橙皮甙等桔皮活性成分的提取和抑菌作用研究

食品科学 ›› 2004, Vol. 25 ›› Issue (12): 112-115.

橙皮甙等桔皮活性成分的提取和抑菌作用研究

马庆一, 陈春涛, 荆晓艳, 袁卫霞

郑州轻工业学院食品与生物工程系

出版日期:2005-12-15 发布日期:2011-10-24

Studies on Extraction of Hesperidin and Other Active Components from Citrus Peels and Their Antimicrobial Effects

MA Qing-Yi, CHEN Chun-Tao, JING Xiao-Yan, YUAN Wei-Xia

Deptartment of Food and Biological Engineering,Zhengzhou Institute of Light Industry

Online:2005-12-15 Published:2011-10-24

摘要/Abstract

摘要： 本实验从桔皮中提取了数种色素和橙皮甙,再将后者酸解得到桔皮素。其抑菌性能测定确认了醇-水溶色素P2及醇溶色素P4对大肠杆菌、葡萄球菌,醚溶色素P1对青霉的活性。橙皮甙在pH4,浓度为2mg/ml时对大肠杆菌、葡萄球菌、枯草杆菌、青霉、黑曲霉、根霉和毛霉有显著抑制作用,而桔皮素仅对大肠杆菌有微弱活性。此结果揭示了桔皮抑菌防腐的主要有效成分是醇溶性色素以及橙皮甙;抗霉活性主要由醚溶色素负责,但在pH4,橙皮甙在酸协同下将发挥主要抑霉作用,而水解脱去糖甙后活性全无。

关键词: 桔皮色素, 橙皮甙, 桔皮素, 抑菌活性

Abstract: In this experiment several pigments and hesperidin were extracted from citrus peels, then hesperetin was obtained from hesperidin through acidic hydrolysis. The following activities were confirmed by antimicrobial effect assay:P2, P4 against Es.coli, Sta. Aureus;P1 against Penicllium;hesperidin (2mg/ml, pH4)against Es.coli, Sta. Aureus, Bacillus subtilis,Penicllium, Asper. Niger, Rhizopus and Mucors; hesperetin against Es.coli(weaker). The results revealed that the major antibacterial active components were alcohol-soluble pigments and hesperidin, however, the main antifungal ingredient was petroether-soluble pigment. Only at pH4, the hesperedin with synergistic acid became the major mould-inhibitor, but the hydrolyzed product, hesperitin had not any anti-mould effect.

Key words: citrus peels pigments, hesperidin, hesperitin, antimicrobial activity

马庆一, 陈春涛, 荆晓艳, 袁卫霞. 橙皮甙等桔皮活性成分的提取和抑菌作用研究[J]. 食品科学, 2004, 25(12): 112-115.

MA Qing-Yi, CHEN Chun-Tao, JING Xiao-Yan, YUAN Wei-Xia. Studies on Extraction of Hesperidin and Other Active Components from Citrus Peels and Their Antimicrobial Effects[J]. FOOD SCIENCE, 2004, 25(12): 112-115.

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