食品科学 ›› 1993, Vol. 14 ›› Issue (8): 61-63.

• 食品科学 • 上一篇    下一篇

反相高效液相色谱法测定草莓中的维生素C

王卫东   

  1. 中国科学院长沙农业现代化研究所
  • 出版日期:1993-08-15 发布日期:2011-12-30

A Method for the Determination of Vitamin C in Strawberry by Reversed -Phase High Performance Liquid Chromatography

 WANG  Wei-Dong   

  • Online:1993-08-15 Published:2011-12-30

摘要: 报道了测定草莓中维生素C的反相高效液相色谱法。样品中的维生索C在zorbax ODs(5μm)反相色谱柱上分离、以0.025mol/LKH2PO4—甲醇(70:30)为流动相洗脱、用紫外检测法检测(λ=254 nm)。维生素C在2.5min内得到了良好的分离,检测范围为025mg/L。用于实际样品的分析,效果良好。

关键词: 反相高效液粉色谱, 草莓, 维生素C

Abstract:  A method for the determination of vitamin c in strawberry by reversed-phase high performance liquid chromatography has been reported in this paper. Vitamin c was separated on a Zorbax ODS (5μm) reversed-phase column using a solution of 0. 025 mol/L KH2PO4: methanol = 70:30 as the mobile phase and detected by ultraviolet spec-trophotometric detector at 254 nm. Good separation of Vitamin c was achieved in 2. 5 min . the range of detection is 0 -25 mg/L. The method was used for the analysis of actual samples and good results were obtained.

Key words:  , Reversed-phase High Performance Liquid Chromatography Strawberry Vitamin C0;