食品科学 ›› 2023, Vol. 44 ›› Issue (24): 34-40.doi: 10.7506/spkx1002-6630-20230404-032

• 食品化学 • 上一篇    下一篇

负载鳙鱼肽的壳聚糖/三聚磷酸钠和壳聚糖/亚麻籽胶复合纳米颗粒的稳定性和生物相容性评价

郑昌亮, 陈梦婷, 汪兰, 曲映红, 施文正, 石柳, 陈胜, 乔宇, 李新, 郭晓嘉, 吴文锦, 杨玉平   

  1. (1.湖北省农业科学院农产品加工与核农技术研究所,农业农村部农产品冷链物流技术重点实验室,湖北 武汉 430064;2.上海海洋大学食品学院,上海 201306;3.武汉药品医疗器械检验所,湖北 武汉 430075)
  • 出版日期:2023-12-25 发布日期:2024-01-02
  • 基金资助:
    “十三五”国家重点研发计划重点专项(2019YFD0901805); 湖北省农机装备补短板核心技术应用攻关项目(HBSNYT202221)

Evaluation of Stability and Biocompatibility of Chitosan/Sodium Tripolyphosphate and Chitosan/Flaxseed Gum Composite Nanoparticles Loaded with Bighead Carp Peptides

ZHENG Changliang, CHEN Mengting, WANG Lan, QU Yinghong, SHI Wenzheng, SHI Liu, CHEN Sheng, QIAO Yu, LI Xin, GUO Xiaojia, WU Wenjin, YANG Yuping   

  1. (1. Institute of Agricultural Products Processing and Nuclear Agricultural Technology, Hubei Academy of Agricultural Sciences, Key Laboratory of Cold Chain Logistics Technology for Agro-product, Ministry of Agriculture and Rural Affairs, Wuhan 430064, China;2. College of Food Sciences and Technology, Shanghai Ocean University, Shanghai 201306, China;3. Wuhan Institute for Drug and Medical Device Control, Wuhan 430075, China)
  • Online:2023-12-25 Published:2024-01-02

摘要: 壳聚糖纳米颗粒具有制备简单、成本低和生物相容性高等优点,正逐渐成为递送生物活性成分的优良载体。前期研究表明负载鳙鱼肽(bighead carp peptides,BCP)的壳聚糖/三聚磷酸钠(chitosan/sodium tripolyphosphate,CS/TPP)和壳聚糖/亚麻籽胶(chitosan/flaxseed gum,CS/FG)纳米颗粒具有粒径小、包埋率高和缓释效果显著等优点。本研究主要探究离子强度、pH值、模拟消化和贮藏时间对制备的CS/TPP-BCP和CS/FG-BCP纳米颗粒的影响,并以Caco-2细胞为模型,评估壳聚糖纳米颗粒处理的胞外乳酸脱氢酶的含量、体内抗氧化能力和细胞摄取度。结果表明两种壳聚糖纳米颗粒在酸性条件下比较稳定,对带相反电荷的溶液较为敏感。经过纳米颗粒负载后的多肽的稳定性高于游离肽且两种纳米颗粒都具有较高生物相容性以及细胞摄取度。

关键词: 壳聚糖;纳米颗粒;稳定性;生物相容性

Abstract: Chitosan nanoparticle is becoming an excellent carrier for the delivery of bioactive components due to the advantages of simple preparation, low cost and high biocompatibility. Previous studies have shown that chitosan/sodium tripolyphosphate (CS/TPP) and chitosan/flaxseed gum (CS/FG) nanoparticles loaded with bighead carp peptides (BCP) have the advantages of small particle size, high encapsulation rate and significant slow-release effect. This study explored the effects of ionic strength, pH, simulated digestion and storage time on the preparation of chitosan/sodium tripolyphosphate (CS/TPP-BCP) and chitosan/flaxseed gum (CS/FG-BCP) nanoparticles, and evaluated the extracellular lactate dehydrogenase content and antioxidant capacity in vivo of Caco-2 cells treated with the chitosan nanoparticles and their cellular uptake. The results showed that the two kinds of chitosan nanoparticles were stable under acidic conditions and sensitive to a solution with opposite charges. The stability of the nanoparticles loaded with bighead peptides was higher than that of free peptides and both nanoparticles showed higher biocompatibility and cell uptake.

Key words: chitosan; nanoparticle; stability; biocompatibility

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