我国塔吉克族儿童肠道拮抗糖尿病长双歧杆菌长亚种优势菌株筛选及其体外益生特性

doi:10.7506/spkx1002-6630-20240922-169

食品科学 ›› 2025, Vol. 46 ›› Issue (10): 128-138.doi: 10.7506/spkx1002-6630-20240922-169

我国塔吉克族儿童肠道拮抗糖尿病长双歧杆菌长亚种优势菌株筛选及其体外益生特性

李思，安美玲，李珑珑，张瑞瑞，孙海龙，倪永清

（1.石河子大学食品学院，新疆特色益生菌与乳品技术八师重点实验室，新疆石河子 832003；2.新疆天润生物科技股份有限公司，新疆生产建设兵团乳制品产业创新研究院，新疆乌鲁木齐 830000；3.新疆石河子花园乳业有限公司，新疆石河子 832003）

出版日期:2025-05-25 发布日期:2025-05-07
基金资助:
新疆生产建设兵团科技计划项目（2022DB008；2023AB050）；第七师-石河子大学科技创新专项（QS2023014）；八师重点领域科技攻关项目（2024GY02）；NSFC-新疆联合基金重点项目（U1903205）

Selection and in vitro Probiotic Properties of Bifidobacterium longum subsp. longum with Anti-diabetic Activity from the Intestinal Tract of Chinese Tajik Children

LI Si, AN Meiling, LI Longlong, ZHANG Ruirui, SUN Hailong, NI Yongqing

(1. Key Laboratory for Xinjiang Special Probiotics and Dairy Technology of the Eighth Division of Xinjiang Production and Construction Corps, School of Food Science and Technology, Shihezi University, Shihezi 832003, China; 2. Xinjiang Production and Construction Corps Industrial Innovation Research Institute of Dairy Products, Xinjiang Tianrun Biotechnology Co. Ltd., ürümqi 830000, China; 3. Xinjiang Shihezi Huayuan Dairy Co. Ltd., Shihezi 832003, China)

Online:2025-05-25 Published:2025-05-07

摘要/Abstract

摘要： 益生菌在预防和治疗糖尿病方面已被证实具有积极作用。不同人群的肠道菌群蕴含了极其丰富的物种和菌株多样性，本研究旨在探索塔吉克族儿童肠道中的潜在新益生菌资源，以期发现可用于糖尿病防治的新菌株。从新疆塔吉克族健康儿童肠道中分离双歧杆菌菌株，并采用特异的groEL基因测序技术对菌株进行鉴定，结合其体外益生特性筛选潜在的抗糖尿病菌株。结果显示，从62 名儿童肠道中共分离纯化出1 579 株双歧杆菌，其中长双歧杆菌长亚种（Bifidobacterium longum subsp. longum）为优势菌株（591 株）。从33 个分离出长双歧杆菌的不同样本中，每份样本至少挑选1 株已测序的长双歧杆菌长亚种菌株（共76 株）进行体外益生特性分析。其中长双歧杆菌长亚种TXX165-6、TXX127-13、TXX13-17、TXX51-1和TXY63-27在模拟胃肠液中的存活率均超过60%，并具有良好的碳源利用能力，其无细胞上清液具有良好的抗氧化活性和α-葡萄糖苷酶抑制活性，对2,2’-联氮-双-(3-乙基苯并噻唑啉-6-磺酸)阳离子自由基的清除率超过82%，对1,1-二苯基-2-三硝基苯肼自由基的清除率均在68%以上。菌株TXX165-6的表面疏水性（78.46%）和表面自聚性（49.15%）最强。菌株TXX51-1表面疏水性（67.30%）次之，但对α-葡萄糖苷酶抑制率高达68.75%，明显高于对照组模式益生菌菌株鼠李糖乳酪杆菌GG（20.15%）。因此，长双歧杆菌长亚种TXX165-6和TXX51-1可作为潜在干预糖尿病作用菌株进一步用于动物实验。

关键词: 长双歧杆菌长亚种；抗氧化；α-葡萄糖苷酶抑制活性；益生特性

Abstract: Probiotics have been proven to play a positive role in the prevention and treatment of diabetes. The gut microbiota of different populations harbors an extremely rich diversity of species and strains. This study aimed to explore potential new probiotic resources from the intestine of Tajik children with the goal of discovering new strains that can be used for the prevention and treatment of diabetes. In this study, Bifidobacterium strains were isolated from the intestine of healthy Tajik children in Xinjiang and identified using groEL gene sequencing. These strains were then screened for their in vitro probiotic characteristics to identify potential anti-diabetic strains. The results showed that 1 579 Bifidobacterium strains were isolated and purified from the intestine of 62 children, with Bifidobacterium longum subsp. longum being the dominant ones (591 isolates). A total of 76 sequenced strains of B. longum subsp. longum from 33 samples containing B. longum, at least one strain of which was from each sample, were analyzed for in vitro probiotic characteristics. The results showed that five B. longum subsp. longum strains (TXX165-6, TXX127-13, TXX13-17, TXX51-1, and TXY63-27) exhibited a survival rate exceeding 60% in simulated gastrointestinal fluid and demonstrated good carbon source utilization capabilities. All of their cell-free supernatants exhibited strong antioxidant activity and α-glucosidase inhibitory activity, scavenging over 82% of 2,2’-azino-bis-(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) cation radical and over 68% of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical. Strain TXX165-6 exhibited the highest surface hydrophobicity (78.46%) and surface self-aggregation ability (49.15%). Strain TXX51-1 displayed the second-highest surface hydrophobicity (67.30%) and inhibited 68.75% of α-glucosidase activity, which was significantly superior to the model probiotic Lacticaseibacillus rhamnosus GG (20.15%). Therefore, strains TXX165-6 and TXX51-1 are promising candidates for further animal experiments to assess their potential as potential anti-diabetic strains.

Key words: Bifidobacterium longum subsp. longum; antioxidant activity; α-glucosidase inhibitory activity; probiotic properties

中图分类号:

Q938

李思，安美玲，李珑珑，张瑞瑞，孙海龙，倪永清. 我国塔吉克族儿童肠道拮抗糖尿病长双歧杆菌长亚种优势菌株筛选及其体外益生特性[J]. 食品科学, 2025, 46(10): 128-138.

LI Si, AN Meiling, LI Longlong, ZHANG Ruirui, SUN Hailong, NI Yongqing. Selection and in vitro Probiotic Properties of Bifidobacterium longum subsp. longum with Anti-diabetic Activity from the Intestinal Tract of Chinese Tajik Children[J]. FOOD SCIENCE, 2025, 46(10): 128-138.

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我国塔吉克族儿童肠道拮抗糖尿病长双歧杆菌长亚种优势菌株筛选及其体外益生特性

Selection and in vitro Probiotic Properties of Bifidobacterium longum subsp. longum with Anti-diabetic Activity from the Intestinal Tract of Chinese Tajik Children

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