食品科学 ›› 2001, Vol. 22 ›› Issue (4): 31-35.

• 基础研究 • 上一篇    下一篇

酪蛋白磷酸肽(CPPs)的纯化研究

 刘剑虹, 庞广昌, 于金华, 张晓玲, 陈庆森, 胡志和   

  1. 天津商学院食品与生物工程系
  • 出版日期:2001-04-15 发布日期:2012-02-16

Studies on the Purification of Casein Phosphopeptides(CPPs)

LIU  Jian-Hong, PANG  Guang-Chang, YU  Jin-Hua, ZHANG  Xiao-Ling, CHEN  Qing-Sen, HU  Zhi-He   

  • Online:2001-04-15 Published:2012-02-16

摘要: 研究了用大孔强碱性阴离子交换树脂提纯酪蛋白磷酸肽( CPPs)的方法。结果表明,树脂 GM201对 CPPs的分离提纯效果较好,最佳洗脱纯化工艺条件为:盐酸浓度 0.2mol/L, NaCl浓度 0.3mol/L,温度 45℃。在上述最佳工艺条件下, CPPs回收率可达 77.7%~ 80%,纯度提高到 N:P=4.7:4.9。

关键词: 酪蛋白磷酸肽(CPPs), 离子交换树脂, 纯化

Abstract: The purification of casein phosphopeptieds (CPPs) with alkali anion- exchanger was studied.The results were as follows:the purifying effects of the GM201 were best,the optimum eluting parameters were:0.2mol/L hydrochloric acid and 0.3mol/L salt elution at 45℃ .On this condition almost 80% CPPs was recovered and the purity of CPPs was up to 4.7~ 4.9 for N∶ P.

Key words: Casein phosphopeptides (CPPs) Anion- exchange chromatography Purification