产MBLs奶牛源致病性大肠杆菌O157:H12菌株的分子特性和致病性分析

doi:10.7506/spkx1002-6630-20250822-164

食品科学 ›› 2026, Vol. 47 ›› Issue (6): 155-166.doi: 10.7506/spkx1002-6630-20250822-164

• 生物工程 • 上一篇

产MBLs奶牛源致病性大肠杆菌O157:H12菌株的分子特性和致病性分析

张哲，袁紫婧，张启文，吴利军，张付贤

（1.长江大学动物科学技术学院，湖北荆州 434023；2.武汉市农业科学院畜牧兽医研究所，湖北武汉 430208）

发布日期:2026-04-14
基金资助:
湖北省重大科技专项（2024BBA004）；荆州市科技计划项目（2025CC32）；荆州市揭榜制科技项目（2025JA10）

Molecular Characterization and Pathogenicity Analysis of MBLs-Producing Pathogenic Escherichia coli O157:H12 Strains from Dairy Cows

ZHANG Zhe, YUAN Zijing, ZHANG Qiwen, WU Lijun, ZHANG Fuxian

(1. College of Animal Science and Technology, Yangtze University, Jingzhou 434023, China; 2. Institute of Animal Husbandry and Veterinary, Wuhan Academy of Agricultural Sciences, Wuhan 430208, China)

Published:2026-04-14

摘要/Abstract

摘要： 为探究湖北某奶牛场犊牛腹泻病因，从奶牛场采集粪便、肛拭子和牛奶样品，通过病原筛查和分离鉴定，进一步采用聚合酶链式反应（polymerase chain reaction，PCR）鉴定、多位点序列分型（multi-locus sequence typing，MLST）分析等方法对分离菌株进行分子分型鉴定，并进行致病性和耐药性分析。结果表明，16 份肛拭子、21 份粪便和12 份牛奶样本中分离出4 株优势菌株，经16S rRNA和PCR鉴定后，其中3 株菌为同一株大肠杆菌O157（Escherichia coli O157），命名为JZ-0720；MLST分析确定分离菌株为ST10型；血清学和PCR鉴定确定分离菌株为E. coli O157:H12型。分离菌株JZ-0720携带XJrfbE、uspA和stx1 3 种毒力基因，对Vero细胞能产生类似E. coli O157:H7的毒素效果；小鼠感染分离菌株JZ-0720后多个器官出现不同程度的病变，低剂量感染则导致小鼠腹泻，高剂量感染可致小鼠死亡，其半数致死剂量为5.36×108 CFU/mL；分离菌株JZ-0720携带gyrA、bla-CTX、bla-TEM、bla-NDM和aadA1 5 种耐药基因，对新霉素、红霉素等药物耐药；双协同药敏实验显示，分离菌株产金属β-内酰胺酶（metallo-β-lactamases，MBLs）而不产超广谱β-内酰胺酶；中药乌梅、艾叶对JZ-0720有很好的抑菌效果。综上，本研究分离得到1 株血清型为E. coli O157:H12型的奶牛源致病性大肠杆菌且MLST分型为ST10型，携带多种毒力基因和耐药基因，进行人工感染可致小鼠腹泻，对中药乌梅、艾叶以及多种抗生素敏感。研究结果可为E. coli O157非H7型大肠杆菌的防治研究提供参考依据。

关键词: 大肠杆菌O157:H12；分离鉴定；致病性；耐药性

Abstract: To investigate the causes of calf diarrhea on a dairy farm in Hubei Province, fecal samples, rectal swabs, and milk samples were collected from the dairy farm. After pathogen screening, isolation, and identification, the isolated strains were further characterized using polymerase chain reaction (PCR) and multilocus sequence typing (MLST). Additionally, their pathogenicity and drug resistance were analyzed. The results showed that 4 dominant strains were isolated from 16 anal swabs, 21 fecal samples, and 12 milk samples. Following 16S rRNA and PCR identification, 3 strains were identified as Escherichia coli O157 and designated as JZ-0720. MLST analysis determined the isolates belonged to sequence type ST10. Serological and PCR identification confirmed the isolates as serotype O157:H12. The isolate JZ-0720 carried three virulence genes (XJrfbE, uspA, and stx1) and produced a toxic effect on Vero cells similar to that of O157:H7. Infection of mice with JZ-0720 caused varying degrees of pathological lesions in multiple organs. Low-dose infection induced diarrhea in mice, while high-dose infection was lethal, with a median lethal dose (LD50) of 5.36 × 108 CFU/mL. The isolate JZ-0720 carried five drug resistance genes (gyrA, bla-CTX, bla-TEM, bla-NDM, and aadA1) and exhibited resistance to neomycin and erythromycin. The double-disk synergy test indicated the strain produced metallo-β-lactamases (MBLs) but not extended-spectrum β-lactamases (ESBLs). The traditional Chinese medicines Mume Fructus and Artemisiae Argyi Folium demonstrated significant inhibitory effects against JZ-0720. The results from this research provide a basis for the prevention and control of non-H7 serotypes of E. coli O157.

Key words: Escherichia coli O157:H12; isolation and identification; pathogenicity; drug resistance

中图分类号:

S823.9＋1

张哲，袁紫婧，张启文，吴利军，张付贤. 产MBLs奶牛源致病性大肠杆菌O157:H12菌株的分子特性和致病性分析[J]. 食品科学, 2026, 47(6): 155-166.

ZHANG Zhe, YUAN Zijing, ZHANG Qiwen, WU Lijun, ZHANG Fuxian. Molecular Characterization and Pathogenicity Analysis of MBLs-Producing Pathogenic Escherichia coli O157:H12 Strains from Dairy Cows[J]. FOOD SCIENCE, 2026, 47(6): 155-166.

[1]	张哲袁紫婧张启文吴利军张付贤. 产MBLs奶牛源致病性大肠杆菌O157：H12菌株的分子特性和致病性分析[J]. 食品科学, 0, (): 0-0.
[2]	和占星，黄梅芬，赵刚，王向东，成玉梅，张继才，杨凯，王安奎. 不同品种牛乳冰点及其与乳理化指标相关性分析[J]. 食品科学, 2017, 38(17): 94-100.

产MBLs奶牛源致病性大肠杆菌O157:H12菌株的分子特性和致病性分析

Molecular Characterization and Pathogenicity Analysis of MBLs-Producing Pathogenic Escherichia coli O157:H12 Strains from Dairy Cows

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 2

编辑推荐

Metrics

本文评价