嗜果聚糖Lactiplantibacillus plantarum 19M03的筛选及全基因组测序分析

doi:10.7506/spkx1002-6630-20240512-089

食品科学 ›› 2024, Vol. 45 ›› Issue (23): 113-122.doi: 10.7506/spkx1002-6630-20240512-089

• 生物工程 • 上一篇

嗜果聚糖Lactiplantibacillus plantarum 19M03的筛选及全基因组测序分析

孙大庆，齐贺，邸子清，葛相麟，洪青平，杜新蕊，姚禹西，李洪飞

（1.黑龙江八一农垦大学国家杂粮工程技术研究中心，黑龙江大庆 163319；2.黑龙江八一农垦大学食品学院，黑龙江大庆 163319；3.黑龙江省农产品加工与质量安全重点实验室，黑龙江大庆 163319）

发布日期:2024-12-06
基金资助:
黑龙江八一农垦大学引进人才科研启动计划项目（XDB202009）；黑龙江八一农垦大学三横三纵支持计划项目（ZRCPY202005）；黑龙江八一农垦大学研究生创新科研项目（YJSCX2022-Y53）；黑龙江八一农垦大学引进人才科研启动基金项目（XDB202404）；黑龙江八一农垦大学“三纵”科研支持计划基础培育项目（ZRCPY202228）

Screening and Whole-Genome Sequencing Analysis of Fructofuranose-Metabolizing Lactiplantibacillus plantarum 19M03

SUN Daqing, QI He, DI Ziqing, GE Xianglin, HONG Qingping, DU Xinrui, YAO Yuxi, LI Hongfei

(1. National Coarse Cereals Engineering Research Center, Heilongjiang Bayi Agricultural University, Daqing 163319, China; 2. College of Food Science, Heilongjiang Bayi Agricultural University, Daqing 163319, China; 3. Key Laboratory of Agro-products Processing and Quality Safety of Heilongjiang Province, Daqing 163319, China)

Published:2024-12-06

摘要/Abstract

摘要： 为探究乳酸菌果聚糖代谢机制和开发低果聚糖发酵食品，本研究对287 株酸面团来源的乳酸菌进行果聚糖利用能力筛选，获得1 株嗜果聚糖乳酸菌19M03，之后利用高通量测序和生物信息学技术对该菌株进行全基因组测序和果聚糖代谢机制分析。测序结果表明，菌株19M03基因组含有1 个染色体和9 个质粒，基因组全长3 246 316 bp，GC相对含量44.53%，包含3 084 个编码基因、66 个tRNA和16 个rRNA。系统发育关系分析表明，分类学上菌株19M03属于植物乳植杆菌。基因功能注释结果表明，在同源蛋白簇、基因本体论、京都基因与基因组百科全书（Kyoto Encyclopedia of Genes and Genomes，KEGG）和碳水化合物活性酶数据库中，19M03共编码357 个碳水化合物代谢相关基因和351 个碳水化合物转运相关基因，其中与果聚糖代谢和转运相关的基因均有12 个。根据这些基因在KEGG数据库中的代谢关系，提出19M03果聚糖代谢模型。该模型解析了果聚糖水解过程及其催化反应的细胞定位（既可胞内，又可胞外）、细胞膜中运输低聚果糖和果糖的特异转运蛋白（IIABCFOS、IIABCDfru、IIABCfru）、细胞内果糖分解代谢途径（n＝4）和果糖合成代谢途径（n＝1）及其所有相关酶的编码基因。此外，耐药和毒力基因分析证明19M03具有良好的安全性。总之，本研究筛选获得了1 株嗜果聚糖乳酸菌19M03，并在全基因组水平揭示了19M03果聚糖代谢机制，这些结果可为阐明乳酸菌果聚糖代谢机制提供重要的理论参考，为今后低果聚糖功能食品的开发和应用提供优质的菌种资源。

关键词: 果聚糖代谢；全基因组测序；代谢机制

Abstract: This study was conducted to investigate the mechanism of fructan metabolism in lactic acid bacteria in order to develop low-fructan fermented foods. One strain capable of utilizing fructan, 19M03, was selected from 287 lactic acid bacteria strains isolated from sourdough. Then, the strain was subjected to whole-genome sequencing and its fructan metabolism mechanism was analyzed by using high-throughput sequencing and bioinformatic techniques. The sequencing results showed that the genome of strain 19M03 contained one chromosome and nine plasmids, with a total length of 3 246 316 bp, GC content of 44.53%, 3 084 coding genes, 66 tRNAs and 16 rRNAs. The phylogenetic relationship analysis showed that strain 19M03 belonged to Lactiplantibacillus plantarum. The results of gene function annotation showed that its genome sequence contained a total of 357 genes encoding carbohydrate-active enzymes (CAZymes) and 351 genes encoding carbohydrate transport proteins in the Clusters of Orthologous Groups of proteins (COGs), Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) and CAZy databases, as well as 12 genes related to fructan metabolism and transport, respectively. Based on the metabolic relationship of these genes in the KEGG database, a fructan metabolism model was proposed for 19M03. The model resolved the cellular localization of the fructan hydrolysis process and related catalytic reactions (both intracellular and extracellular), the specific transporters of fructooligosaccharide and fructose in the cell membrane (IIABCFOS, IIABCDfru and IIABCfru), the intracellular pathways of fructose catabolism (n = 4) and fructose anabolism (n = 1), and the genes encoding all related enzymes. In addition, resistance and virulence gene analyses demonstrated that 19M03 had a good safety profile. In conclusion, these results provide an important theoretical basis for elucidating the fructose metabolism mechanism of lactic acid bacteria so as to provide high-quality strain resources for the development and application of low-fructofuran functional foods in the future.

Key words: fructan metabolism; whole-genome sequencing; metabolic mechanism

中图分类号:

TS201.3

孙大庆，齐贺，邸子清，葛相麟，洪青平，杜新蕊，姚禹西，李洪飞. 嗜果聚糖Lactiplantibacillus plantarum 19M03的筛选及全基因组测序分析[J]. 食品科学, 2024, 45(23): 113-122.

SUN Daqing, QI He, DI Ziqing, GE Xianglin, HONG Qingping, DU Xinrui, YAO Yuxi, LI Hongfei. Screening and Whole-Genome Sequencing Analysis of Fructofuranose-Metabolizing Lactiplantibacillus plantarum 19M03[J]. FOOD SCIENCE, 2024, 45(23): 113-122.

[1]	刘青青张明杨雯静刘可李凡李学鹏励建荣张德福. 一株多药耐药副溶血弧菌噬菌体vB_VpP_3的生物学特性及全基因组分析[J]. 食品科学, 2024, 45(24): 0-0.
[2]	刘子豪倪皓洁李文璐王凤忠王彦波曾黉. 乳酸菌基因组规模代谢建模研究与在食品体系中的应用进展[J]. 食品科学, 2024, 45(24): 0-0.
[3]	孙大庆齐贺邸子清葛相麟洪青平杜新蕊姚禹西李洪飞. 嗜果聚糖Lactiplantibacillus plantarum 19M03的筛选及全基因组测序分析[J]. 食品科学, 0, (): 0-0.
[4]	周奎宫梦学王润东李学鹏邓义佳励建荣. 冷鲜波动温度对生食三文鱼肌肉代谢物的影响[J]. 食品科学, 0, (): 0-0.
[5]	孟维民高雅鑫胡淼张凤霞文伟张鹏飞李淑英王凤忠. 豆渣的高值化利用：生物转化途径[J]. 食品科学, 0, (): 0-0.
[6]	吴梵马国文曾照中洪维鍊倪丹郭宇星. 副干酪乳酪杆菌K56表面蛋白及其黏附性能的研究[J]. 食品科学, 0, (): 0-0.
[7]	戚少含陈穗李向丽赵力超谭贵良. 发酵食品中噬菌体多样性、辅助代谢功能及宿主互作研究进展[J]. 食品科学, 0, (): 0-0.
[8]	刘芳舒志强王共明井月欣赵云苹徐英江矫春娜张健. 产褐藻胶裂解酶菌株S10的鉴定、全基因组测序及分析[J]. 食品科学, 0, (): 0-0.
[9]	梁悦琪张玉姣代艺伟陈映羲董亮张素芳. 基于全基因组测序解析贝莱斯芽孢杆菌产胞外多糖的分子基础[J]. 食品科学, 0, (): 0-0.
[10]	陈志娜尹琳琳刘锦邵孟元叶韬黄晓辰. 一株抑MRSA植物乳杆菌CHEN1的全基因测序及产细菌素基因分析[J]. 食品科学, 0, (): 0-0.
[11]	修梁光月程磊. 基于UPLC-Q/TOF-MS技术分析多种菌种发酵桑叶化学成分的变化[J]. 食品科学, 0, (): 0-0.
[12]	王宇航周玲玲周瑶玲撒俊梦张元臣马增军连凯琪. 抗菌肽LL-1对沙门氏菌的抗菌机制[J]. 食品科学, 0, (): 0-0.
[13]	陈玉瑶王元楷曾全恒周纯洁蔡甜陈科伟. 高脂饮食诱导小鼠粪菌体外发酵叶绿素a/b后的肠道菌群及代谢组分析[J]. 食品科学, 0, (): 0-0.
[14]	肖灵唐琳琳宋晓妍张艺华韩雪梅吕厚姣梁武王艳萍耿伟涛贾龙刚. 后生元调节肠道微生态及减少脑内Aβ沉积改善AD大鼠的认知障碍[J]. 食品科学, 0, (): 0-0.
[15]	王川赵雨琳李娜附俊杰刘军廖钰婷李丽曹荣. 基于风味导向解析四川晒醋特征风味物质及代谢途径[J]. 食品科学, 0, (): 0-0.

嗜果聚糖Lactiplantibacillus plantarum 19M03的筛选及全基因组测序分析

Screening and Whole-Genome Sequencing Analysis of Fructofuranose-Metabolizing Lactiplantibacillus plantarum 19M03

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价