食品科学 ›› 2023, Vol. 44 ›› Issue (17): 29-35.doi: 10.7506/spkx1002-6630-20220802-021

• 基础研究 • 上一篇    下一篇

山苍子精油对白假丝酵母菌细胞膜屏障影响的机理

孙月,曾朝懿,李梓钰,熊海波,林籽汐,车振明,唐洁   

  1. (西华大学食品与生物工程学院,四川 成都 610039)
  • 出版日期:2023-09-15 发布日期:2023-09-29
  • 基金资助:
    四川省科技厅关键技术攻关项目(2019YFN0173);四川省大学生创新创业训练计划项目(S201910623053); 西华大学食品生物技术省高校重点实验室资助项目(SZjj2017-114)

Mechanism for the Effect of Litsea cubeba Essential Oil on Candida albicans Cell Membrane Barrier

SUN Yue, ZENG Chaoyi, LI Ziyu, XIONG Haibo, LIN Zixi, CHE Zhenming, TANG Jie   

  1. (College of Food and Bioengineering, Xihua University, Chengdu 610039, China)
  • Online:2023-09-15 Published:2023-09-29

摘要: 为研究山苍子精油对白假丝酵母菌的抑菌活性及其作用机理,本实验对山苍子精油主要成分进行分析,用不同质量浓度的山苍子精油处理筛选自“生花”泡菜中的白假丝酵母菌,观察处理前后白假丝酵母菌细胞形态、表面疏水性、表面电荷、胞内核酸和蛋白质泄漏变化情况。结果表明,山苍子精油主要成分有柠檬醛、柠檬烯、香茅醛。山苍子精油对白假丝酵母菌的最低抑菌浓度为0.25 mg/mL,最低杀菌浓度为1 mg/mL。在YPD培养基中加入山苍子精油进行处理后,白假丝酵母菌细胞生长延滞,细胞膜完整性受损,通透性增加,表明山苍子精油具有良好的透膜能力,可以破坏细胞结构,使细胞膜通透性紊乱,胞内物质渗漏,从而导致细胞死亡。本研究可为将山苍子精油应用于抑制发酵蔬菜汁和奶制品贮藏期间白假丝酵母菌生长提供理论依据。

关键词: 白假丝酵母菌;山苍子精油;主要成分;抑菌活性;抑菌机理

Abstract: This study was carried out in order to clarify the antifungal activity and mechanism of the essential oil of Litsea cubeba against Candida albicans. The major components of the essential oil were analyzed, and changes in the cell morphology, surface hydrophobicity, surface charge, intracellular nucleic acid and protein leakage of Candida albicans isolated from contaminated pickles were evaluated after being treated with the essential oil. The results showed that the major components of Litsea cubeba essential oil were citral, limonene, and citronellal. The minimum inhibitory concentration (MIC) was 0.25 mg/mL, and the minimum bactericidal concentration (MBC) was 1 mg/mL. The addition of Litsea cubeba essential oil to yeast extract peptone dextrose (YPD) medium delayed cell growth, impaired cell membrane integrity, and increased cell membrane permeability, indicating that Litsea cubeba essential oil has good membrane permeability, and can disrupt cell structure, disorder cell membrane permeability, and result in leakage of intracellular materials, thus leading to cell death. This study provides a theoretical basis for the application of Litsea cubeba essential oil to inhibit the growth of Candida albicans during the storage of fermented vegetable juice and dairy products.

Key words: Candida albicans; Litsea cubeba essential oil; main components; antifungal activity; antifungal mechanism

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