基于干细胞因子/C-kit信号通路探讨鼠李糖乳酪杆菌Glory LG12的润肠通便作用

doi:10.7506/spkx1002-6630-20240710-109

食品科学 ›› 2025, Vol. 46 ›› Issue (3): 20-26.doi: 10.7506/spkx1002-6630-20240710-109

• 益生菌资源挖掘、功能评价及应用专栏 • 上一篇下一篇

基于干细胞因子/C-kit信号通路探讨鼠李糖乳酪杆菌Glory LG12的润肠通便作用

项芳琴，李玉星，李柏良

（东北农业大学食品学院，黑龙江哈尔滨 150006）

出版日期:2025-02-15 发布日期:2024-12-30
基金资助:
中国博士后科学基金特别资助项目（2024T140106）；国家自然科学基金青年科学基金项目（32101919）

Laxative Effect of Lacticaseibacillus rhamnosus Glory LG12: from the Perspective of the Stem Cell Factor/C-kit Signaling Pathway

XIANG Fangqin, LI Yuxing, LI Bailiang

(College of Food Science, Northeast Agricultural University, Harbin 150006, China)

Online:2025-02-15 Published:2024-12-30

摘要/Abstract

摘要： 目的：基于干细胞因子（stem cell factor，SCF）/C-kit信号通路探讨鼠李糖乳酪杆菌Glory LG12的润肠通便作用。方法：雄性ICR小鼠随机分为空白组、模型组以及鼠李糖乳酪杆菌Glory LG12低剂量（1.5×106 CFU/只）、中剂量（1.5×107 CFU/只）和高剂量（1.5×108 CFU/只）组，各组小鼠分别连续灌胃相应的药物15 d后，灌胃盐酸洛哌丁胺混悬液（4 mg/kg）建立便秘模型，测定各组小鼠的体质量、首粒黑便排出时间、5 h内黑便排出数量及质量、小肠推进率、血清中胃肠调节肽和炎性细胞因子水平及小鼠结肠组织SCF、C-kit转录水平，并观察结肠病理结构。结果：与模型组相比，中、高剂量的鼠李糖乳酪杆菌Glory LG12均能缩短小鼠的首粒黑便排出时间（P＜0.001），增加小鼠5 h内排黑便的粒数和质量（P＜0.01、P＜0.001），提高小肠内的墨汁推进率（P＜0.001）；升高小鼠血清中胃动素、胃泌素、P物质和白细胞介素（interleukin，IL）10的质量浓度，降低内皮素1、生长抑素、血管活性肠肽和IL-6的质量浓度；增加结肠组织中SCF和C-kit mRNA相对表达量（P＜0.01、P＜0.001）；修复受损结肠屏障。结论：鼠李糖乳酪杆菌Glory LG12能够通过调节SCF/C-kit通路，进而影响胃肠调节肽和炎性细胞因子的释放，发挥其促进胃肠蠕动的功能，改善便秘症状。

关键词: 鼠李糖乳酪杆菌Glory?LG12；便秘；润肠通便效果；炎性细胞因子；胃肠调节肽；干细胞因子/C-kit信号通路

Abstract: Purpose: To explore the laxative effect of Lacticaseibacillus rhamnosus Glory LG12 from the perspective of the stem cell factor (SCF)/C-kit signaling pathway. Methods: Male ICR mice were randomly divided into five groups: control, model, low- (1.5 × 106 CFU/mouse), medium- (1.5 × 107 CFU/mouse), and high- (1.5 × 108 CFU/mouse) dose L. rhamnosus Glory LG12. After 15 continuous days of gavage, all mice were further gavaged with loperamide hydrochloride suspension (4 mg/kg) to establish a mouse model of constipation. Body mass, the time taken until the first black stool excretion, the number and mass of black stool within 5 hours, small intestinal transit rate, serum levels of gastrointestinal regulatory peptides and inflammatory cytokines, colonic SCF and C-kit transcription levels were measured, and colonic pathology was examined. Results: Compared with the model group, medium- and high-dose L. rhamnosus Glory LG12 significantly reduced the time taken until the first black stool excretion (P < 0.001), increased the number and mass of black stools within 5 h (P < 0.01 and P < 0.001, respectively), and improved the ink propulsion rate in the small intestine (P < 0.001). In addition, both treatments elevated the serum levels of motilin (MTL), gastrin (Gas), substance P (SP), and interleukin 10 (IL-10), reduced the levels of endothelin-1 (ET-1), somatostatin (SS), vasoactive intestinal peptide (VIP), and IL-6, up-regulated the relative mRNA expression of SCF and C-kit in colonic tissue was (P < 0.01, P < 0.001), and restored damaged colonic barrier. Conclusion: L. rhamnosus Glory LG12 promotes gastrointestinal motility by affecting the release of gastrointestinal regulatory peptides and inflammatory cytokines through the modulation of the SCF/C-kit signaling pathway, thereby alleviating constipation symptoms.

Key words: Lacticaseibacillus rhamnosus Glory LG12; constipation; laxative effect; inflammatory cytokines; gastrointestinal regulatory peptides; stem cell factor/C-kit signaling pathway

中图分类号:

TS201.4

项芳琴，李玉星，李柏良. 基于干细胞因子/C-kit信号通路探讨鼠李糖乳酪杆菌Glory LG12的润肠通便作用[J]. 食品科学, 2025, 46(3): 20-26 https://doi.org/10.7506/spkx1002-6630-20240710-109

XIANG Fangqin, LI Yuxing, LI Bailiang. Laxative Effect of Lacticaseibacillus rhamnosus Glory LG12: from the Perspective of the Stem Cell Factor/C-kit Signaling Pathway[J]. FOOD SCIENCE, 2025, 46(3): 20-26 https://doi.org/10.7506/spkx1002-6630-20240710-109

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基于干细胞因子/C-kit信号通路探讨鼠李糖乳酪杆菌Glory LG12的润肠通便作用

Laxative Effect of Lacticaseibacillus rhamnosus Glory LG12: from the Perspective of the Stem Cell Factor/C-kit Signaling Pathway

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