茭白壳提取物对小鼠溃疡性结肠炎的预防作用

doi:10.7506/spkx1002-6630-20220602-027

食品科学 ›› 2022, Vol. 43 ›› Issue (23): 97-105.doi: 10.7506/spkx1002-6630-20220602-027

茭白壳提取物对小鼠溃疡性结肠炎的预防作用

严媛，吴伟杰，郜海燕，房祥军，韩延超，刘瑞玲，牛犇，陈杭君

（1.南京农业大学食品科学技术学院，江苏南京 210008；2.浙江省农业科学院食品科学研究所，农业农村部果品采后处理重点实验室，农业农村部蔬菜采后保鲜与加工重点实验室（部省共建），浙江省果蔬保鲜与加工技术研究重点实验室，中国轻工业果蔬保鲜与加工重点实验室，浙江杭州 310021）

出版日期:2022-12-15 发布日期:2022-12-28
基金资助:
国家自然科学基金面上项目（32072285）；农业农村部现代农业产业技术体系建设专项（CARS-24-E-01）

Preventive Effect of Zizania latifolia Shell Extract on Ulcerative Colitis in Mice

YAN Yuan, WU Weijie, GAO Haiyan, FANG Xiangjun, HAN Yanchao, LIU Ruiling, NIU Ben, CHEN Hangjun

(1. College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210008, China; 2. Key Laboratory of Post-harvest Handling of Fruits, Ministry of Agriculture and Rural Affairs, Key Laboratory of Postharvest Preservation and Processing of Vegetables (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Key Laboratory of Fruits and Vegetables Postharvest and Processing Technology Research of Zhejiang Province, Key Laboratory of Postharvest Preservation and Processing of Fruits and Vegetables, China National Light Industry, Institute of Food Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China)

Online:2022-12-15 Published:2022-12-28

摘要/Abstract

摘要： 本研究通过乙醇浸提、硅胶色谱层析得到茭白壳提取物（Zizania latifolia shell extract，Zlse），探究其对小鼠溃疡性结肠炎的影响，并通过网络药理学对Zlse预防小鼠溃疡性结肠炎的机制进行初步分析。实验设置正常组、模型组、阳性对照组和Zlse低、中、高剂量处理组（Zlse-L组、Zlse-M组、Zlse-H组），模型组、阳性对照组、Zlse组通过灌胃3.5%（质量分数）葡聚糖硫酸钠溶液建立溃疡性结肠炎模型，阳性对照组和Zlse组分别进行美莎拉嗪和Zlse灌胃处理，正常组和模型组给予0.5%（质量分数）羧甲基纤维素钠溶液灌胃，每天记录小鼠健康情况，进行疾病活动指数（disease active index，DAI）评分，比较6 组小鼠体质量变化率、DAI评分、结肠长度、苏木精-伊红染色切片、组织病理学评分、炎症因子（白细胞介素（interleukin，IL）-6、IL-8、IL-1β、肿瘤坏死因子-α（tumor necrosis factor-α，TNF-α））水平、髓过氧化物酶（myeloperoxidase，MPO）活力、超氧化物歧化酶（superoxide dismutase，SOD）活力；通过GeneCards、OMIM、TTD和DAVID数据库筛选关键靶点及重要京都基因与基因组百科全书（Kyoto Encyclopedia of Genes and Genomes，KEGG）通路，构建“活性成分-靶点-KEGG通路”网络图。结果表明，相较于模型组，Zlse组体质量降低趋势显著减缓（P＜0.05），各组结肠长度有所恢复，结肠损伤减轻，此外，Zlse组血清中各促炎症因子水平显著降低（P＜0.05），结肠组织MPO活力显著降低（P＜0.05），SOD活力显著升高（P＜0.05）；通过网络药理学分析获得8 个活性成分、77 个交集靶点和119 条KEGG通路，关键活性成分为小麦黄素及小麦黄素-4’-O-丁香醇醚，其中重要通路包括磷脂酰肌醇3-激酶/苏氨酸激酶信号通路、受体激活和活性氧积累等，与动物实验结果相印证。结果表明Zlse能够通过抑制促炎症因子的释放，调控结肠抗氧化防御能力，预防葡聚糖硫酸钠盐诱导的小鼠溃疡性结肠炎，且其功效与浓度呈正相关。

关键词: 茭白壳；网络药理学；小麦黄素；溃疡性结肠炎

Abstract: In this study, Zizania latifolia shell extract (Zlse) was obtained by ethanol extraction and silica gel chromatography, and its effect on ulcerative colitis in mice was evaluated. The underlying mechanism was analyzed by network pharmacology. Six groups of mice were established: normal control (NC), model control (MC), positive control (PC), low-dose Zlse (Zlse-L), medium-dose Zlse (Zlse-M) and high-dose Zlse (Zlse-H). Ulcerative colitis was induced by intragastric administration of 3.5% sodium dextran sulfate solution in the MC, PC and Zlse groups. The PC and Zlse groups were intragastrically administrated with mesalazine and Zlse, respectively. The NC and MC groups were intragastrically administrated with 0.5% sodium carboxymethylcellulose (CMC-Na) solution. The health status of mice was recorded daily, and the disease activity index (DAI) score was calculated. The percentage body mass change, DAI score, colon length, hematoxylin and eosin (HE)-stained colonic sections, colonic histopathology score, serum inflammatory factors (IL-6, IL-8, IL-1β, and TNF-α) levels, and the activities of colonic myeloperoxidase (MPO) and superoxide dismutase (SOD) were compared between the six groups. Based on the key targets and the important Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways obtained from the GeneCards, OMIM, TTD and DAVID databases, a ‘active ingredient-target-KEGG pathway’ network diagram was constructed. The results showed that compared with the MC group, the trend of body mass loss in the Zlse treatment groups was significantly slowed down (P < 0.05), the colon length was restored, and the colon injury was reduced. In addition, the levels of serum proinflammatory factors in the Zlse groups were significantly decreased (P < 0.05); colonic MPO activity was significantly decreased (P < 0.05), while SOD activity was increased (P < 0.05). Eight active components, 77 intersection targets and 119 KEGG pathways were obtained by network pharmacology analysis. The key active components were tricin and tricin-4’-O-syringyl alcohol, and the important pathways included the P13K/Akt signaling pathway, receptor activation and reactive oxygen species accumulation, which were confirmed by animal experiments. The results showed that Zlse can concentration-dependently prevent DSS-induced ulcerative colitis in mice by inhibiting the release of proinflammatory factors and regulating the antioxidant defense capacity of the colon.

Key words: Zizania latifolia shell; network pharmacology; tricin; ulcerative colitis

中图分类号:

TS201.2

严媛，吴伟杰，郜海燕，房祥军，韩延超，刘瑞玲，牛犇，陈杭君. 茭白壳提取物对小鼠溃疡性结肠炎的预防作用[J]. 食品科学, 2022, 43(23): 97-105.

YAN Yuan, WU Weijie, GAO Haiyan, FANG Xiangjun, HAN Yanchao, LIU Ruiling, NIU Ben, CHEN Hangjun. Preventive Effect of Zizania latifolia Shell Extract on Ulcerative Colitis in Mice[J]. FOOD SCIENCE, 2022, 43(23): 97-105.

[1]	唐文潇肖瀛蒋天宁周一鸣. 烘焙度对冷萃咖啡理化指标与风味成分的影响[J]. 食品科学, 2022, 43(24): 0-0.
[2]	王司琪刘学军王佳佳李泊铮. 提取方法对玉木耳膳食纤维结构特征和功能特性的影响[J]. 食品科学, 2022, 43(24): 0-0.
[3]	杨玉洁刘焕王淑惠柳春红陈树喜周爱梅. 基于酶活力和细胞模型分析广佛手多糖降血糖活性及作用机制[J]. 食品科学, 0, (): 0-0.
[4]	张晶晶，李艾黎，程金菊. 美拉德反应修饰的酪蛋白递送生物活性物质的应用研究进展[J]. 食品科学, 2022, 43(23): 316-325.
[5]	陈贵婷，胡坦，徐阳，徐港，田蕾，吴思宇，戴易祎，潘思轶. 柑橘膳食纤维理化性质、生理功能及其在食品中的应用研究进展[J]. 食品科学, 2022, 43(23): 336-345.
[6]	张晶晶李艾黎程金菊. 美拉德反应修饰的酪蛋白递送生物活性物质的应用研究进展[J]. 食品科学, 0, (): 0-0.
[7]	陈贵婷胡坦徐阳徐港田蕾吴思宇戴易祎潘思轶. 柑橘膳食纤维理化性质、生理功能及其在食品中的应用[J]. 食品科学, 0, (): 0-0.
[8]	李祥雨熊雅婷滕建文韦保耀黄丽夏宁. 基于气相色谱-质谱技术与多元统计分析对咸蛋黄热加工中异味组分分析[J]. 食品科学, 0, (): 0-0.
[9]	钟玉旺徐万莉范尧珠黎依艳王雪峰. 辣木籽ACE抑制肽的分离纯化、结构鉴定及其体外活性评价[J]. 食品科学, 0, (): 0-0.
[10]	张俊林陈祉晴郑钦生肖杰曹庸刘晓娟. 基于分子对接和表面等离子共振技术研究虾青素与跨膜转运蛋白CD36的结合作用[J]. 食品科学, 0, (): 0-0.
[11]	王玲玉闫春梅贾梦王安琪汪轩羽王新涛李秀荷王慧芳戴福宏李宁周中凯高铁成. 油莎豆膳食纤维对短链脂肪酸生产能力和肠道菌群分布特征的影响[J]. 食品科学, 0, (): 0-0.
[12]	李思涵王君巧聂少平. 沙苑子多糖的提取纯化与结构解析[J]. 食品科学, 0, (): 0-0.
[13]	吴旋李娜徐怀德李梅. 晚期糖基化终末产物形成及抑制机理的研究进展[J]. 食品科学, 0, (): 0-0.
[14]	杨婷婷郝宗围任李顺陈光未黄艾祥. 玉米醇溶蛋白负载植物甾醇纳米颗粒的制备及性能[J]. 食品科学, 0, (): 0-0.
[15]	钟泽梁洪碧红肖美添白锴凯. 多糖纳米硒的制备、功能和在食品领域应用的研究进展[J]. 食品科学, 0, (): 0-0.

茭白壳提取物对小鼠溃疡性结肠炎的预防作用

Preventive Effect of Zizania latifolia Shell Extract on Ulcerative Colitis in Mice

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价