食品科学 ›› 2004, Vol. 25 ›› Issue (2): 114-117.

• 工艺技术 • 上一篇    下一篇

苦瓜皂甙快速分离纯化方法研究

 陈勋, 于海宁, 唐德松, 洪军, 沈生荣   

  1. 浙江大学农业与生物技术学院
  • 出版日期:2004-02-15 发布日期:2011-10-24

Assay Study on Rapid Separation and Purification of Saponin Extract from Momordica charantia L.

 CHEN  Xun, YU  Hai-Ning, TANG  De-Song, HONG  Jun, SHEN  Sheng-Rong   

  1. College of Agriculture and Bilology Technology, Zhejiang University
  • Online:2004-02-15 Published:2011-10-24

摘要: 用乙醇浸提葫芦科植物苦瓜(MomordicacharantiaL.),通过改变浸提液浓度,浸提时间和洗脱液浓度等,确定一种提取苦瓜总皂甙的优化工艺。用人参皂甙Rg1为对照品,通过选择最大吸收波长,绘制标准曲线定量测定苦瓜皂甙总量。

关键词: 苦瓜, 皂甙, 分离纯化

Abstract: The rapid routine assay of separation and purification on the extraction of saponin by alcohol from Momordicacharantia L. was studied in this paper. The solvent concentration, extraction period and the ratio of elution solution were consideredto ensure an optimized technological process to extract and isolate saponin from Momordica charantia L. Rg1(from Ginseng) wastaken as control.From the maxmium absorbance wavelength selected between the two UV spectra of saponin from Momordicacharantia L. and Rg1, a standard curve of Rg1 was made to determine the total content of saponin in the extracted products.

Key words: Momordica charantia L., saponin, separation and purification