食品科学 ›› 2004, Vol. 25 ›› Issue (4): 141-144.

• 分析检测 • 上一篇    下一篇

大豆异黄酮双向纸层析分析方法的研究

 彭义交, 刘宗林   

  1. 北京市食品研究所
  • 出版日期:2004-04-15 发布日期:2011-10-24

Study on Two-dimensional Paper-chromatography of Soybean Isoflavone

 PENG  Yi-Jiao, LIU  Zong-Lin   

  1. Beijing Food Research Institute
  • Online:2004-04-15 Published:2011-10-24

摘要: 大豆异黄酮是由大豆素、染料木素及其相应的衍生物组成。它们的化学性质极为相似,常见的分析方法多采用高压液相色谱法(HPLC),但由于液相色谱仪价格昂贵、操作复杂,故很难普及。我们采用双相纸层析方法其设备简单、操作方便,分离结果与液-质色谱法(LC-MS)基本吻合。

关键词: 大豆异黄酮, 大豆素, 染料木素, 纸层析

Abstract: Soybean isoflavone in made up of Daidzein, Genistein and its’ remification, they have the similar chemistry characters,the analytical method is HPLC, but the instrument is expensive, it can’t be popularized. We adopt the two-dimensional paper-chromatography, it is a kind of the convenient and very simple method. The result is same as the method LC-MS.

Key words: soybean isoflavone, daidzein, genistein, paper-chromatography