食品科学 ›› 2001, Vol. 22 ›› Issue (6): 24-28.

• 基础研究 • 上一篇    下一篇

酵母发酵法分离纯化低聚异麦芽糖的研究

 岳振峰, 张学兵, 彭志英, 徐建祥, 赵谋   

  1. 华南理工大学生物科学与工程研究中心
  • 出版日期:2001-06-15 发布日期:2012-02-16

Study on Purification of Isomaltooligosaccharide by Yeast Fermentation

 YUE  Zhen-Feng, ZHANG  Xue-Bing, PENG  Zhi-Ying, XU  Jian-Xiang, ZHAO  Mou   

  • Online:2001-06-15 Published:2012-02-16

摘要: 酿酒酵母As 2.109经驯化后,可用于发酵法分离纯化低聚异麦芽糖。优化后酵母发酵的最佳条件为:pH4.0,温度30~32℃,低聚异麦芽糖浓度250g/L,酵母膏浓度0.6g/L,CO(NH2)20.6g/L,Fe2(SO4)30.08g/L,MgSO41.2g/L。在此条件下,低聚异麦芽糖经38h发酵后,低聚异麦芽糖纯度由53.8%提高到90.3%,而葡萄糖被完全除去。

关键词: 酿酒酵母As2.109, 低聚异麦芽糖, 分离纯化

Abstract: After taming,Saccharomyces cerevisiae(As2.109)could be used to purify isomaltooligosacchride by its selective fermentation.The optimum conditions for isomaltooligosacchride’s purification were:pH 4.0,temperature 30~32℃,250g/L isomaltooligosacchride,0.6g/L yeast extracts,0.6g/L CO(NH2)2,0.08g/L Fe2(SO4)3,1.2g/L MgSO4.Under the optimum circumstance,after 38 hours’ fermentation,the content of isomaltooligosacchride was purified up to 90.3%from previous 53.8%,while the glucose was completely eliminated.

Key words: Saccharomyces cerevisiae (As2.109) Isomaltooligosacchride Purification