关键词: 水溶性壳聚糖；可得然胶；灰葡萄孢霉菌；抑菌活性；抑菌机理

Abstract: In this study, the inhibitory effect of an edible composite composed of coating water-soluble chitosan and curdlan on postharvest infection of cherry tomatoes by Botrytis cinerea and the underlying mechanism was investigated. The mycelial growth, minimum inhibitory concentration (MIC), spore germination and membrane permeability of B. cinerea were studied. The results showed that the composite coating had a significant inhibitory effect on the growth of B. cinerea. After treatment with the composite coating, the cell membrane was damaged, resulting in leakage of intracellular contents and hyphal shrinkage. Meanwhile, it significantly inhibited the expansion of B. cinerea lesions. The composite coating might inhibit B. cinerea mainly by affecting the cell wall and membrane. These results suggest that the water-soluble chitosan/curdlan edible composite coating can be used as an effective antimicrobial material in the storage and preservation of cherry tomatoes.

Key words: water-soluble chitosan; curdlan; Botrytis cinerea; antifungal activity; antifungal mechanism