食品科学 ›› 2024, Vol. 45 ›› Issue (12): 176-186.doi: 10.7506/spkx1002-6630-20230919-182

• 成分分析 • 上一篇    

基于风味组学和脂质组学分析不同品种原料鸭对卤鸭挥发性风味的影响

滕飞,刘小琳,钟强,邢政,吴婷,徐晓云   

  1. (1.华中农业大学食品科技学院,湖北 武汉 430072;2.环境食品学教育部重点实验室,湖北 武汉 430072;3.果蔬加工与品质调控湖北省重点实验室,湖北 武汉 430070)
  • 发布日期:2024-07-10
  • 基金资助:
    “十四五”国家重点研发计划重点专项(2021YFD2100104); 中央高校基本科研业务费专项资金资助项目(2662023SPPY001)

Flavoromic and Lipidomic Analysis of the Effect of Different Breeds on Volatile Flavor Components of Pot-Stewed Duck

TENG Fei, LIU Xiaolin, ZHONG Qiang, XING Zheng, WU Ting, XU Xiaoyun   

  1. (1. College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430072, China; 2. Key Laboratory of Environment Correlative Dietology, Ministry of Education, Wuhan 430072, China; 3. Hubei Key Laboratory of Fruit & Vegetable Processing & Quality Control, Wuhan 430070, China)
  • Published:2024-07-10

摘要: 本研究以樱桃谷鸭、荆江鸭和番鸭制成的卤鸭为研究对象,采用顶空固相微萃取-气相色谱-质谱联用技术和液相色谱-串联质谱联用技术对3 种卤鸭的挥发性有机化合物(volatile organic compounds,VOCs)和脂质进行分析,共鉴定出53 种VOCs和3 538 种脂质。此外,确定了16 种特征VOCs,筛选出1 387 种可区分3 种卤鸭的潜在脂质标志物。通过网络分析和京都基因与基因组百科全书富集分析发现,磷脂与各脂质间联系密切,含18 个以上碳原子的不饱和脂肪酸可能是脂质降解的主要产物。相关性分析结果表明,VOCs与脂质关系密切,以磷脂酰胆碱和磷脂酰乙醇胺为主的磷脂标记物可能有助于形成特征VOCs。通过聚类分析可知,荆江鸭和番鸭制成的卤鸭风味和脂质组成比较相似,本实验结果可为卤鸭风味调控和原料鸭选育提供理论依据。

关键词: 卤鸭;品种;挥发性风味物质;风味组学;脂质组学

Abstract: In this study, headspace solid-phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GC-MS) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) was employed to analyze the composition of volatile organic compounds (VOCs) and lipids in pot-stewed duck from three breeds, Cherry Valley, Jingjiang and Muscovy. A total of 53 VOCs and 3 538 lipids were identified. In addition, 16 characteristic VOCs were identified, and 1 387 potential lipid markers were selected to differentiate the three stewed ducks. Network analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis revealed that phospholipids were closely linked to lipids and unsaturated fatty acids with more than 18 carbon atoms may be the major degradation products of lipids. The results of correlation analysis showed that VOCs were closely related to lipids, and phospholipid markers, mainly composed of phosphatidylcholine (PC) and phosphatidylethanolamine (PE), may contributed to the formation of characteristic VOCs. Cluster analysis showed that the flavor and lipid compositions of stewed Jingjiang and Muscovy ducks were relatively similar. This study may provide a theoretical basis for the flavor regulation of pot-stewed duck and the selection of duck breeds for the production of pot-stewed duck.

Key words: pot-stewed duck; breeds; volatile flavor components; flavoromics; lipidomics

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