食品科学 ›› 2023, Vol. 44 ›› Issue (1): 22-29.doi: 10.7506/spkx1002-6630-20220209-032

• 基础研究 • 上一篇    下一篇

硒硫互作对白菜芽苗菜硫代葡萄糖苷含量及抗氧化性的影响

赵爽,周羽琪,杨旭妍,范震宇,李方全,袁太勇,吴桐,王玉书   

  1. (齐齐哈尔大学生命科学与农林学院,黑龙江 齐齐哈尔 161006)
  • 出版日期:2023-01-15 发布日期:2023-01-31
  • 基金资助:
    国家自然科学基金青年科学基金项目(31401908);黑龙江省自然科学基金项目(C2016056); 黑龙江省省属高等学校基本科研业务费项目(135509221;135409217;135509219); 黑龙江省普通高等学校青年创新人才培养计划项目(UNPYSCT-2017155)

Effect of Selenium and Sulfur Interaction on the Glucosinolate Content and Antioxidant Activity of Sprouts of Chinese Cabbage

ZHAO Shuang, ZHOU Yuqi, YANG Xuyan, FAN Zhenyu, LI Fangquan, YUAN Taiyong, WU Tong, WANG Yushu   

  1. (College of Life Science and Agriculture Forestry, Qiqihar University, Qiqihar 161006, China)
  • Online:2023-01-15 Published:2023-01-31

摘要: 为探讨硒硫互作对白菜芽苗菜硫代葡萄糖苷(以下简称硫苷)含量及抗氧化性的影响,以白菜种子为实验材料,通过4 mmol/L ZnSO4溶液单独喷施、50 μmol/L和100 μmol/L的Na2SeO3溶液单独喷施以及二者联合喷施,分析白菜芽苗菜主要生理生化指标、抗氧化能力及硫苷含量等变化规律。结果表明,单独施用ZnSO4溶液对白菜芽苗菜造成了生长胁迫,而单独施用Na2SeO3溶液可增加芽长和单株平均质量;Na2SeO3溶液联合ZnSO4溶液喷施处理可以有效缓解单独施用ZnSO4溶液对白菜芽苗菜生长发育的抑制作用。同时,相较于对照(喷施去离子水),经过ZnSO4溶液联合Na2SeO3溶液喷施处理后,白菜芽苗菜硒元素含量及总硫苷含量均显著提高(P<0.05),其中ZnSO4溶液联合50 μmol/L Na2SeO3溶液喷施处理6 d后总硫苷含量增加约50%;总体上发芽6 d的芽苗菜硒元素和硫苷含量明显高于4 d时的芽苗菜。研究结果可为生产富硒高硫苷的功能性白菜芽苗菜提供参考。

关键词: 白菜芽苗菜;亚硒酸钠;硫酸锌;硫代葡萄糖苷;抗氧化能力

Abstract: In order to explore the effect of selenium-sulfur interaction on the glucosinolate content and antioxidant properties of Chinese cabbage sprouts, the seeds were sprayed with 4 mmol/L ZnSO4, 50 or 100 μmol/L Na2SeO3 or their combination during sprouting and the changes in the major physiological and biochemical indexes, antioxidant capacity and glucosinolates content of Chinese cabbage sprouts were analyzed. The results showed that the application of ZnSO4 alone caused growth stress on Chinese cabbage sprouts, while the application of Na2SeO3 alone increase the length and fresh mass of sprouts per plant. The combined application of Na2SeO3 and ZnSO4 could effectively alleviate the inhibitory effect of ZnSO4 on the growth and development of Chinese cabbage sprouts. Moreover, compared with the control group (deionized water), ZnSO4 combined with Na2SeO3 significantly increased total selenium content and the contents of total glucosinolates (P < 0.05), and the combined application of ZnSO4 and 50 μmol/L Na2SeO3 resulted in an approximate 50% increase in the total content of glucosinolates. The contents of total selenium and total glucosinolates were significantly higher on the sixth day than on the fourth day of sprouting. The results of the study provide data support for the production of functional Chinese cabbage sprouts rich in selenium and glucosinolates.

Key words: Chinese cabbage sprouts; Na2SeO3; ZnSO4; glucosinolates; antioxidant activity

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