长期发酵大头菜差异代谢物及其关联代谢通路分析

doi:10.7506/spkx1002-6630-20211220-236

食品科学 ›› 2022, Vol. 43 ›› Issue (22): 192-198.doi: 10.7506/spkx1002-6630-20211220-236

• 生物工程 • 上一篇

长期发酵大头菜差异代谢物及其关联代谢通路分析

张钰麟，陈泓帆，赵志平，王卫，聂鑫，程杰，张崟，刘达玉，徐跃成，罗淮良

（1.成都大学肉类加工四川省重点实验室，四川成都 610106；2.成都大学食品与生物工程学院，四川成都 610106；3.成都医学院基础医学院，四川成都 610500；4.四川省食品检验研究院，四川成都 610100；5.自贡市泰福农副产品加工厂，四川自贡 643101）

发布日期:2022-12-12
基金资助:
固态发酵资源利用四川省重点实验室开放基金项目（2019GTY004）；四川省科技计划项目（2020YFN0148）

Analysis of Differential Metabolites and Related Metabolism Pathways in Long-Term Fermented Kohlrabi Fermented for Different Periods

ZHANG Yulin, CHEN Hongfan, ZHAO Zhiping, WANG Wei, NIE Xin, CHENG Jie, ZHANG Yin, LIU Dayu, XU Yuecheng, LUO Huailiang

(1. Meat Processing Key Laboratory of Sichuan Province, Chengdu University, Chengdu 610106, China; 2. College of Food and Biological Engineering, Chengdu University, Chengdu 610106, China; 3. School of Basic Medical Science, Chengdu Medical College, Chengdu 610500, China; 4. Sichuan Institute of Food Inspection, Chengdu 610100, China; 5. Zigong Taifu Agricultural and Sideline Products Processing Plant, Zigong 643101, China)

Published:2022-12-12

摘要/Abstract

摘要： 为探究工业化生产不同超长周期发酵大头菜代谢物的差异及其主要关联代谢通路，采用液相色谱-串联质谱技术结合多元统计分析，测定并比较0、5、10 a大头菜的代谢物。结果表明，发酵0 a与5 a、发酵5 a与10 a、发酵0 a与10 a分别鉴定出53、113 种和174 种差异代谢物（变量投影重要性值＞1.3，P＜0.05），主要为有机酸、氨基酸及核酸及其衍生物等。3 个处理组共同的差异代谢物有19 种，包含L-苯丙氨酸、β-D-果糖-6-磷酸等。差异代谢物京都基因与基因组百科全书通路富集分析显示，苯丙氨酸代谢、磷酸戊糖途径、果糖和甘露糖代谢途径与主要差异代谢物相关性最显著。本研究揭示了不同超长周期发酵大头菜代谢物的差异和主要差异代谢物及其关联代谢通路，为定向调控大头菜特征代谢物的生物合成提供一定理论依据。

关键词: 大头菜；长期发酵；代谢组学；液相色谱-串联质谱；差异代谢物

Abstract: In order to explore the differences in the metabolite profiles of industrially long-term fermented kohlrabi with different fermentation periods and the major metabolic pathways involved, the metabolite profiles of kohlrabi fermented for 0, 5 and 10 years were analyzed and compared by using liquid chromatography-tandem mass spectrometry (LC-MS/MS) combined with multivariate statistical analysis. The results showed that a total of 53, 113 and 174 differential metabolites were identified in years 0 versus 5, 5 versus 10, and 0 versus 10 of fermentation, respectively (variable importance in the projection (VIP) > 1.3, P < 0.05), including organic acids, amino acids, nucleic acids and their derivatives. Nineteen differential metabolites were common to the three fermentation time points, including L-phenylalanine, and β-D-fructose-6-phosphate. The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis of the differential metabolites showed that phenylalanine metabolism, the pentose phosphate pathway, and the fructose and mannose metabolic pathways were the most significantly correlated with the major differential metabolites. The present study provides a theoretical basis for targeted regulation of the biosynthesis of the characteristic metabolites in fermented kohlrabi.

Key words: kohlrabi; long-term fermentation; metabolomics; liquid chromatography-tandem mass spectrometry; differential metabolites

中图分类号:

TS255.53

张钰麟，陈泓帆，赵志平，王卫，聂鑫，程杰，张崟，刘达玉，徐跃成，罗淮良. 长期发酵大头菜差异代谢物及其关联代谢通路分析[J]. 食品科学, 2022, 43(22): 192-198.

ZHANG Yulin, CHEN Hongfan, ZHAO Zhiping, WANG Wei, NIE Xin, CHENG Jie, ZHANG Yin, LIU Dayu, XU Yuecheng, LUO Huailiang. Analysis of Differential Metabolites and Related Metabolism Pathways in Long-Term Fermented Kohlrabi Fermented for Different Periods[J]. FOOD SCIENCE, 2022, 43(22): 192-198.

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长期发酵大头菜差异代谢物及其关联代谢通路分析

Analysis of Differential Metabolites and Related Metabolism Pathways in Long-Term Fermented Kohlrabi Fermented for Different Periods

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