食品科学 ›› 2024, Vol. 45 ›› Issue (23): 232-241.doi: 10.7506/spkx1002-6630-20240602-003

• 包装贮运 • 上一篇    

电子鼻结合气相色谱-离子迁移谱联用技术分析松茸货架期间不同部位挥发性风味物质的变化

孟凡,王艺儒,赵悦辰,关文强,王璇,何兴兴,姚刚,胡昕   

  1. (1.天津商业大学生物技术与食品科学学院,天津市食品生物技术重点实验室,天津 300134;2.深圳顺丰泰森控股(集团)有限公司,广东 深圳 518000)
  • 发布日期:2024-12-06
  • 基金资助:
    国家级大学生创新创业训练计划项目(202310069002); 深圳顺丰泰森控股(集团)有限公司委托项目(90202109100239)

Changes of Volatile Flavor Compounds in Different Parts of Tricholoma matsutake during Shelf Life Analyzed by Electronic Nose Combined with Gas Chromatography-Ion Mobility Spectrometry

MENG Fan, WANG Yiru, ZHAO Yuechen, GUAN Wenqiang, WANG Xuan, HE Xingxing, YAO Gang, HU Xin   

  1. (1. Tianjin Key Laboratory of Food Biotechnology, School of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, China; 2. Shenzhen SF Tyson Holding (Group) Co. Ltd., Shenzhen 518000, China)
  • Published:2024-12-06

摘要: 为探究松茸在货架期间不同部位(菌盖、菌柄)的气味轮廓及变化趋势,采用电子鼻与气相色谱离子迁移谱(gas chromatography-ion mobility spectrometry,GC-IMS)技术,结合主成分分析和聚类热图,对不同货架期松茸的挥发性风味物质的变化进行分析。结果表明,在松茸货架期间,菌柄和菌盖共检出48 种挥发性物质,包括10 种醛、6 种醇、5 种酮、4 种含硫化合物、9 种酯、2 种酸、3 种烯烃、其他类挥发性物质如杂环化合物等9 种,以及15 种未被识别,且菌柄中检出的挥发性物质种类比菌盖丰富。总体而言,松茸货架期间挥发性物质的种类和含量持续减少,菌盖中挥发性物质变化的拐点出现在货架24 h左右,而菌柄则分别在货架12 h和48 h左右出现拐点。电子鼻和GC-IMS联用可快速直观地对不同货架时间的松茸(盖、柄)挥发性物质进行差异分析,进而有助于判断松茸的新鲜度。

关键词: 松茸;货架期;电子鼻;气相色谱-离子迁移谱联用;挥发性风味物质

Abstract: In order to investigate trends in the odor profiles of different parts (cap and stipe) of Tricholoma matsutake during the shelf-life, the present study used an electronic nose and gas chromatography-ion mobility spectrometry (GC-IMS) combined with principal component analysis (PCA) and cluster analysis to analyze the changes of volatile flavor substances in T. matsutake at different points of the shelf-life. The results showed that a total of 48 volatile organic compounds (VOCs), including 10 aldehydes, 6 alcohols, 5 ketones, 4 sulfur-containing compounds, 9 esters, 2 acids, 3 alkenes, 9 other volatile substances such as heterocyclic compounds, and 15 unidentified volatile compounds, were detected. The stipe contained a richer variety of volatile substances than the cap. In general, the types and contents of volatile substances in T. matsutake continued to decrease during the shelf-life period. The inflection point of volatile substances occurred after around 24 h for the cap, and after both around 12 and 48 h for the stipe. In conclusion, the combination of electronic nose and GC-IMS can be used to quickly and intuitively analyze the differences in volatiles between the cap and stipe of T. matsutake at different time points of the shelf-life, thereby helping to determine the freshness of T. matsutake.

Key words: Tricholoma matsutake; shelf life; electronic nose; gas chromatography-ion mobility spectrometry; volatile flavor compounds

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