大鲵肽联合跑台运动对肥胖大鼠的减重效果及机制

doi:10.7506/spkx1002-6630-20221124-288

食品科学 ›› 2024, Vol. 45 ›› Issue (5): 69-77.doi: 10.7506/spkx1002-6630-20221124-288

大鲵肽联合跑台运动对肥胖大鼠的减重效果及机制

钱锦，方双喜，胡雅婷，马坤，刘辉，贾绍辉

（1.武汉体育学院运动医学院，湖北武汉 430079；2.济南大学体育学院，山东济南 250024；3.运动训练监控湖北省重点实验室，天久运动营养食品研发中心，湖北武汉 430079）

出版日期:2024-03-15 发布日期:2024-04-03
基金资助:
湖北省教育厅科学研究计划项目（B2019193）；武汉体育学院中青年科研创新团队项目（21KT14）

Effect and Mechanism of Andrias davidianus Peptide Supplementation Combined with Treadmill Exercise on Body Mass Loss in Obese Rats

QIAN Jin, FANG Shuangxi, HU Yating, MA Kun, LIU Hui, JIA Shaohui

(1. School of Sports Medicine, Wuhan Sports University, Wuhan 430079, China; 2. Institute of Physical Education, University of Jinan, Jinan 250024, China; 3. Hubei Key Laboratory of Exercise Training and Monitoring, Tianjiu Research and Development Center for Exercise Nutrition and Foods, Wuhan 430079, China)

Online:2024-03-15 Published:2024-04-03

摘要/Abstract

摘要： 目的：探讨补充大鲵肽（Andrias davidianus peptide，ADP）联合跑台运动治疗肥胖的作用及潜在机制。方法：采用高脂饲料（high fat diet，HFD）喂养构建肥胖模型大鼠，然后将肥胖大鼠随机分为模型组（HFD组）、跑台运动组（Ex组）、ADP喂养组（ADP组）和ADP喂养联合运动组（ADP＋Ex组）。Ex组大鼠实施为期6 周的跑台运动，ADP组灌胃给予6 周大鲵肽，ADP＋Ex组大鼠给予ADP同时联合跑台运动干预，以普通SD大鼠作为对照（CN）组。每周记录体质量和食物摄入量，采用酶联免疫吸附试验（enzyme-linked immunosorbent assay，ELISA）检测血清胰高血糖素样肽-1（glucagon-like peptide-1，GLP-1）、神经肽Y（neuropeptide Y，NPY）、鸢尾素（Irisin）、瘦素（Leptin）、甘油三酯（triglyceride，TG）、总胆固醇（total cholesterol，TC）、低密度脂蛋白（low density lipoprotein，LDL）和高密度脂蛋白（high density lipoprotein，HDL）的水平，Western blot检测食欲调节蛋白（NPY和阿黑皮素原（proopiomelanocortin，POMC））和能量代谢相关蛋白的表达。结果：与模型组相比，ADP联合跑台运动组大鼠体质量、腹股沟和附睾周围的脂肪堆积、摄食量以及血清TG、TC和LDL含量均表现出显著下降。此外，ELISA结果显示，ADP联合跑台运动降低肥胖大鼠血清中NPY和Leptin水平，增加血清中Irisin和GLP-1的质量浓度。同时，Western blot结果也表明，与HFD组相比，ADP联合跑台运动可显著提高食欲抑制相关蛋白POMC和能量代谢相关蛋白（Irisin、磷酸化腺苷酸活化蛋白激酶、过氧化物酶体增殖物激活受体γ辅助因子-1α、含III型纤连蛋白域蛋白5）的表达，而下调食欲增强蛋白NPY的表达。结论：联合ADP以及有氧运动可以有效缓解高脂饮食诱导的肥胖，这可能为缓解高脂饮食引起的肥胖提供一个新的有希望的策略。

关键词: 大鲵肽；运动；食欲；能量代谢

Abstract: Objective: To evaluate the antiobesity effect and underlying mechanism of Andrias davidianus peptide (ADP) supplementation combined with treadmill exercise. Methods: A mouse model of obesity was established by feeding mice a high-fat diet (HFD), and the obese mice were randomly divided into four groups: model, treadmill exercise (Ex), ADP and ADP combined with treadmill exercise (ADP + Ex). The rats in the Ex group were subjected to a six-week treadmill exercise training. The rates in the ADP group were gavaged with ADP for six weeks. The rats in the ADP + Ex group were treated with ADP supplementation combined with treadmill exercise training. Normal Sprague Dawley (SD) rats were used as the control (CN) group. Body mass and food intake were recorded each week, and the serum levels of glucagon-like peptide-1 (GLP-1), neuropeptide Y (NPY), irisin, leptin, triglycerides (TG), total cholesterol (TC), low density lipoprotein (LDL) and high density lipoprotein (HDL) were investigated by enzyme-linked immunosorbent assay (ELISA). Western blot was used to detect the expression of appetite-regulated proteins (NPY and proopiomelanocortin (POMC)) and energy metabolism-related proteins. Results: Body mass, inguinal and epididymal fat accumulation, food intake, and serum TG, TC and LDL levels significantly decreased in the ADP + Ex group compared with the model group. In addition, ELISA results revealed that combination of ADP supplementation with treadmill exercise reduced the serum levels of NPY and leptin but increased the serum concentrations of GLP-1 and irisin in obese rats. Western blot analysis showed that ADP supplementation combined with treadmill exercise greatly up-regulated the expression of POMC and energy metabolism related proteins (irisin, phospho-adenosine monophosphate-activated protein kinase, peroxisome proliferator-activated receptor γ co-activated factor-1α, and fibronectin type III domain containing protein 5), but down-regulated the expression of NPY. Conclusion: The combination of ADP with aerobic exercise can effectively alleviate HFD-induced obesity, thereby providing a new promising strategy to alleviate HFD-induced obesity.

Key words: Andrias davidianus peptide; exercise; appetite; energy metabolism

中图分类号:

TS201.4

钱锦，方双喜，胡雅婷，马坤，刘辉，贾绍辉. 大鲵肽联合跑台运动对肥胖大鼠的减重效果及机制[J]. 食品科学, 2024, 45(5): 69-77.

QIAN Jin, FANG Shuangxi, HU Yating, MA Kun, LIU Hui, JIA Shaohui. Effect and Mechanism of Andrias davidianus Peptide Supplementation Combined with Treadmill Exercise on Body Mass Loss in Obese Rats[J]. FOOD SCIENCE, 2024, 45(5): 69-77.

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大鲵肽联合跑台运动对肥胖大鼠的减重效果及机制

Effect and Mechanism of Andrias davidianus Peptide Supplementation Combined with Treadmill Exercise on Body Mass Loss in Obese Rats

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