副干酪乳杆菌FZU103对小鼠酒精性肝损伤的防控作用

doi:10.7506/spkx1002-6630-20230729-329

食品科学 ›› 2024, Vol. 45 ›› Issue (7): 135-144.doi: 10.7506/spkx1002-6630-20230729-329

副干酪乳杆菌FZU103对小鼠酒精性肝损伤的防控作用

梁梓华，李嘉仪，谢林惠，杨梓翊，尤适泽，吴超，李文龙，艾连中，倪莉，吕旭聪，陈有挺

（1.福州大学生物科学与工程学院，食品科学技术研究所，福建福州 350108；2.福州大学先进制造学院，食品营养与健康研究中心，福建晋江 362200；3.福建医科大学第一临床医学院，福建福州 350004；4.上海理工大学健康科学与工程学院，上海食品微生物工程技术研究中心，上海 200093；5.福建医科大学附属第一医院肝胆胰外科，福建福州 350004）

出版日期:2024-04-15 发布日期:2024-04-23
基金资助:
福建省教育与科研财政专项（BPB-2022CYT）；福州大学“旗山学者”高层次人才计划项目（GXRC21049）

Preventive Effect of Lactobacillus paracasei FZU103 on Alcoholic Liver Injury in Mice

LIANG Zihua, LI Jiayi, XIE Linhui, YANG Ziyi, YOU Shize, WU Chao, LI Wenlong, AI Lianzhong, NI Li, LÜ Xucong, CHEN Youting

(1. Institute of Food Science and Technology, College of Biological Science and Technology, Fuzhou University, Fuzhou 350108, China; 2. Food Nutrition and Health Research Center, School of Advanced Manufacturing, Fuzhou University, Jinjiang 362200, China; 3. The First Clinical Medical College, Fujian Medical University, Fuzhou 350004, China; 4. Shanghai Engineering Research Center of Food Microbiology, School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China; 5. Department of Hepatobiliary and Pancreatic Surgery, First Affiliated Hospital, Fujian Medical University, Fuzhou 350004, China)

Online:2024-04-15 Published:2024-04-23

摘要/Abstract

摘要： 目的：探究副干酪乳杆菌FZU103（Lactobacillus paracasei FZU103，LP-FZU103）对酒精性肝损伤（alcoholic liver injury，ALI）的防控作用。方法：选取36 只SPF级ICR小鼠，随机分为空白组、模型组、实验组（LP-FZU103干预），实验6 周后测定体质量及脏器系数、血清及肝脏生化、肝组织病理学及炎症因子、肝功能相关基因转录和肠道菌群组成。结果：与模型组相比，LP-FZU103干预可改善ALI小鼠的脏器系数和肝组织病理损伤程度，显著降低血清中总胆固醇、甘油三酯和低密度脂蛋白胆固醇浓度以及谷丙转氨酶和谷草转氨酶活性，提高血清高密度脂蛋白胆固醇浓度；显著提高肝脏中过氧化氢酶和超氧化物歧化酶活性以及谷胱甘肽含量，降低肝脏中丙二醛含量和白细胞介素6、干扰素γ水平；显著上调脂质代谢相关基因Ldlr及下调Acc1、Hmgcr和Cd36的mRNA水平；显著增加小鼠肠道中约氏乳杆菌（Lactobacillus johnsonii）、罗伊氏乳杆菌（Lactobacillus reuteri）、副干酪乳杆菌（Lactobacillus paracasei）等有益细菌的相对丰度。结论：LP-FZU103干预可一定程度防控小鼠ALI的发生，这与其改善肠道菌群及肝脏代谢功能密切相关。

关键词: 副干酪乳杆菌；酒精性肝损伤；氧化应激；肝功能基因；肠道菌群

Abstract: Objective: To explore the preventive effect of Lactobacillus paracasei FZU103 (LP-FZU103) on alcoholic liver injury (ALI). Methods: Altogether 36 specific pathogen free- (SPF-) grade ICR mice were randomly divided into three groups: control, model and experimental (LP-FZU103 intervention). After the six-week experiment, body mass, organ coefficients, serum and liver biochemical indexes, liver histopathology and inflammatory cytokines, the transcription of liver function-related genes and intestinal flora composition were measured. Results: Compared with the model group, intervention of LP-FZU103 improved the organ coefficients and pathological liver damage in ALI mice, significantly reduced the levels of total cholesterol (TC), triglyceride (TG) and low-density lipoprotein cholesterol (LDL-C) as well as the activity of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in the serum, increased the serum concentration of high-density lipoprotein cholesterol (HDL-C), significantly increased the activity of catalase (CAT) and superoxide dismutase (SOD) and the content of glutathione (GSH) in the liver, and decreased MDA content and interleukin-6 (IL-6), interferon-γ (IFN-γ) levels in the liver. Moreover, LP-FZU103 intervention significantly up-regulated the mRNA expression level of the lipid metabolism-related gene Ldlr and down-regulated the mRNA expression level of Acc1, Hmgcr and Cd36 as well as increased the relative abundance of beneficial bacteria such as Lactobacillus johnsonii, Lactobacillus reuteri and Lactobacillus paracasei in the gut of mice. Conclusion: LP-FZU103 intervention can prevent and control the occurrence of alcoholic liver injury in mice, which is closely related to the improved intestinal flora and liver metabolic function.

Key words: Lactobacillus paracasei; alcoholic liver injury; oxidative stress; liver function-related genes; intestinal flora

中图分类号:

R151.2

梁梓华，李嘉仪，谢林惠，杨梓翊，尤适泽，吴超，李文龙，艾连中，倪莉，吕旭聪，陈有挺. 副干酪乳杆菌FZU103对小鼠酒精性肝损伤的防控作用[J]. 食品科学, 2024, 45(7): 135-144.

LIANG Zihua, LI Jiayi, XIE Linhui, YANG Ziyi, YOU Shize, WU Chao, LI Wenlong, AI Lianzhong, NI Li, LÜ Xucong, CHEN Youting. Preventive Effect of Lactobacillus paracasei FZU103 on Alcoholic Liver Injury in Mice[J]. FOOD SCIENCE, 2024, 45(7): 135-144.

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副干酪乳杆菌FZU103对小鼠酒精性肝损伤的防控作用

Preventive Effect of Lactobacillus paracasei FZU103 on Alcoholic Liver Injury in Mice

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