QuEChERS法同时测定水产品中邻苯二甲酸酯及其代谢物的含量

doi:10.7506/spkx1002-6630-20241224-191

食品科学 ›› 2025, Vol. 46 ›› Issue (13): 365-339.doi: 10.7506/spkx1002-6630-20241224-191

• 安全检测 • 上一篇

QuEChERS法同时测定水产品中邻苯二甲酸酯及其代谢物的含量

林伟健，张艺严，黄育坊，王姣姣，李敏倩，吕辉雄，黄晓梅

（1.华南农业大学资源环境学院，广东广州 510642；2.广东省农业科学院农业质量标准与监测技术研究所，广东广州 510640；3.华南农业大学海洋学院，广东广州 510642；4.广东省农产品质量安全风险评估重点实验室，广东广州 510640；5.猪禽种业全国重点实验室，广东广州 510640）

发布日期:2025-06-13
基金资助:
广东省基础与应用基础研究基金项目（2024A1515030031；2023A1515012091）；广州市基础与应用基础项目（2023A04J0785；2023A04J0782）；广东省农业科学院猪禽种业全国重点实验室2023年度自主科研项目（2023QZ-NK15）

QuEChERS Method for Simultaneous Determination of Phthalates and Their Metabolites in Aquatic Products

LIN Weijian, ZHANG Yiyan, HUANG Yufang, WANG Jiaojiao, LI Minqian, LÜ Huixiong, HUANG Xiaomei

(1. College of Natural Resources and Environment, South China Agricultural University, Guangzhou 510642, China; 2. Institute of Quality Standard and Monitoring Technology for Agro-products, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China; 3. College of Marine Sciences, South China Agricultural University, Guangzhou 510642, China; 4. Guangdong Provincial Key Laboratory of Quality & Safety Risk Assessment for Agro-products, Guangzhou 510640, China; 5. State Key Laboratory of Swine and Poultry Breeding Industry, Guangzhou 510640, China)

Published:2025-06-13

摘要/Abstract

摘要： 基于QuEChERS法对水产品中19 种邻苯二甲酸酯（phthalate esters，PAEs）和9 种邻苯二甲酸单酯（phthalate monoesters，mPAEs）进行同时前处理后，采用超高效液相色谱-高分辨质谱法测定其PAEs和mPAEs的含量。结果显示，19 种PAEs和9 种mPAEs的基质加标平均回收率分别为71%～120%和87%～119%，方法检出限分别为1～48 ng/g（以干质量计，下同）和1～6 ng/g。值得注意的是，本研究通过优化色谱条件，实现了4 组PAEs和1 组mPAEs同分异构体的基线分离，尤其是邻苯二甲酸二丁酯（dibutyl phthalate，DBP）与邻苯二甲酸二异丁酯（diisobutyl phthalate，DIBP）、邻苯二甲酸单丁酯（monobutyl phthalate，MBP）与邻苯二甲酸单异丁酯（monoisobutyl phthalate，MIBP）。本方法应用于广州市17 种市售水产品，结果显示，水产品中DBP、DIBP、邻苯二甲酸二(2-乙基己基)酯、邻苯二甲酸(4-甲基-2-戊基)酯、邻苯二甲酸丁苄酯、邻苯二甲酸二正戊酯6 种PAEs和MBP、MIBP、邻苯二甲酸单(2-乙基己基)酯3 种mPAEs的检出率较高。该方法操作简洁且灵敏度高，将为水产品中PAEs和mPAEs的检测和风险评估提供重要技术支撑。

关键词: 邻苯二甲酸酯；邻苯二甲酸单酯；超高效液相色谱-高分辨质谱法；水产品

Abstract: This study developed a method for the simultaneous determination of 19 phthalate esters (PAEs) and 9 phthalate monoesters (mPAEs) in aquatic products using ultra-high performance liquid chromatography-high-resolution mass spectrometry (UPLC-HRMS) after quick, easy, cheap, efficient, rugged and safe (QuEChERS) pretreatment. The results indicated that the average recoveries for 19 PAEs and 9 mPAEs in spiked matrices were 71%–120% and 87%–119%, with detection limits of 1–48 and 1–6 ng/g md, respectively. It was noted that under optimized chromatographic conditions, baseline separation of the isomers of four PAEs and one mPAE was achieved, especially diisobutyl phthalate (DIBP) versus dibutyl phthalate (DBP), and monoisobutyl phthalate (MIBP) versus monobutyl phthalate (MBP). This method was applied to 17 commercially available aquatic products in Guangzhou, and the results showed that the detection rates of six PAEs, including DBP, DIBP, di(2-ethylhexyl) phthalate, bis(4-methyl-2-pentyl) phthalate, butyl benzyl phthalate, di-n-pentyl phthalate, as well as three mPAEs, including MBP, MIBP, and mono(2-ethylhexyl) phthalate, were relatively high. This method is simple to operate, with high sensitivity, providing important technical support for the detection and risk assessment of PAEs and mPAEs in aquatic products.

Key words: phthalate esters; phthalate monoester; ultra-high performance liquid chromatography-high-resolution mass spectrometry; aquatic products

中图分类号:

O657.63

林伟健，张艺严，黄育坊，王姣姣，李敏倩，吕辉雄，黄晓梅. QuEChERS法同时测定水产品中邻苯二甲酸酯及其代谢物的含量[J]. 食品科学, 2025, 46(13): 365-339.

LIN Weijian, ZHANG Yiyan, HUANG Yufang, WANG Jiaojiao, LI Minqian, LÜ Huixiong, HUANG Xiaomei. QuEChERS Method for Simultaneous Determination of Phthalates and Their Metabolites in Aquatic Products[J]. FOOD SCIENCE, 2025, 46(13): 365-339.

[1]	刘际通，王新怡，张一敏，朱立贤，梁荣蓉，毛衍伟. 基于蛋白质组学分析过氧化物还原酶6的非钙依赖型磷脂酶A2活性调控牛肉嫩化机制[J]. 食品科学, 2025, 46(11): 20-28.
[2]	周文静，王卢燕，周昌瑜，方楠，张昌朋，蒋金花. QuEChERS-超高效液相色谱-串联质谱法同时测定果蔬中新型甲氧基丙烯酸酯类杀菌剂Mandestrobin和Metyltetraprole残留[J]. 食品科学, 2025, 46(10): 274-281.
[3]	吴建欣，王宗义，于淼，毕桂宾，张巍青，董子琦，马小童. 挥发性离子对反相液相色谱-串联质谱法检测冷链鱼虾中16 种生物胺[J]. 食品科学, 2025, 46(10): 282-289.
[4]	冯峰，常启智，孙晓，杜容竹，张峰. 超高效液相色谱-高分辨质谱法快速测定食品中107种那非类非法添加物[J]. 食品科学, 2025, 46(10): 290-298.
[5]	曾涛，杨荣，李晓晶，甘平胜，刘苗，卢祝靓子，李燕，白志军. 预制菜中16 种邻苯二甲酸酯类塑化剂的测定方法研究及污染水平分析[J]. 食品科学, 2025, 46(9): 329-336.
[6]	张海超，魏欣欣，张婧雯，贾海涛，艾连峰，王敬，黄雪静，秦晓航，刘宝如. 超高效液相色谱-大气压化学电离-串联质谱法快速测定禽蛋中地克珠利和妥曲珠利残留标识物[J]. 食品科学, 2025, 46(8): 330-339.
[7]	孙荷芝戴中洋马进森王新茹罗宗秀修春丽周利. 苯醚甲环唑在茉莉花茶上的转移行为和摄入风险[J]. 食品科学, 0, (): 0-0.
[8]	王娜，沈毅，庄园，程伟，罗森，张亚东，刘子轩，刘冰，高红波. 气相色谱-串联质谱同时测定白酒中20 种吡嗪类化合物[J]. 食品科学, 2025, 46(5): 30-37.
[9]	杨修，严艳芳，李齐激，杨礼寿，潘雄，李立郎，胡薛豪，陈瑞，罗夫来，高明，杨小生. 基于高效液相色谱指纹图谱的仿野生天麻品质及抗氧化和神经保护活性[J]. 食品科学, 2025, 46(5): 226-235.
[10]	王娜沈毅庄园程伟罗森张亚东刘子轩刘冰高红波. 气相色谱-串联质谱同时测定白酒中20 种吡嗪类化合物 [J]. 食品科学, 0, (): 0-0.
[11]	薛霞魏莉莉王骏宿书芳公丕学张艳侠倪来学姚现琦刘艳明万宇平. 亲水作用色谱-串联质谱法检测畜禽肝脏和肾脏中氨基糖苷类药物残留[J]. 食品科学, 0, (): 0-0.
[12]	刘佳乐付湘晋郑兰萍刘颖刘敏陈亚泉龙肇. 不同产地虾酱代谢物组比较研究[J]. 食品科学, 0, (): 0-0.
[13]	屠大伟，马宏能，李佳，康峻菡，刘文俊. 川渝地区豆瓣的常规理化和挥发性风味物质及细菌群落分析[J]. 食品科学, 2025, 46(3): 187-195.
[14]	叶新玉，李子樱，唐淑军，孙丽萍，赵云霞，肖伟敏，顾亚萍，匡慧颖，陈欣，杨国武，黄雅丽. 果蔬中天冬酰胺和谷氨酰胺含量检测方法及尿素循环障碍患者食用分析[J]. 食品科学, 2025, 46(3): 212-221.
[15]	何金麟，沈鹭英，孙婷，傅建炜，严丽娟，徐敦明. 弱阳离子交换固相萃取-超高效液相色谱-串联质谱法测定牛奶中13 种氨基糖苷类药物残留[J]. 食品科学, 2025, 46(3): 238-245.

QuEChERS法同时测定水产品中邻苯二甲酸酯及其代谢物的含量

QuEChERS Method for Simultaneous Determination of Phthalates and Their Metabolites in Aquatic Products

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价