二十二碳六烯酸对阿尔茨海默病果蝇寿命的影响

doi:10.7506/spkx1002-6630-20240905-044

食品科学 ›› 2025, Vol. 46 ›› Issue (6): 156-163.doi: 10.7506/spkx1002-6630-20240905-044

二十二碳六烯酸对阿尔茨海默病果蝇寿命的影响

别娜娜，王红彩，刘思于，刘洋，孙梦菲，李普森，高璐璐，刘晓萌

（新乡医学院公共卫生学院，河南新乡 453003）

出版日期:2025-03-25 发布日期:2025-03-10
基金资助:
河南省自然科学基金青年科学基金项目（242300421536）；2024年河南省大学生创新创业训练计划项目（202410472024）

Effect of Docosahexaenoic Acid on the Lifespan of Drosophila melanogaster with Alzheimer’s Disease

BIE Nana, WANG Hongcai, LIU Siyu, LIU Yang, SUN Mengfei, LI Pusen, GAO Lulu, LIU Xiaomeng

(School of Public Health, Xinxiang Medical University, Xinxiang 453003, China)

Online:2025-03-25 Published:2025-03-10

摘要/Abstract

摘要： 目的：探究二十二碳六烯酸（docosahexaenoic acid，DHA）能否通过提高抗氧化能力而延长阿尔茨海默病（Alzheimer’s disease，AD）果蝇的寿命。方法：野生型雄性果蝇为正常组，AD雄性果蝇随机分为模型组，0.025、0.05 mmol/L和0.1 mmol/L DHA干预组。利用爬行实验检测各组果蝇运动能力；应用实时聚合酶链式反应（polymerase chain reaction，PCR）检测果蝇中SOD、CAT的表达量，并测定果蝇的抗氧化能力；利用实时PCR检测果蝇中Sirt2、Rpn11、MTH基因的表达量，并统计各组果蝇平均生存时间和生存曲线。结果：果蝇的爬行能力结果显示，与正常组相比，AD果蝇爬行指数降低（P＜0.01）；与模型组相比，0.05 mmol/L DHA干预显著提高果蝇的爬行能力（P＜0.05）；实时PCR结果显示，与模型组相比，0.05 mmol/L DHA组果蝇的SOD和CAT基因表达上调（P＜0.05），且总抗氧化能力和超氧化物歧化酶活性显著高于模型组（P＜0.05）；此外，DHA干预上调寿命相关基因Sirt2、Rpn11和MTH的表达（P＜0.05）；生存实验结果显示，AD果蝇生存概率显著降低，与模型组相比，0.05 mmol/L DHA组果蝇的平均生存时间显著上调（P＜0.05），生存概率高于AD果蝇。结论：DHA可提高AD果蝇的爬行能力，并提高果蝇抗氧化基因SOD和CAT的表达，增加果蝇的抗氧化能力，同时上调寿命相关基因Sirt2、Rpn11和MTH的表达，进而延长果蝇的寿命。

关键词: 二十二碳六烯酸；阿尔茨海默病；寿命；抗氧化；果蝇

Abstract: Purpose: To investigate whether docosahexaenoic acid (DHA) can prolong the life span of Drosophila melanogaster with Alzheimer’s disease (AD) by increasing the antioxidant capacity. Methods: Wild-type male D. melanogaster were assigned to a normal control group, and AD male D. melanogaster were randomly divided into four groups: model, 0.025, 0.05 and 0.1 mmol/L DHA intervention. The locomotor activity of D. melanogaster in each group was detected by tube climbing test. The expression levels of SOD and CAT in D. melanogaster were detected by real-time polymerase chain reaction (PCR), and the antioxidant capacity was measured. The expression levels of the Sirt2, Rpn11 and MTH genes were detected by real-time PCR, and the average survival time and survival curve of D. melanogaster in each group were calculated. Results: Compared with the control group, the crawling index of AD D. melanogaster decreased (P < 0.01). Compared with the model group, 0.05 mmol/L DHA treatment significantly increased the tube-climbing capacity of D. melanogaster (P < 0.05). The results of real-time PCR showed that compared with the model group, the expression levels of the SOD and CAT genes were up-regulated in the 0.05 mmol/L DHA treatment group (P < 0.05), and the total antioxidant capacity and superoxide dismutase activity significantly increased compared with the model group (P < 0.05). In addition, DHA treatment significantly up-regulated the expression levels of the lifespan-associated genes Sirt2, Rpn11 and MTH (P < 0.05). The results of survival test showed that the survival probability of AD D. melanogaster significantly dropped. Compared with the model group, the average survival time of D. melanogaster treated with 0.05 mmol/L DHA was significantly up-regulated (P < 0.05), and the survival probability was higher than that of AD D. melanogaster. Conclusion: DHA improved the crawling capacity of AD D. melanogaster and the expression of the antioxidant genes SOD and CAT, and increased the antioxidant capacity. Additionally, DHA upregulated the expression levels of the Sirt2, Rpn11 and MTH genes, thereby prolonging the lifespan of D. melanogaster.

Key words: docosahexaenoic acid; Alzheimer’s disease; lifespan; antioxidant; Drosophila melanogaster

中图分类号:

R151.1

别娜娜，王红彩，刘思于，刘洋，孙梦菲，李普森，高璐璐，刘晓萌. 二十二碳六烯酸对阿尔茨海默病果蝇寿命的影响[J]. 食品科学, 2025, 46(6): 156-163.

BIE Nana, WANG Hongcai, LIU Siyu, LIU Yang, SUN Mengfei, LI Pusen, GAO Lulu, LIU Xiaomeng. Effect of Docosahexaenoic Acid on the Lifespan of Drosophila melanogaster with Alzheimer’s Disease[J]. FOOD SCIENCE, 2025, 46(6): 156-163.

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二十二碳六烯酸对阿尔茨海默病果蝇寿命的影响

Effect of Docosahexaenoic Acid on the Lifespan of Drosophila melanogaster with Alzheimer’s Disease

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