乳杆菌耐消化应激能力及消化应激对其肠道黏附能力的影响

doi:10.7506/spkx1002-6630-20210830-383

食品科学 ›› 2022, Vol. 43 ›› Issue (14): 143-150.doi: 10.7506/spkx1002-6630-20210830-383

• 生物工程 • 上一篇

乳杆菌耐消化应激能力及消化应激对其肠道黏附能力的影响

陈大卫，程月，任晨瑜，陈春萌，瞿恒贤，瓦云超，燕宪涛，陈霞，黄玉军，张臣臣，关成冉，郑英明，顾瑞霞

（1.扬州大学食品科学与工程学院，江苏省乳品生物技术与安全控制重点实验室，江苏扬州 225127；2.江苏宇航食品科技有限公司，江苏盐城 224000）

发布日期:2022-07-28
基金资助:
江苏省自然科学基金面上项目（BK20211325）；江苏省高等学校自然科学研究重大项目（19KJA140004）；国家自然科学基金面上项目（31972094）；“十三五”国家重点研发计划重点专项（2019YFF0217602）；江苏省科技计划项目-苏北科技专项（XZ-SZ202042）；市校合作共建科技创新平台项目（YZ2020265）；江苏省乳业生物工程技术中心开放课题（ZK2018002）

Capability of Lactobacillus to Tolerate Digestive Stress and Effect of Digestive Stress on Its Intestinal Adhesion Capacity

CHEN Dawei, CHENG Yue, REN Chenyu, CHEN Chunmeng, QU Hengxian, WA Yunchao, YAN Xiantao,CHEN Xia, HUANG Yujun, ZHANG Chenchen, GUAN Chengran, ZHENG Yingming, GU Ruixia

(1. Jiangsu Province Key Lab of Dairy Biotechnology and Safety Control, College of Food Science and Engineering, Yangzhou University, Yangzhou 225127, China; 2. Jiangsu Yuhang Food Technology Co. Ltd., Yancheng 224000, China)

Published:2022-07-28

摘要/Abstract

摘要： 首先通过体外模拟唾液-胃液-肠液应激实验研究乳杆菌的耐消化应激能力，然后研究其对肠道黏蛋白和Caco-2细胞的黏附及抑制肠道病原菌黏附的能力，最后探讨消化应激对乳杆菌黏附能力的影响。结果表明，副干酪乳杆菌W125、m111和发酵乳杆菌146在依次经过模拟唾液-胃液-肠液应激后存活率分别为2.70%、3.53%及11.15%，活菌数分别为7.46、7.24（lg（CFU/mL））及 8.35（lg（CFU/mL）），且对黏蛋白和Caco-2细胞的黏附率显著高于其他菌株（P＜0.05），分别为15.67%、8.75%、8.38%和11.47%、21.34%、10.44%；3 株菌株均可通过排除、竞争和替代的方式抑制大肠杆菌CICC10899和沙门菌WX29对肠道的黏附，黏附抑制率均大于13.51%；消化应激显著降低了副干酪乳杆菌W125和发酵乳杆菌146对肠道的黏附能力（P＜0.05），但显著增加了副干酪乳杆菌m111的黏附能力（P＜0.05），黏附率由17.60%增加到30.45%，且主要黏附素由消化应激前的表层蛋白变为应激后的蛋白和多糖；消化应激前后副干酪乳杆菌m111均为长梭状、圆润的杆状，表层物质的厚度也没有发生显著变化（P＞0.05）。副干酪乳杆菌m111具有较强的耐消化应激以及对肠道黏附的能力，且消化应激可提高其黏附肠道的能力，是1 株潜在的具有良好应用价值的益生乳杆菌。

关键词: 乳杆菌；消化应激；黏附能力

Abstract: The aim of this study was to evaluate the tolerance of Lactobacillus to digestive stress in simulated saliva, gastric juice and intestinal juice, its adhesion capacity to intestinal mucin and Caco-2 cells and its inhibitory capacity against intestinal pathogen adhesion. The effect of digestive stress on the intestinal adhesion capacity of Lactobacillus was also studied. The results showed that the viable counts of L. paracasei?m111 and W125 and L. fermentum 146 were 7.46, 7.24 and 8.35 (lg(CFU/mL)), respectively, and their survival rates were 2.70%, 3.53% and 11.15%, respectively, after sequential exposure to simulated saliva, gastric juice and intestinal juice. Their adhesion rates to mucin were 15.67%, 8.75% and 8.38%, and their adhesion rates to Caco-2 cells were 11.47%, 21.34%, 10.44%, respectively, which were significantly higher than those of the other strains (P < 0.05). Each of the three strains had the capability to inhibit the intestinal adhesion capacity of Escherichia coli CICC10899 and Salmonella WX29 by exclusion, competition or substitution, with an inhibition percentage of more than 13.51%. Digestive stress significantly decreased the intestinal adhesion capacity of L. paracasei W125 and L. fermentum 146 (P < 0.05), but increased the intestinal adhesion capacity of L. paracasei m111 from 17.60% to 30.45% (P < 0.05). After digestive stress, the major adhesins changed from surface proteins to proteins and polysaccharides, the morphology of L. paracasei m111 remained long spindle-like, round rod-like in shape, whereas the thickness of the surface material was not significant changed (P > 0.05). In conclusion, L. paracasei m111 has strong tolerance to digestive stress and consequently strong intestinal adhesion capacity, making it a potential probiotic with a promising future.

Key words: Lactobacillus; digestive stress; adhesion ability

中图分类号:

TS252.54

陈大卫，程月，任晨瑜，陈春萌，瞿恒贤，瓦云超，燕宪涛，陈霞，黄玉军，张臣臣，关成冉，郑英明，顾瑞霞. 乳杆菌耐消化应激能力及消化应激对其肠道黏附能力的影响[J]. 食品科学, 2022, 43(14): 143-150.

CHEN Dawei, CHENG Yue, REN Chenyu, CHEN Chunmeng, QU Hengxian, WA Yunchao, YAN Xiantao,CHEN Xia, HUANG Yujun, ZHANG Chenchen, GUAN Chengran, ZHENG Yingming, GU Ruixia. Capability of Lactobacillus to Tolerate Digestive Stress and Effect of Digestive Stress on Its Intestinal Adhesion Capacity[J]. FOOD SCIENCE, 2022, 43(14): 143-150.

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乳杆菌耐消化应激能力及消化应激对其肠道黏附能力的影响

Capability of Lactobacillus to Tolerate Digestive Stress and Effect of Digestive Stress on Its Intestinal Adhesion Capacity

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