甘露聚糖酶AfMan5A和RmMan134C的性质差异分析及对多糖的协同降解

doi:10.7506/spkx1002-6630-20220105-030

食品科学 ›› 2022, Vol. 43 ›› Issue (24): 145-153.doi: 10.7506/spkx1002-6630-20220105-030

• 生物工程 • 上一篇

甘露聚糖酶AfMan5A和RmMan134C的性质差异分析及对多糖的协同降解

刘学，卢海强，王玉印，田洪涛，谷新晰

（河北农业大学食品科技学院，河北保定 071000）

发布日期:2022-12-28
基金资助:
国家自然科学基金青年科学基金项目（31901631）

Differences in Enzymatic Properties between Mannanases from Aspergillus fumigatus (AfMan5A) and Rhizopus microsporus (RmMan134C) and Their Synergy in the Degradation of Polysaccharides

LIU Xue, LU Haiqiang, WANG Yuyin, TIAN Hongtao, GU Xinxi

(College of Food Science and Technology, Hebei Agricultural University, Baoding 071000, China)

Published:2022-12-28

摘要/Abstract

摘要： 分析烟曲霉（Aspergillus fumigatus）HBHF5来源的GH5家族甘露聚糖酶AfMan5A和微孢根霉（Rhizopus microsporus）GZHF10来源的GH134家族甘露聚糖酶RmMan134C的酶学性质差异，探究两者协同降解多糖的潜力。结果表明两者酶学性质差异较大，AfMan5A的最适温度和pH值分别为60 ℃和6.0，而RmMan134C最适温度和pH值为35 ℃和5.0。金属离子Cu2＋、Zn2＋和Mn2＋均表现出对2 个酶活性的抑制，而Mg2＋、Fe3＋和Li＋可促进AfMan5A活性，却对RmMan134C活性表现出轻微抑制。甘露聚糖酶RmMan134C较AfMan5A底物谱广，两者最适底物均为魔芋葡甘露聚糖。进一步研究发现，两者并未对甘露聚糖类底物表现出协同效果，而是在微晶纤维素的降解中表现出较好的协同作用，协同系数为6.1。本研究分析了GH5和GH134家族甘露聚糖酶的性质差异及降解多糖的协同效果，为甘露聚糖酶的进一步应用推广提供一定的理论支持。

关键词: GH5家族；GH134家族；β-甘露聚糖酶；酶学性质；协同

Abstract: This study aimed to analyze the differences in the enzymatic properties of mannanases from Aspergillus fumigatus HBHF5 (AfMan5A, belonging to the GH5 family) and Rhizopus microsporus GZHF10 (RmMan134C, belonging to the GH134 family), and to explore their potential to synergistically degrade polysaccharides when used in combination. The results showed that the enzymatic properties of AfMan5A and RmMan134C were quite different. The optimum temperature and pH of AfMan5A were 60 ℃ and 6.0, respectively, while those of RmMan134C were 35 ℃ and 5.0, respectively. Cu2+, Zn2+, Mn2+ showed inhibitory activity against both enzymes, while Mg2+, Fe3+ and Li+ could promote the activity of AfMan5A, but slightly inhibit the activity of RmMan134C. RmMan134C exhibited a broader spectrum of substrates than AfMan5A, and the best substrate for both of them was konjac glucomannan. Furthermore, they did not synergistically act on mannan-based substrates but showed a good synergistic effect in the degradation of microcrystalline cellulose, with a synergy coefficient of 6.1. This study analyzed the difference in properties of GH5 and GH134 family mannanases and the synergistic effect of degrading polysaccharides, and provided a certain theoretical support for the further application and promotion of mannanases.

Key words: GH5 family; GH134 family; β-mannanase; enzymatic properties; synergy

中图分类号:

Q814

刘学，卢海强，王玉印，田洪涛，谷新晰. 甘露聚糖酶AfMan5A和RmMan134C的性质差异分析及对多糖的协同降解[J]. 食品科学, 2022, 43(24): 145-153.

LIU Xue, LU Haiqiang, WANG Yuyin, TIAN Hongtao, GU Xinxi. Differences in Enzymatic Properties between Mannanases from Aspergillus fumigatus (AfMan5A) and Rhizopus microsporus (RmMan134C) and Their Synergy in the Degradation of Polysaccharides[J]. FOOD SCIENCE, 2022, 43(24): 145-153.

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甘露聚糖酶AfMan5A和RmMan134C的性质差异分析及对多糖的协同降解

Differences in Enzymatic Properties between Mannanases from Aspergillus fumigatus (AfMan5A) and Rhizopus microsporus (RmMan134C) and Their Synergy in the Degradation of Polysaccharides

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