes

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