乳桥蛋白的动态模拟婴幼儿胃肠消化及其产物钙螯合能力与促肠吸收活性

doi:10.7506/spkx1002-6630-20251219-159

食品科学 ›› 2026, Vol. 47 ›› Issue (11): 151-161.doi: 10.7506/spkx1002-6630-20251219-159

• 营养卫生 • 上一篇

乳桥蛋白的动态模拟婴幼儿胃肠消化及其产物钙螯合能力与促肠吸收活性

赵炫湘，黄潘钿，曹文俊，陈凯特，刘斐童，胡瑞标，苗建银

（1.华南农业大学食品学院，广东省功能食品活性物重点实验室，广东广州 510642；2.合生元（长沙）营养食品有限公司，湖南长沙 410219；3.健合（中国）有限公司，广东广州 510700）

发布日期:2026-07-02
基金资助:
广东省省级科技计划项目（2025A0505020075）；广东省自然科学基金面上项目（2023A1515010006）

Dynamic Simulation of Infant Gastrointestinal Digestion of Lactopontin: Calcium Chelation Capacity and Intestinal Absorption-Promoting Activity of Its Hydrolysates

ZHAO Xuanxiang, HUANG Pantian, CAO Wenjun, CHEN Kaite, LIU Feitong, HU Ruibiao, MIAO Jianyin

(1. Guangdong Provincial Key Laboratory of Nutraceuticals and Functional Foods, College of Food Science, South China Agricultural University, Guangzhou 510642, China; 2. Biostime (Changsha) Nutrition Foods Limited, Changsha 410219, China; 3. Health and Happiness (H&H) China Limited, Guangzhou 510700, China)

Published:2026-07-02

摘要/Abstract

摘要： 采用体外动态胃肠消化系统，模拟1～3 岁婴幼儿胃肠生理环境对乳桥蛋白（lactopontin，L-OPN）进行消化处理，脱盐后制备得到乳桥蛋白肽（lactopontin peptide，L-OPN-P），并系统探究其钙螯合特性及肠道钙转运促进活性。结果表明：消化产物L-OPN-P在模拟肠道环境下展现出优异的钙螯合能力，与钙离子结合形成乳桥蛋白肽钙螯合物（lactopontin peptide-chelated calcium，L-OPN-P-Ca）后，其粒径与Zeta电位较L-OPN-P显著增大，证实二者成功形成新型螯合产物；氨基酸组成分析显示，L-OPN-P-Ca中天冬氨酸、苏氨酸、丝氨酸及谷氨酸的相对含量较L-OPN-P显著升高；紫外光谱与傅里叶变换红外光谱分析揭示，钙离子通过与L-OPN-P分子中的羧基氧、氨基氮发生配位作用形成稳定螯合物，扫描电镜观察结果进一步验证了该螯合反应的发生。在Caco-2细胞模型模拟小肠钙转运实验中，L-OPN-P与L-OPN-P-Ca在180 min时的钙转运量分别达（54.10±0.33）µg/well和（62.93±3.03）µg/well，均表现出突出的促肠钙吸收活性，且L-OPN-P-Ca的促进效果更为显著。本研究为L-OPN在婴幼儿配方奶粉中的应用开发以及新型高效婴幼儿补钙产品的研制提供了重要理论依据与创新方向。

关键词: 乳桥蛋白；体外动态消化；乳桥蛋白肽；肽钙螯合物；表征；促钙吸收活性

Abstract: This study prepared lactopontin-derived peptides (L-OPN-P) by digesting lactopontin (L-OPN) in a dynamic in vitro digestion system simulating the gastrointestinal environment of 1 to 3-year-old infants, followed by desalting. The calcium-chelating capacity and intestinal calcium transport-promoting activity of L-OPN-P were systematically investigated. Results indicated that under simulated intestinal conditions, L-OPN-P exhibited excellent calcium-chelating capacity. The resulting chelate lactopontin peptide-chelated calcium (L-OPN-P-Ca) exhibited obviously increased particle size and zeta potential compared with L-OPN-P. Amino acid analysis revealed that the relative contents of aspartic acid (Asp), threonine (Thr), serine (Ser), and glutamic acid (Glu) in L-OPN-P-Ca were significantly increased compared with L-OPN-P. Ultraviolet (UV) and Fourier transform infrared spectroscopy (FTIR) revealed that calcium ions formed stable chelates with L-OPN-P through coordination with carboxyl oxygen and amino nitrogen atoms. Scanning electron microscopy (SEM) observations provided further confirmation of this chelation reaction. In a Caco-2 cell model simulating intestinal calcium transport, the amounts of calcium transported by L-OPN-P and L-OPN-P-Ca at 180 min reached (54.10 ± 0.33) and (62.93 ± 3.03) µg/well, respectively, indicating that both exhibited prominent intestinal calcium absorption-promoting activity, and the promoting effect of L-OPN-P-Ca was more significant. This study provides an important theoretical basis and innovative directions for the application of L-OPN in infant formula, as well as the development of novel efficient calcium supplements for infants.

Key words: lactopontin; in vitro dynamic digestion; lactopontin-derived peptides; peptide-calcium chelate; characterization; calcium absorption-promoting activity

中图分类号:

TS201.4

赵炫湘，黄潘钿，曹文俊，陈凯特，刘斐童，胡瑞标，苗建银. 乳桥蛋白的动态模拟婴幼儿胃肠消化及其产物钙螯合能力与促肠吸收活性[J]. 食品科学, 2026, 47(11): 151-161 https://doi.org/10.7506/spkx1002-6630-20251219-159

ZHAO Xuanxiang, HUANG Pantian, CAO Wenjun, CHEN Kaite, LIU Feitong, HU Ruibiao, MIAO Jianyin. Dynamic Simulation of Infant Gastrointestinal Digestion of Lactopontin: Calcium Chelation Capacity and Intestinal Absorption-Promoting Activity of Its Hydrolysates[J]. FOOD SCIENCE, 2026, 47(11): 151-161 https://doi.org/10.7506/spkx1002-6630-20251219-159

[1]	余海立郑明山张彦陈智仙. 酵母蛋白与乳清蛋白对大鼠血浆氨基酸动力学的比较分析[J]. 食品科学, 0, (): 0-0.
[2]	付雨雨朱振宝梁蕾顾浩峰韦阳王自超曾翠艳邓之豪麻婉宁王佳豪南鑫胡佳雪高万祥. 茶叶α-葡萄糖苷酶抑制肽的制备工艺及其抑制机制[J]. 食品科学, 0, (): 0-0.
[3]	牟晓娟，曾琦琪，穆合塔尔・斯地克，刘宇恒，李凤鸣，吴同华，徐均，张军，包晓玮. H2O2-VC降解对沙棘果皮渣多糖初级结构及体外抗氧化与降糖活性的影响[J]. 食品科学, 0, (): 162-182.
[4]	祝辉，胡虹君，马懿，蒋维明，沈才洪，敖宗华，宋川，熊蓉，蒋松樾，刘渠波，泽仁磋，徐柯伟，王凝. 藏茶茶褐素通过调控IRE1/p-JNK/Bcl-2-Caspase-3通路在阿尔茨海默病斑马鱼模型中神经保护作用的机制[J]. 食品科学, 0, (): 183-212.
[5]	董玮琪，谢金晖，崔添玉，辛嘉英. 聚羟基丁酸酯的益生元作用机制与发酵生产优化及其在食品中的应用[J]. 食品科学, 0, (): 413-436.
[6]	邹畅万茜张兴静刘权康褚忠兴曹福亮周亚萍罗非君. 基于网络药理学与16S rRNA技术探究二十八烷醇抗疲劳作用及肠道微生态调控机制[J]. 食品科学, 0, (): 0-0.
[7]	黄小露郭美玲周姝芯武运杜木英. 基于代谢组学揭示巫山庙党热泵干燥过程中的化学变化[J]. 食品科学, 0, (): 0-0.
[8]	谢瑶刘静邓逸凡范春雪辛念徐敏. 黄芪香薷酵母发酵制剂对微重力环境下大鼠免疫功能及肠道稳态的影响[J]. 食品科学, 0, (): 0-0.
[9]	张放徐皓男孙婷婷车燕曹伟亮陈浩. 羊肚菌多糖与抗生素协同改善结肠炎及其肝肠轴机制[J]. 食品科学, 0, (): 0-0.
[10]	张亿张兴张超熊子奕李欣陈红兵. 山羊乳酪蛋白体外动态消化肽通过调节肠道菌群缓解溃疡性结肠炎[J]. 食品科学, 0, (): 0-0.
[11]	宋陆清花朋朋闫巧娟江正强鲁绯. 罗非鱼鳞胶原蛋白肽对高脂血症小鼠的脂代谢调节效果及作用机制[J]. 食品科学, 0, (): 0-0.
[12]	张安东张川张程程黄攀赵建新翟齐啸陈卫田丰伟. 体外模拟肠道消化发酵体系中阿胶组分对肠道菌群结构的影响[J]. 食品科学, 0, (): 0-0.
[13]	王微王倩倩黄婷李泽惠闵伟红. 核桃源缓解便秘活性肽的筛选及功能验证[J]. 食品科学, 0, (): 0-0.
[14]	杨蕊羽，李鹤宾，朱远翔，洪涛，郑明静，姜泽东，倪辉，朱艳冰. 海藻酸钠对黄嘌呤氧化酶活性的抑制作用及其抑制机制[J]. 食品科学, 2026, 47(10): 55-62.
[15]	於钊庆，范文卓，梁清，聂梅梅，肖亚冬，戴竹青，李大婧. 西梅果渣多酚纯化、成分鉴定及体外降血糖活性分析[J]. 食品科学, 2026, 47(10): 63-74.

乳桥蛋白的动态模拟婴幼儿胃肠消化及其产物钙螯合能力与促肠吸收活性

Dynamic Simulation of Infant Gastrointestinal Digestion of Lactopontin: Calcium Chelation Capacity and Intestinal Absorption-Promoting Activity of Its Hydrolysates

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价