关键词: 烧鸡；气调包装；高通量测序；腐败菌；微生物多样性

Abstract: In order to explore dynamic changes in the physicochemical characteristics and microbial diversity of modified atmosphere packaged braised chicken during refrigeration, high-throughput sequencing technology and traditional isolation and identification methods were combined to study the physical and microbial changes of modified atmosphere packaged (25% CO2/75% N2) braised chicken during storage at 4 ℃ for 10 days. The results showed that total volatile basic nitrogen (TVB-N) content and total bacterial count increased significantly during storage (P < 0.05). pH decreased significantly in the late storage period (P < 0.05). Thiobarbituric acid reactive substance (TBARS) levels tended to be stable at the end of storage (P > 0.05). These changes were related to the gas composition in modified atmosphere packaging. The microbial species richness of braised chicken was the highest at the early stage of storage, and then decreased gradually. At the initial stage of storage, the relative abundance of Enterobacteriaceae, Acinetobacter and Aeromonas were high. At the middle and late stages of storage, the relative abundance of Weissella and Enterobacter were high. The relative abundance of Weissella, Enterobacter, Lactococcus and Leuconostoc increased gradually during the whole storage period and their growth was dominant. Six species of spoilage organisms were isolated and identified, namely, Lactobacillus curvatus, Bacillus cereus, Bacillus safensis, Serratia liquefaciens, Hafnia sp. and Enterobacter sp., which could be the major causes of the rot of braised chicken under modified atmosphere packaging conditions.

Key words: braised chicken; modified atmosphere packaging; high-throughput sequencing; spoilage bacteria; microbial diversity