三甲胺柱[5]芳烃的合成及其抑菌性能

doi:10.7506/spkx1002-6630-20211116-201

食品科学 ›› 2023, Vol. 44 ›› Issue (1): 46-52.doi: 10.7506/spkx1002-6630-20211116-201

三甲胺柱[5]芳烃的合成及其抑菌性能

吴惠香，杨华，赵登奇，黄建颖，杨利军

（浙江工商大学食品与生物工程学院，浙江省食品安全重点实验室，浙江杭州 310018）

出版日期:2023-01-15 发布日期:2023-01-31
基金资助:
浙江省自然科学基金项目（LY20B040001）；浙江省基础公益技术研究计划农村农业项目（LGN19C200008）

Synthesis and Antibacterial Properties of Trimethylamine-Based Cationic Pillar[5]arene

WU Huixiang, YANG Hua, ZHAO Dengqi, HUANG Jianying, YANG Lijun

(Food Safety Key Laboratory of Zhejiang Province, School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310018, China)

Online:2023-01-15 Published:2023-01-31

摘要/Abstract

摘要： 本研究合成基于三甲胺的柱[5]芳烃，并以金黄色葡萄球菌（Staphylococcus aureus ATCC 6538）和大肠杆菌（Escherichia coli DH5α）为供试菌对三甲胺柱[5]芳烃的抑菌性能和抑菌机制进行分析。通过抑菌实验确定三甲胺柱[5]芳烃最小抑菌浓度（minimal inhibit concentration，MIC）和最小杀菌浓度（minimal bactericidal concentration，MBC），并探讨其对细菌生物被膜形成的影响；利用透射电子显微镜（transmission electron microscopy，TEM）观察三甲胺柱[5]芳烃对细菌细胞膜的损伤情况，并通过噻唑蓝（3-(4,5-dimethyl-2-thiazyl)-2,5-diphenyl-2H-tetrazolium bromide，MTT）法测定其细胞毒性。结果显示，三甲胺柱[5]芳烃对S. aureus ATCC 6538的MIC和MBC分别为0.125 mg/mL和1.000 mg/mL，对E. coli DH5α的MIC和MBC分别为0.250 mg/mL和＞1.000 mg/mL；三甲胺柱[5]芳烃对S. aureus ATCC 6538生物被膜形成的抑制效果优于对E. coli DH5α。TEM观察结果表明三甲胺柱[5]芳烃对不同菌株细胞膜的损伤不同；MTT实验结果表明MIC范围内三甲胺柱[5]芳烃无毒。本研究结果明确了三甲胺柱[5]芳烃的具体抑菌性能，可为其在食品领域的进一步开发利用提供一定的理论依据。

关键词: 三甲胺柱[5]芳烃；抑菌性能；细胞膜损伤；细胞毒性

Abstract: In this study, a pillar[5]arene was synthesized based on trimethylamine, and its antibacterial activity and mechanism against Staphylococcus aureus ATCC 6538 and Escherichia coli DH5α were investigated. The minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) were determined, and the influence of the pillar[5]arene on the formation of bacterial biofilms was evaluated. Transmission electron microscopy (TEM) was used to observe the damage to the bacterial cell membrane caused by trimethylamine-based pillar[5]arene, and the cytotoxicity was determined by the?3-(4,5-dimethyl-2-thiazyl)-2,5-diphenyl-2H-tetrazolium?bromide (MTT) method. The results showed that the MIC and MBC were 0.125 and 1.000 mg/mL for S. aureus ATCC 6538, respectively, and 0.250 and greater than 1.000 mg/mL for E. coli DH5α, respectively. The effect of trimethylamine-based pillar[5]arene on biofilm formation was more pronounced for S. aureus ATCC 6538 than for E. coli DH5α. TEM showed that the trimethylamine-based pillar[5]arene could differently damage the cell membrane of the two strains. The MTT assay showed that the trimethylamine-based pillar[5]arene was non-toxic within the MIC range. The results of this study provide a theoretical basis for further development and utilization of the trimethylamine-based pillar[5]arene in the food field.

Key words: trimethylamine-based pillar[5]arene; antibacterial activity; membrane damage; cytotoxicity

中图分类号:

TS201.3

吴惠香，杨华，赵登奇，黄建颖，杨利军. 三甲胺柱[5]芳烃的合成及其抑菌性能[J]. 食品科学, 2023, 44(1): 46-52.

WU Huixiang, YANG Hua, ZHAO Dengqi, HUANG Jianying, YANG Lijun. Synthesis and Antibacterial Properties of Trimethylamine-Based Cationic Pillar[5]arene[J]. FOOD SCIENCE, 2023, 44(1): 46-52.

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三甲胺柱[5]芳烃的合成及其抑菌性能

Synthesis and Antibacterial Properties of Trimethylamine-Based Cationic Pillar[5]arene

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