食品科学

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纯种植物乳杆菌发酵低盐萝卜泡菜的研究

汪立平1,2,汪 欣1,艾连中2,吴正钧2,赵 勇1   

  1. 1.上海海洋大学 上海水产品加工及贮藏工程技术研究中心,上海 201306;
    2.光明乳业股份有限公司 乳业生物技术国家重点实验室,上海 600597
  • 出版日期:2013-09-15 发布日期:2013-09-27
  • 通讯作者: 汪立平
  • 基金资助:

    上海市科委工程中心建设项目(11DZ2280300);
    光明乳业股份有限公司乳业生物技术国家重点实验室开放基金项目(SKLDB2011-005)

Fermentation of Low-salt Pickled Radish by Pure Strains of Lactobacillus plantarum

WANG Li-ping1,2,WANG Xin1,AI Lian-zhong2,WU Zheng-jun2,ZHAO Yong1     

  1. 1. Shanghai Engineering Research Center of Aquatic Product Processing and Preservation, Shanghai Ocean University, Shanghai 201306, China;
    2. State Key Laboratory of Dairy Biotechnology, Bright Dairy and Food Co. Ltd., Shanghai 600597, China
  • Online:2013-09-15 Published:2013-09-27
  • Contact: WANG Li-ping

摘要:

利用从传统泡菜中筛选到的4株植物乳杆菌纯种发酵萝卜泡菜,以商业菌株B110和自然发酵泡菜为对照,以得到合适的泡菜发酵剂。结果表明:接种植物乳杆菌发酵泡菜有缩短发酵时间、降低亚硝酸含量等优点,其中植物乳杆菌STW-4表现最佳;采用单因素试验确定STW-4发酵的最佳条件为食盐质量浓度8g/100mL、接种量2%,室温发酵,发酵周期从自然发酵的7d缩短到60h。为了降低接种泡菜的含盐量同时保留萝卜的脆度,将萝卜原料经过10g/100mL高质量浓度食盐预处理24h后,在4g/100mL低食盐质量浓度条件下再接种植物乳杆菌STW-4,得到成品泡菜的脆度为22.98N,相对不经过高盐预处理组的15.67N能够明显提高脆度,这可能跟高盐预处理增加了萝卜失水程度及对果胶酶和纤维素酶活性的破坏有关。

关键词: 植物乳杆菌, 低盐, 萝卜泡菜, 脆度

Abstract:

In order to find an appropriate starter for the fermentation of pickled radish, four pure strains of
Lactobacillus plantarum isolated from traditionally pickled radish were used for the fermentation of fresh radish and
compared with Lactobacillus planterum B110 and naturally fermented pickle. The results indicated that Lactobacillus
plantarum STW-4 was the best culture starter due to the shorter fermentation time and lower nitrite content. The
fermentation conditions were optimized and as a result, the fermentation cycle was reduced to 60 h from 7 days when
compared with naturally fermented pickle under the conditions of 2% inoculation, room temperature and 8 g/100 mL salt.
In order to reduce the salt concentration but not destroy the brittleness, Lactobacillus plantarum STW-4 was inoculated
in the presence of 4 g/100 mL salt, and the brittleness of pickled turnips was 22.98 N, which revealed an improvement
from 15.67 N. This improvement may be related to the increased water loss and the inhibited activities of pectinase and
cellulose under a high salt concentration.

Key words: Lactobacillus plantarum, low-salt, pickled radish, brittleness

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