食品科学 ›› 2023, Vol. 44 ›› Issue (19): 27-34.doi: 10.7506/spkx1002-6630-20221018-174

• 食品工程 • 上一篇    

超声功率对牛肉干品质及牛肉肌原纤维蛋白结构的影响

董智铭,么紫瑶,姜萩婉,蒋博文,王见钊,陈倩,张文涛   

  1. (东北农业大学食品学院,黑龙江 哈尔滨 150030)
  • 发布日期:2023-11-07
  • 基金资助:
    国家自然科学基金面上项目(31972139);黑龙江省“百千万”工程重大科技专项(2020ZX07B02;2021ZX12B05)

Effect of Ultrasonic Power on the Quality and Myofibrillar Protein Structure of Beef Jerky

DONG Zhiming, YAO Ziyao, JIANG Qiuwan, JIANG Bowen, WANG Jianzhao, CHEN Qian, ZHANG Wentao   

  1. (College of Food Science, Northeast Agricultural University, Harbin 150030, China)
  • Published:2023-11-07

摘要: 为改善传统牛肉干质地坚硬、咀嚼性差、品质不稳定等问题,本实验采用超声辅助腌制技术处理原料肉,研究不同超声功率(0、150、300、450 W和600 W,时间30 min,频率30 kHz)辅助腌制处理后的新鲜牛肉条和经6 d自然发酵的牛肉干中水分质量分数、水分活度、pH值、色泽、氯化钠质量分数(以下简称盐含量)、剪切力等品质特性,以及超声处理后新鲜牛肉条中肌纤维碎片化指数、表面疏水性、羰基含量、总巯基含量和活性巯基含量等的变化情况,并对牛肉干进行感官评价,探究超声功率对牛肉干品质特性及牛肉肌原纤维蛋白结构的影响。结果表明,牛肉干中水分质量分数、水分活度、pH值、盐含量、L*值和b*值均随着超声功率的提高逐渐增加,而剪切力逐渐降低。超声处理有利于牛肉干a*值增加,但功率过大会降低a*值。随着超声功率的提高,肌原纤维蛋白的肌纤维碎片化程度、羰基含量、活性巯基含量和表面疏水性均呈现上升趋势,而总巯基含量呈现显著下降趋势(P<0.05)。感官评价结果表明,超声功率300 W的牛肉干整体可接受性最佳。综上,为了保持颜色稳定性,避免蛋白过度氧化以及能源浪费,确定超声功率300 W为最佳处理条件,此条件下的超声处理可明显提高牛肉干的整体品质。

关键词: 牛肉干;超声辅助腌制;超声功率;品质;蛋白结构

Abstract: In order to improve the hard texture, poor chewiness and unstable quality of traditional beef jerky, the effect of of ultrasonic power on the quality and myofibrillar protein structure of beef jerky, prepared by ultrasonic-assisted marination at different ultrasonic powers (0, 150, 300, 450 and 600 W) and 30 kHz frequency for 30 min followed by natural fermentation for six days, was explored. The quality characteristics of marinated beef strips and beef jerky such as moisture content, water activity, pH, color, salt content and shear force were evaluated. Meanwhile, changes in the myofibrillar fragmentation index (MFI), surface hydrophobicity, carbonyl group content, and total sulfhydryl and active sulfhydryl group contents of myofibrillar proteins in fresh beef strips after ultrasonic treatment were investigated and sensory evaluation of beef jerky were carried out. Results showed that the pH, moisture content, water activity, salt content, L* value and b* value of beef jerky increased with the increase in ultrasonic power, while the shear force decreased. Ultrasonic treatment increase the a* value, whereas the opposite effect was observed at excessively high ultrasonic power. With the increase in ultrasonic power, the MFI, surface hydrophobicity, active sulfhydryl group content and carbonyl group content of myofibrillar proteins increased, while the total sulfhydryl group content decreased (P < 0.05). Sensory evaluation showed that beef jerky with ultrasonic treatment at 300 W had the best overall acceptability. In summary, to maintain color stability and avoid excessive oxidation of protein and energy waste, an ultrasonic power of 300 W was determined as the optimal treatment condition, which could significantly improve the overall quality of beef jerky.

Key words: beef jerky; ultrasonic-assisted curing; ultrasonic power; quality; protein structure

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