二乙基二硫代氨基甲酸钠通过抑制超氧化物歧化酶延缓马铃薯块茎愈伤组织的形成

doi:10.7506/spkx1002-6630-20240206-050

食品科学 ›› 2024, Vol. 45 ›› Issue (23): 1-7.doi: 10.7506/spkx1002-6630-20240206-050

• 基础研究 •

二乙基二硫代氨基甲酸钠通过抑制超氧化物歧化酶延缓马铃薯块茎愈伤组织的形成

程新艳，宋兵芳，柳宁，田文茂，尹燕，李永才，毕阳，王毅

（1.甘肃农业大学食品科学与工程学院，甘肃兰州 730070；2.兰州市农业科技研究推广中心，甘肃兰州 730030）

发布日期:2024-12-06
基金资助:
国家自然科学基金地区科学基金项目（32160592）；甘肃省高等学校创新基金项目（2021A-051）；甘肃省自然科学基金项目（21JR7RA867）

Diethyldithiocarbamate Delayed Wound-Induced Callus Formation in Potato Tubers by Inhibiting Superoxide Dismutase

CHENG Xinyan, SONG Bingfang, LIU Ning, TIAN Wenmao, YIN Yan, LI Yongcai, BI Yang, WANG Yi

(1. College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, China; 2. Lanzhou Agricultural Science and Technology Research and Extension Center, Lanzhou 730030, China)

Published:2024-12-06

摘要/Abstract

摘要： 用10 mmol/L超氧化物歧化酶（superoxide dismutase，SOD）抑制剂二乙基二硫代氨基甲酸钠（diethyldithiocarbamate，DDC）浸泡处理损伤后的马铃薯块茎，测定块茎愈伤期间伤口处StSODs基因表达、SOD活性、活性氧含量、苯丙烷代谢酶活性和总酚及木质素含量，观察伤口处聚酚软木脂（suberin polyphenolic，SPP）和木质素的沉积。结果表明，DDC处理下调了块茎愈伤期间伤口处StSODs基因表达和SOD活性，减少了H2O2含量，还降低了苯丙氨酸解氨酶和过氧化物酶活性及总酚和木质素含量。愈伤1 d时，处理块茎的StCSD1、StFSD3和StMSD相对表达量分别低于对照31.90%、69.30%和61.83%，SOD活性、H2O2含量、苯丙氨酸解氨酶和过氧化物酶活性分别低于对照36.17%、15.38%、28.20%和24.90%。DDC处理减缓了块茎伤口处SPP和木质素的沉积，愈伤7 d时，处理组块茎的SPP和木质素细胞层厚度分别低于对照16.84%和23.00%。综上，DDC处理下调了马铃薯块茎伤口处StSODs的表达，抑制了SOD活性，降低了H2O2含量，削弱了苯丙烷代谢，延缓了块茎愈伤组织中SPP和木质素的积累。因此，DDC延缓马铃薯块茎愈伤组织的形成与其抑制SOD歧化产生H2O2密切相关。

关键词: 马铃薯块茎；超氧化物歧化酶；H2O2；二乙基二硫代氨基甲酸钠；愈伤

Abstract: In this study, potato tubers (Solanum tuberosum L.) were artificially damaged and then soaked in 10 mmol/L diethyldithiocarbamate (DDC) solution, an inhibitor of superoxide dismutase (SOD). We determined the StSODs gene expression and activity of SOD, reactive oxygen species (ROS) content, phenylpropanoid metabolism-related enzyme activity, total phenols and lignin contents at the wound site during the healing process. In addition, the deposition of suberin polyphenolic (SPP) and lignin were observed. The results showed that DDC treatment downregulated StSODs expression, suppressed SOD activity, reduced the content of H2O2, and decreased the activities of phenylalanine ammonia lyase (PAL) and peroxidase (POD) as well as the contents of total phenols and lignin compared with the control. After 1 day of healing, the expression of StCSD1, StFSD3 and StMSD in the treated tubers were 31.90%, 69.30% and 61.83% lower than those of the control, respectively. In addition, SOD activity, H2O2 content, PAL and POD activity were 36.17%, 15.38%, 28.20% and 24.90% lower than those of the control, respectively. The deposition of SPP and lignin were delayed by DDC treatment. After 7 days of healing, the thicknesses of the SPP and lignin layers in the treated tubers were 16.84% and 23.00% lower than those of the control, respectively. In summary, DDC treatment downregulated StSODs expression during the healing process, inhibited the activity of SOD, reduced H2O2 content, weakened phenylpropanoid metabolism, and delayed the accumulation of SPP and lignin at the wound site of potato tubers. Therefore, the effect of DDC on delaying callus formation in wounded potato tubers is closely related to its inhibitory effect on H2O2 production catalyzed by SOD.

Key words: potato tubers; superoxide dismutase; H2O2; diethyldithiocarbamate; wound healing

中图分类号:

TS215

程新艳，宋兵芳，柳宁，田文茂，尹燕，李永才，毕阳，王毅. 二乙基二硫代氨基甲酸钠通过抑制超氧化物歧化酶延缓马铃薯块茎愈伤组织的形成[J]. 食品科学, 2024, 45(23): 1-7.

CHENG Xinyan, SONG Bingfang, LIU Ning, TIAN Wenmao, YIN Yan, LI Yongcai, BI Yang, WANG Yi. Diethyldithiocarbamate Delayed Wound-Induced Callus Formation in Potato Tubers by Inhibiting Superoxide Dismutase[J]. FOOD SCIENCE, 2024, 45(23): 1-7.

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二乙基二硫代氨基甲酸钠通过抑制超氧化物歧化酶延缓马铃薯块茎愈伤组织的形成

Diethyldithiocarbamate Delayed Wound-Induced Callus Formation in Potato Tubers by Inhibiting Superoxide Dismutase

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