食品科学 ›› 2003, Vol. 24 ›› Issue (9): 106-108.

• 工艺技术 • 上一篇    下一篇

活性炭对枯草芽孢杆菌β-甘露聚糖酶的作用

 余红英, 孙远明, 杨幼慧, 杨跃生   

  1. 华南农业大学食品学院
  • 出版日期:2003-09-15 发布日期:2011-12-13

Study on Effects of Five Carbon to Decolor β-mannanase of Bacillus Subtilis

 YU  Hong-Ying, SUN  Yuan-Ming, YANG  You-Hui, YANG  Yue-Sheng   

  • Online:2003-09-15 Published:2011-12-13

摘要: 考察了活性炭对枯草芽孢杆菌β-甘露聚糖酶液的脱色与纯化作用。结果表明,酶液在脱色温度50℃、活性炭量3%、吸附时间2h条件下,不仅可使酶液脱去一部分色素,并纯化酶3.77倍,收率达到86.19%±2.99%。

关键词: 活性炭, &beta, -甘露聚糖酶, 纯化, 脱色

Abstract: The effect of active carbon on theβ- mannanase of Bacillus subtilis was investigated. The results showed that carbonwas found to be very effective for decolorizing crude beta-mannanase. The conditions of using 3% carbon, treated at 50℃ andpH 6.5 for a duration of 2h were optimal for decolorizing and purifying the enzyme. Under these conditions, the enzyme couldbe purified by 3.77±0.15 fold with a yielding recovery of 86.19%±2.99%.

Key words: active carbon, β- mannanase, purification, decolorizing