食品科学 ›› 2023, Vol. 44 ›› Issue (12): 306-314.doi: 10.7506/spkx1002-6630-20220901-019

• 成分分析 • 上一篇    下一篇

基于GC-IMS和GC-MS分析不同发酵方式对黄桃酒香气成分的影响

马宁原,姚凌云,孙敏,王化田,宋诗清,常煦,冯涛   

  1. (1.上海应用技术大学香料香精技术与工程学院,上海 201418;2.安琪酵母股份有限公司,湖北 宜昌 443003)
  • 出版日期:2023-06-25 发布日期:2023-06-30

Effect of Different Fermentation Methods on Aroma Composition of Yellow Peach Wine Analyzed by Gas Chromatography-Mass Spectrometry and Gas Chromatography-Ion Mobility Spectrometry

MA Ningyuan, YAO Lingyun, SUN Min, WANG Huatian, SONG Shiqing, CHANG Xu, FENG Tao   

  1. (1. School of Perfume and Aroma Technology, Shanghai Institute of Technology, Shanghai 201418, China; 2. Angel Yeast Co. Ltd., Yichang 443003, China)
  • Online:2023-06-25 Published:2023-06-30

摘要: 研究巴斯德毕赤酵母(Pichia pastoris)与商业酿酒酵母BV818(Saccharomyces cerevisiae)不同接种方式和自然发酵对黄桃酒风味的影响。以奉贤黄桃为原料,除了自然发酵(自然发酵组)以外,将巴斯德毕赤酵母与酿酒酵母BV818分别同时接种(同时接种组)、间隔1 d顺序接种发酵(混菌发酵1组)、间隔3 d顺序接种发酵(混菌发酵2组)、单独接种酿酒酵母(BV818发酵组),通过气相色谱-质谱(gas chromatography-mass spectrometry,GC-MS)联用和气相-离子迁移谱(gas chromatography-ion mobility spectrometry,GC-IMS)结合感官特性(定量描述分析)研究不同发酵方式酿造对黄桃酒中挥发性风味物质的影响。结果表明:所有乙醇发酵均在15 d内完成,通过GC-MS和GC-IMS分别检测到31 种和22 种化合物。混菌发酵组增加了醇类,减少了酸类、酯类物质含量,但增加了萜烯类、醇类、酸类物质种类。感官评价中,混菌发酵组比其他香气丰富度和香气平衡度更高。同时发酵坚果和甜香得分更高,自然发酵的类酸香最强。本研究表明混菌发酵可以提高黄桃酒的香气,毕赤酵母与酿酒酵母混菌发酵对黄桃酒酿造有应用价值,其中混菌发酵2组的感官评分最高。

关键词: 黄桃酒;气相色谱-质谱联用;气相-离子迁移谱;挥发性化合物;巴斯德毕赤酵母;感官评价;偏最小二乘回归

Abstract: The objective of this study was to investigate the effects of natural fermentation and inoculated fermentations with Pichia pastoris and active dry wine yeast BV818 (Saccharomyces cerevisiae) on the flavor of yellow peach wine. Simultaneous inoculation, sequential inoculation of P. pastoris and BV818 with a time interval of one and three days, and inoculation of BV818 alone were used. Gas chromatography-mass spectrometry (GC-MS), gas chromatography-ion mobility spectrometry (GC-IMS) and quantitative descriptive sensory analysis were used to study the effects of different fermentation methods on volatile flavor substances in yellow peach wine. The results showed that for all groups, ethanol fermentation was completed within 15 days, and 31 and 22 compounds were detected by GC-MS and GC-IMS, respectively. In the sequential fermentation groups, the content of alcohols increased, the contents of acids and esters decreased, and the numbers of terpenes, alcohols and acids increased. In the sensory evaluation, the sequential fermentation groups had higher aroma richness and aroma balance than the other groups. The simultaneous fermentation group scored higher in sensory score for nutty and sweet aroma. The naturally fermented wine had the strongest sour aroma. In summary, mixed fermentation with P. pastoris and S. cerevisiae can improve the aroma of yellow peach wine, and the sensory score of the wine produced by sequential inoculation with a three-day interval was highest.

Key words: yellow peach wine; gas chromatography-mass spectrometry; gas chromatography-ion mobility spectrometry; volatile organic compounds; Pichia pastoris; sensory evaluation; partial least squares regression

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