亚精胺及其抑制剂对冷藏甜樱桃果实贮藏品质及其活性氧代谢的影响

doi:10.7506/spkx1002-6630-20240815-117

食品科学 ›› 2025, Vol. 46 ›› Issue (5): 292-300.doi: 10.7506/spkx1002-6630-20240815-117

亚精胺及其抑制剂对冷藏甜樱桃果实贮藏品质及其活性氧代谢的影响

齐英舰，张国强，王锋，杨青珍

（1.山西农业大学食品科学与工程学院，山西太谷 030801；2.运城学院生命科学系，运城学院特色果品品质调控与应用实验室，山西运城 044000；3.运城市果业发展中心，山西运城 044000）

出版日期:2025-03-15 发布日期:2025-02-07
基金资助:
山西省科技厅自由探索面上项目（202303021211112）；山西省高等学校科技创新计划创新平台项目（2022P012）；山西省现代农业产业技术体系建设项目（2024CYJSTX07-20）；山西省研究生教育教学改革课题项目（2022YJJG271）；食品科学与工程重点学科项目（XK-2021012；XK-2021015）

Effects of Spermidine and Its Inhibitors on the Storage Quality and Reactive Oxygen Species Metabolism of Sweet Cherry Fruits during Refrigerated Storage

QI Yingjian, ZHANG Guoqiang, WANG Feng, YANG Qingzhen

(1. College of Food Science and Engineering, Shanxi Agricultural University, Taigu 030801, China; 2. Characteristic Fruit Quality Control and Application Laboratory, Life Science Department, Yuncheng University, Yuncheng 044000, China; 3. Fruit Development Center in Yuncheng City, Yuncheng 044000, China)

Online:2025-03-15 Published:2025-02-07

摘要/Abstract

摘要： 为探究亚精胺（spermidine，SPD）对甜樱桃采后果实凹陷、贮藏品质及活性氧代谢的影响，用SPD及两种多胺合成抑制剂甲基乙二醛双脒基腙（methylglyoxal-bis(guanylhydrazone)，MGBG）和二环己胺（dicyclohexylamine，DCHA）分别对‘萨米脱’甜樱桃进行处理，定期测定相关理化指标。结果表明，SPD处理能够显著抑制甜樱桃贮藏期间凹陷率、凹陷指数及腐烂率的上升，保持较高果实硬度、可溶性固形物和可滴定酸含量。贮藏结束时，SPD处理组果实凹陷率、凹陷指数和腐烂率分别比对照组低28.69%、27.28%、19.97%，果实硬度、可溶性固形物和可滴定酸含量分别比对照组高38.74%、9.23%、11.20%。同时，SPD处理促进了甜樱桃内源SPD、腐胺（putrescine，PUT）和精胺（spermine，SPM）的积累，显著提高了甜樱桃贮藏期间超氧化物歧化酶、谷胱甘肽还原酶、过氧化氢酶和过氧化物酶活性，促进了总酚及还原型谷胱甘肽积累，并保持较高水平的自由基清除能力，有效抑制了超氧阴离子、过氧化氢和丙二醛积累，减缓了相对电导率上升。而MGBG和DCHA处理均抑制了甜樱桃内源SPD、PUT和SPM积累，降低了抗氧化酶活性和抗氧化物质含量，抑制了自由基清除能力，造成活性氧大量积累，引发细胞膜的损伤，导致果实凹陷率及腐烂率显著增加，果实品质严重下降。综上，SPD处理能显著提高甜樱桃冷藏期间抗氧化能力，减轻活性氧对细胞膜的损伤，从而抑制果实凹陷的发生，保持果实较高的贮藏品质。

关键词: 甜樱桃；凹陷；亚精胺；活性氧代谢；贮藏品质

Abstract: To investigate the effect of spermidine (SPD) on postharvest fruit pitting, storage quality and reactive oxygen (ROS) metabolism in sweet cherry, ‘Summit’ sweet cherry fruits were treated with one of SPD and two polyamine synthesis inhibitors: methylglyoxal-bis(guanylylhydrazone) (MGBG) and dicyclohexylamine (DCHA) before storage. Physicochemical indices were determined periodically during storage. The results showed that SPD treatment significantly inhibited the increase in pitting rate, pitting index and decay rate during storage, while maintaining high fruit firmness, soluble solid content and titratable acidity. At the end of storage, the pitting rate, pitting index and decay rate of SPD-treated fruits were 28.69%, 27.28% and 19.97% lower than those of the control group, respectively. The fruit firmness, soluble solid content and titratable acidity were 38.74%, 9.23% and 11.20% higher than those of the control group, respectively. Furthermore, SPD treatment promoted the accumulation of the endogenous SPD, putrescine (PUT) and spermine (SPM), significantly enhanced the activities of superoxide dismutase (SOD), glutathione reductase (GR), catalase (CAT) and peroxidase (POD), promoted the accumulation of total phenolics and reduced glutathione, and maintained high free radical scavenging capacity, thereby effectively inhibiting the accumulation of superoxide anion, hydrogen peroxide and malondialdehyde (MDA). In addition, it slowed down the increase in relative conductivity. On the contrary, MGBG and DCHA treatments inhibited the accumulation of endogenous SPD, PUT, and SPM in sweet cherry fruits, reduced the activity of antioxidant enzymes and the contents of antioxidants, and inhibited free radical scavenging capacity, thereby resulting in the accumulation of large amounts of ROS, causing cell membrane damage, and leading to a significant increase in the rate of fruit pitting and decay and consequently a significant decline in fruit quality. In conclusion, SPD treatment could significantly improve the antioxidant capacity of sweet cherry fruits during cold storage and reduce the damage caused by ROS to the cellular membrane, thereby inhibiting the occurrence of fruit pitting and consequently maintaining high fruit quality during storage.

Key words: sweet cherries; pitting; spermidine; reactive oxygen species metabolism; storage quality

中图分类号:

TS255.3

齐英舰，张国强，王锋，杨青珍. 亚精胺及其抑制剂对冷藏甜樱桃果实贮藏品质及其活性氧代谢的影响[J]. 食品科学, 2025, 46(5): 292-300 https://doi.org/10.7506/spkx1002-6630-20240815-117

QI Yingjian, ZHANG Guoqiang, WANG Feng, YANG Qingzhen. Effects of Spermidine and Its Inhibitors on the Storage Quality and Reactive Oxygen Species Metabolism of Sweet Cherry Fruits during Refrigerated Storage[J]. FOOD SCIENCE, 2025, 46(5): 292-300 https://doi.org/10.7506/spkx1002-6630-20240815-117

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亚精胺及其抑制剂对冷藏甜樱桃果实贮藏品质及其活性氧代谢的影响

Effects of Spermidine and Its Inhibitors on the Storage Quality and Reactive Oxygen Species Metabolism of Sweet Cherry Fruits during Refrigerated Storage

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