食品科学 ›› 2001, Vol. 22 ›› Issue (9): 19-22.

• 基础研究 •    下一篇

发酵弥猴桃汁中产香酵母的分离、鉴定及生长特性的研究

 李剑芳,  张灏   

  1.  江南大学食品学院
  • 出版日期:2001-09-15 发布日期:2012-02-15

Isolation,Identification and Growth Characteristics of the Aroma- producing Yeast

 LI  Jian-Fang,   Zhang-Hao   

  • Online:2001-09-15 Published:2012-02-15

摘要:  从优质自然发酵猕猴桃汁中分离筛选到一株产香酵母E-45,经鉴定为柠檬形克勒克氏酵母Kloeckeraapicu-lata。研究了该菌在麦芽汁中的生长特性。其最适生长温度为30℃,最低和最高生长温度分别为7℃和36.5℃;最适生长pH为5.4,最低和最高生长pH分别为1.6和9.5;耐11%的酒精度。其生长曲线显示,于最适生长温度和pH下,摇瓶发酵12~24h便可获得满意的种子。

关键词: 猕猴桃汁, 柠檬形克勒克氏酵母, 分离, 鉴定, 生长特性

Abstract:  A strain of E- 45 was isolated from the natural fermented diwifruit juice.It was further identified as Kloeckera apiculata.The growth characteristics of K.apiculata E- 45 in malt extract were studied.The optimum growth temperature was 30℃ ,while the minimum and maximum growth temperatures were 7℃ and 36.5℃ ,respectively.The optimum growth pH value was 5.4,while the minimum and maximum growth pH values were 1.6 and 9.5 ,respectively.The maximum concentration of ethanol permitting growth was 11% v/v.Based on the strdied results of the growth curve,the seed culture could be obtained by shaking flask culture for 12~ 14h at the optimum growth temperature and pH.

Key words:  , K.apiculata Kiwifruit juice Isolation Identification Growth characteristics;