食品科学 ›› 2001, Vol. 22 ›› Issue (4): 52-54.

• 工艺技术 • 上一篇    下一篇

生物活性肽-酪蛋白磷酸肽(CPP)的研制

 蔡为荣, 薛正莲   

  1. 安徽机电学院生化工程系
  • 出版日期:2001-04-15 发布日期:2012-02-16

Optimal Parameters Study on Processing Casein Phosphopeptide

 CAI  Wei-Rong, XUE  Zheng-Lian   

  • Online:2001-04-15 Published:2012-02-16

摘要: 摸索了 2709碱性蛋白酶水解酪蛋白制备 CPP的最佳工艺条件:底物浓度 10%、用酶量 1500μ /g反应温度 45℃、 pH10.5、反应时间 150min。采用选择沉淀法分离 CPP,在 pH4.5添加 1.1% CaCl 2( w/v)和 50%( v/v)乙醇,室温沉淀 4h,得到 CPP产品的 N/P、得率分别为 6.39%和 13.94%。

关键词: 酪蛋白磷酸肽, 2709碱性蛋白酶, 酪蛋白

Abstract: In this study,alcalase was used for producing CPP by hydrolyzing casein.The optimum conditions:casein concentration 10% ,enzyme usage 1500u/g substrate,temperature 45℃ ,pH10.5 and time 150mins were determined.A selective precipitation procedure was also used to isolate CPP from the hydrolyzate of casein.The N/P ratio and the yield of CPP obtained by adding 1.1% CaCl 2(w/v) and 50% (v/v) alcohol to the hyrolyzate at pH4.5 were 6.39% and 13.94% respectively.

Key words: Casein phosphopeptide 2709 alcalase Casein