食品科学 ›› 2013, Vol. 34 ›› Issue (10): 112-116.doi: 10.7506/spkx1002-6630-201310024

• 工艺技术 • 上一篇    下一篇

响应面化优化豆酱中染料木素的乙醇-超声波法提取工艺

杨宏志,丁 洁,李志江,佟万兵   

  1. 黑龙江八一农垦大学食品学院,黑龙江 大庆 163319
  • 收稿日期:2012-02-13 修回日期:2013-04-03 出版日期:2013-05-25 发布日期:2013-05-07
  • 通讯作者: 丁洁 E-mail:20051063dingjie@163.com

Optimization of Ultrasonic-Assisted Ethanol Extraction of Genistein from Soybean Paste by Response Surface Methdology

YANG Hong-zhi,DING Jie,LI Zhi-jiang,TONG Wan-bing   

  1. College of Food Science, Heilongjiang Bayi Agricultural University, Daqing 163319, China
  • Received:2012-02-13 Revised:2013-04-03 Online:2013-05-25 Published:2013-05-07

摘要:

采用单因素试验和响应面分析法(RSM)研究乙醇体积分数、提取时间、提取温度对超声波提取染料木素效果的影响。以提取液中染料木素的提取量为响应值,通过响应面分析优化染料木素提取工艺条件。结果表明:影响染料木素提取效果的主要因素为乙醇体积分数,次要因素为提取时间和提取温度。染料木素适宜乙醇提取的工艺条件为乙醇体积分数78.97%、提取时间59.61min、提取温度49.40℃,提取功率弱,相应提取液中染料木素的为提取量100.07μg/g。

关键词: 豆酱, 超声波, 染料木素, 响应面分析

Abstract:

In this study, the ultrasonic-assisted ethanol extraction of genistein from soybean paste was optimized by
response surface methodology. The etraction efficiency of genistein was investigated with respect to ethanol concentration,
extraction time and extraction temperature. Ethanol concentration was found to be a main factor followed by extraction time
and temperature. The optimum conditions for extracting genistein from soybean paste were 78.97% ethanol, 59.61 min,
49.40 ℃ and low ultrasonic power. The resulting extract contained 100.07 μg/g.

Key words: soybean paste, ultrasonic, genistein, response surface analysis

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