魔芋葡甘露聚糖对高热能膳食诱导小鼠认知功能障碍及脑部氧化应激状态的影响

doi:10.7506/spkx1002-6630-20210813-167

食品科学 ›› 2022, Vol. 43 ›› Issue (15): 166-175.doi: 10.7506/spkx1002-6630-20210813-167

• 营养卫生 • 上一篇

魔芋葡甘露聚糖对高热能膳食诱导小鼠认知功能障碍及脑部氧化应激状态的影响

刘茜，李若雨，刘思宁，陈盼盼，方洁，汪浪红，龚桂萍，王仲孚，黄琳娟

（西北大学食品科学与工程学院，陕西省天然多糖资源利用工程研究中心，西安市糖生物学与糖工程重点实验室，陕西西安 710069）

发布日期:2022-08-30
基金资助:
西安市科学技术局农业技术研发项目（20NYYF0023）；陕西省教育厅一般专项（20JK0936）；中国博士后科学基金资助项目（2020M683542）；陕西省重点实验项目（16JS109）；陕西省大学生创新创业训练计划项目（S202010697172）

Effects of Konjac Glucomannan on Cognitive Dysfunction and Brain Oxidative Stress State in Mice Induced by a High-Calorie Diet

LIU Qian, LI Ruoyu, LIU Sining, CHEN Panpan, FANG Jie, WANG Langhong, GONG Guiping, WANG Zhongfu, HUANG Linjuan

(Shaanxi Natural Carbohydrate Resource Engineering Research Center, Xi’an Glycobiology and Glycoengineering Key Laboratory, College of Food Science and Technology, Northwest University, Xi’an 710069, China)

Published:2022-08-30

摘要/Abstract

摘要： 本研究旨在探讨不同分子质量（1、5、90 kDa）魔芋葡甘露聚糖（konjac glucomannan，KGM）对高脂高果糖膳食（high fat and high fructose diet，HFFD）诱导小鼠认知功能障碍及脑部氧化应激状态的干预作用。本实验通过HFFD造模，将90 只小鼠随机分为正常饮食组（ND）、阴性对照组（ND＋1 kDa KGM、ND＋5 kDa KGM、ND＋90 kDa KGM）、HFFD模型组（HFFD）、干预组（HFFD＋1 kDa KGM、HFFD＋5 kDa KGM、HFFD＋90 kDa KGM）和阳性对照组（HFFD＋KGM）。通过旷场、Y迷宫、水迷宫等行为学实验测试评估小鼠学习记忆能力，脑组织病理学切片观察神经细胞形态变化，测定脑组织谷胱甘肽（glutathione，GSH）相对含量和过氧化氢酶（catalase，CAT）相对活力，分析不同分子质量KGM对HFFD引起的小鼠认知功能紊乱及氧化应激状态的影响。结果显示，不同分子质量KGM均能够显著改善高热能膳食诱导小鼠的工作记忆能力（P＜0.05）；90 kDa KGM干预组小鼠在旷场及水迷宫定位巡航实验中的总移动距离显著增加（P＜0.05）；而1 kDa KGM干预组小鼠逃避潜伏期显著缩短（P＜0.05），且空间探索实验中小鼠穿越原平台的次数及在平台所在象限的移动距离显著增加（P＜0.05）。脑组织病理学切片结果显示，这些分子质量KGM对高热能膳食诱导小鼠海马区神经元的形态异常具有明显改善作用。抗氧化酶活力测定结果表明90 kDa KGM能显著地提高小鼠脑组织中GSH的相对含量（P＜0.05），1 kDa KGM能显著增加CAT相对活力（P＜0.05）。研究结果表明高分子质量（90 kDa）的KGM可以有效地提高小鼠自发活动能力，而低分子质量（1 kDa）的KGM可以更有效地改善高热能膳食小鼠的学习记忆能力，其机制可能与脑部氧化应激状态有关。

关键词: 魔芋葡甘露聚糖；分子质量；高热能膳食；认知功能障碍；氧化应激

Abstract: The purpose of this study was to explore the effects of konjac glucomannan (KGM) with different molecular masses (1, 5 and 90 kDa) on cognitive dysfunction and brain oxidative stress in mice induced by a high-calorie diet. In this study, 90 mice were randomly divided into nine groups: normal diet (ND), negative control (ND + 1 kDa KGM, ND + 5 kDa KGM, and ND + 90 kDa KGM), high-fat and high-fructose diet (HFFD) model, intervention (HFFD + 1 kDa KGM, HFFD + 5 kDa KGM, and HFFD + 90 kDa KGM), and positive control (HFFD + KGM). Behavioral tests including open field, Y maze and Morris water maze tests were conducted to evaluate the learning and memory ability of mice. Meanwhile, the morphological changes of brain nerve cells were evaluated by pathological section, and the relative content of glutathione (GSH) and the relative activity of catalase (CAT) in brain tissue were determined by commercial kits. The results demonstrated that KGM, regardless of its molecular mass, could significantly improve the spatial working memory capacity of mice fed HFFD (P < 0.05). The total movement distance of mice in the 90 kDa KGM intervention group was significantly increased in the open field and water maze positioning navigation tests (P < 0.05), while supplementation of 1 kDa KGM significantly decreased the escape latency (P < 0.05) and significantly increased the number of times that mice crossed the platform location and the movement distance in the quadrant (P < 0.05). Immunohistochemical results showed that konjac glucomannan markedly inhibited the morphological abnormalities of hippocampal neurons in the mouse brain induced by HFFD. Similarly, 90 kDa KGM significantly increased the relative level of GSH (P < 0.05), and 1 kDa KGM significantly up-regulated the relative activity of CAT in mouse brain tissue (P < 0.05). These results indicated that konjac glucomannan with high molecular mass (90 kDa) can effectively enhance the spontaneous activity of mice, while konjac glucomannan with low molecular mass (1 kDa) can significantly improve the learning and memory ability of mice fed a high-calorie diet. The underlying mechanism may be related to the oxidative stress state in the brain.

Key words: konjac glucomannan; molecular mass; high-calorie diet; cognitive dysfunction; oxidative stress

中图分类号:

TS201.4

刘茜，李若雨，刘思宁，陈盼盼，方洁，汪浪红，龚桂萍，王仲孚，黄琳娟. 魔芋葡甘露聚糖对高热能膳食诱导小鼠认知功能障碍及脑部氧化应激状态的影响[J]. 食品科学, 2022, 43(15): 166-175.

LIU Qian, LI Ruoyu, LIU Sining, CHEN Panpan, FANG Jie, WANG Langhong, GONG Guiping, WANG Zhongfu, HUANG Linjuan. Effects of Konjac Glucomannan on Cognitive Dysfunction and Brain Oxidative Stress State in Mice Induced by a High-Calorie Diet[J]. FOOD SCIENCE, 2022, 43(15): 166-175.

[1]	吴铭纪晨萱吴小勇郑传进何文江李文治尹辉. 天然活性产物抗电离辐射活性研究进展[J]. 食品科学, 0, (): 0-0.
[2]	丁俭杨梦竹黄祯秀黄永超马树林方勇. 食源蛋白水解物/多肽与糖类物质美拉德反应产物在食品应用研究中的进展[J]. 食品科学, 0, (): 0-0.
[3]	张弛郭静科胡雨嘉王旭峰刘树滔. 粗粮速食米替代高脂饮食对2型糖尿病小鼠糖脂代谢的影响[J]. 食品科学, 0, (): 0-0.
[4]	袁博卜伟戴威威鞠秀云王欣. 紫甘薯多糖分离、组分鉴定及抗氧化代谢机理[J]. 食品科学, 0, (): 0-0.
[5]	刘茜李若雨刘思宁陈盼盼方洁汪浪红龚桂萍王仲孚黄琳娟. KGM对高热能膳食诱导小鼠认知功能及脑部氧化应激状态的影响[J]. 食品科学, 0, (): 0-0.
[6]	张雪莉胡秋辉纪阳俞安淇仲磊赵立艳范育明. 富硒杏鲍菇蛋白的结构表征及其体外模拟消化产物对铅毒性的缓解作用[J]. 食品科学, 0, (): 0-0.
[7]	古佩娴尹惠双胡坤吴小勇钟南京郭娟黄超胡勇陈云王颖伍芳芳. 硒化猴头菇多糖的制备、结构表征及抗增殖活性[J]. 食品科学, 0, (): 0-0.
[8]	山波. 菌方普洱熟茶水提物和茶褐素的组成及其降脂作用[J]. 食品科学, 0, (): 0-0.
[9]	吴良文张虎吴桐陈宁. 白藜芦醇在阿兹海默症中的相关研究进展[J]. 食品科学, 0, (): 0-0.
[10]	刘辉周安妮胡胜男陈宁秦智贾绍辉. 小麦肽改善慢性疲劳大鼠认知障碍[J]. 食品科学, 0, (): 0-0.
[11]	胡美怡梁建兰李润丰杨晓宽. 一种新型板栗黄酒多糖的提取分离及其体外免疫活性[J]. 食品科学, 0, (): 0-0.
[12]	邓紫玙丁一侯欣尧李斌梁宏闪. 基于孢粉素微胶囊的共包埋、保护及递送益生菌和乳糖酶的体系[J]. 食品科学, 0, (): 0-0.
[13]	傅金凤谭思敏涂师运王娟盛鸥. 5种食品原料对高脂饮食诱导的肥胖大鼠肠道菌群和短链脂肪酸的影响[J]. 食品科学, 0, (): 0-0.
[14]	陈旎韩培培. 发菜藻粉对小鼠肠道菌群及免疫调节的影响[J]. 食品科学, 0, (): 0-0.
[15]	张馨方，杨亚兰，张慧，罗非君，任佳丽. 红汁乳菇中愈创木烷型倍半萜化合物的抗炎活性[J]. 食品科学, 2022, 43(13): 48-54.

魔芋葡甘露聚糖对高热能膳食诱导小鼠认知功能障碍及脑部氧化应激状态的影响

Effects of Konjac Glucomannan on Cognitive Dysfunction and Brain Oxidative Stress State in Mice Induced by a High-Calorie Diet

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价