乳桥蛋白的动态模拟婴幼儿胃肠消化及其产物钙螯合能力与促肠吸收活性

doi:10.7506/spkx1002-6630-20251219-159

食品科学 ›› 2026, Vol. 47 ›› Issue (11): 151-161.doi: 10.7506/spkx1002-6630-20251219-159

乳桥蛋白的动态模拟婴幼儿胃肠消化及其产物钙螯合能力与促肠吸收活性

赵炫湘，黄潘钿，曹文俊，陈凯特，刘斐童，胡瑞标，苗建银

（1.华南农业大学食品学院，广东省功能食品活性物重点实验室，广东广州 510642；2.合生元（长沙）营养食品有限公司，湖南长沙 410219；3.健合（中国）有限公司，广东广州 510700）

发布日期:2026-07-02
基金资助:
广东省省级科技计划项目（2025A0505020075）；广东省自然科学基金面上项目（2023A1515010006）

Dynamic Simulation of Infant Gastrointestinal Digestion of Lactopontin: Calcium Chelation Capacity and Intestinal Absorption-Promoting Activity of Its Hydrolysates

ZHAO Xuanxiang, HUANG Pantian, CAO Wenjun, CHEN Kaite, LIU Feitong, HU Ruibiao, MIAO Jianyin

(1. Guangdong Provincial Key Laboratory of Nutraceuticals and Functional Foods, College of Food Science, South China Agricultural University, Guangzhou 510642, China; 2. Biostime (Changsha) Nutrition Foods Limited, Changsha 410219, China; 3. Health and Happiness (H&H) China Limited, Guangzhou 510700, China)

Published:2026-07-02

摘要/Abstract

摘要： 采用体外动态胃肠消化系统，模拟1～3 岁婴幼儿胃肠生理环境对乳桥蛋白（lactopontin，L-OPN）进行消化处理，脱盐后制备得到乳桥蛋白肽（lactopontin peptide，L-OPN-P），并系统探究其钙螯合特性及肠道钙转运促进活性。结果表明：消化产物L-OPN-P在模拟肠道环境下展现出优异的钙螯合能力，与钙离子结合形成乳桥蛋白肽钙螯合物（lactopontin peptide-chelated calcium，L-OPN-P-Ca）后，其粒径与Zeta电位较L-OPN-P显著增大，证实二者成功形成新型螯合产物；氨基酸组成分析显示，L-OPN-P-Ca中天冬氨酸、苏氨酸、丝氨酸及谷氨酸的相对含量较L-OPN-P显著升高；紫外光谱与傅里叶变换红外光谱分析揭示，钙离子通过与L-OPN-P分子中的羧基氧、氨基氮发生配位作用形成稳定螯合物，扫描电镜观察结果进一步验证了该螯合反应的发生。在Caco-2细胞模型模拟小肠钙转运实验中，L-OPN-P与L-OPN-P-Ca在180 min时的钙转运量分别达（54.10±0.33）µg/well和（62.93±3.03）µg/well，均表现出突出的促肠钙吸收活性，且L-OPN-P-Ca的促进效果更为显著。本研究为L-OPN在婴幼儿配方奶粉中的应用开发以及新型高效婴幼儿补钙产品的研制提供了重要理论依据与创新方向。

关键词: 乳桥蛋白；体外动态消化；乳桥蛋白肽；肽钙螯合物；表征；促钙吸收活性

Abstract: This study prepared lactopontin-derived peptides (L-OPN-P) by digesting lactopontin (L-OPN) in a dynamic in vitro digestion system simulating the gastrointestinal environment of 1 to 3-year-old infants, followed by desalting. The calcium-chelating capacity and intestinal calcium transport-promoting activity of L-OPN-P were systematically investigated. Results indicated that under simulated intestinal conditions, L-OPN-P exhibited excellent calcium-chelating capacity. The resulting chelate lactopontin peptide-chelated calcium (L-OPN-P-Ca) exhibited obviously increased particle size and zeta potential compared with L-OPN-P. Amino acid analysis revealed that the relative contents of aspartic acid (Asp), threonine (Thr), serine (Ser), and glutamic acid (Glu) in L-OPN-P-Ca were significantly increased compared with L-OPN-P. Ultraviolet (UV) and Fourier transform infrared spectroscopy (FTIR) revealed that calcium ions formed stable chelates with L-OPN-P through coordination with carboxyl oxygen and amino nitrogen atoms. Scanning electron microscopy (SEM) observations provided further confirmation of this chelation reaction. In a Caco-2 cell model simulating intestinal calcium transport, the amounts of calcium transported by L-OPN-P and L-OPN-P-Ca at 180 min reached (54.10 ± 0.33) and (62.93 ± 3.03) µg/well, respectively, indicating that both exhibited prominent intestinal calcium absorption-promoting activity, and the promoting effect of L-OPN-P-Ca was more significant. This study provides an important theoretical basis and innovative directions for the application of L-OPN in infant formula, as well as the development of novel efficient calcium supplements for infants.

Key words: lactopontin; in vitro dynamic digestion; lactopontin-derived peptides; peptide-calcium chelate; characterization; calcium absorption-promoting activity

中图分类号:

TS201.4

赵炫湘，黄潘钿，曹文俊，陈凯特，刘斐童，胡瑞标，苗建银. 乳桥蛋白的动态模拟婴幼儿胃肠消化及其产物钙螯合能力与促肠吸收活性[J]. 食品科学, 2026, 47(11): 151-161 https://doi.org/10.7506/spkx1002-6630-20251219-159

ZHAO Xuanxiang, HUANG Pantian, CAO Wenjun, CHEN Kaite, LIU Feitong, HU Ruibiao, MIAO Jianyin. Dynamic Simulation of Infant Gastrointestinal Digestion of Lactopontin: Calcium Chelation Capacity and Intestinal Absorption-Promoting Activity of Its Hydrolysates[J]. FOOD SCIENCE, 2026, 47(11): 151-161 https://doi.org/10.7506/spkx1002-6630-20251219-159

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乳桥蛋白的动态模拟婴幼儿胃肠消化及其产物钙螯合能力与促肠吸收活性

Dynamic Simulation of Infant Gastrointestinal Digestion of Lactopontin: Calcium Chelation Capacity and Intestinal Absorption-Promoting Activity of Its Hydrolysates

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