关键词: 膨胀床, 藻蓝蛋白, 分离纯化, 偶联

Abstract:

Expanded bed enrichment coupled with impurity removal with a packed bed was used for the separation and
purification of phycocyanin. Crude phycocyanin extract was adsorbed by a self-made expanded bed (400 mm × 16 mm).
The resulting maximum purity (A620 nm/A280 nm) of phycocyanin was 2.02 compared to 0.23 for the crude extract. It was also
found that the phycocyanin concentration of the crude extract was 0.17 mg/mL and increased to 2.15 mg/mL after this
treatment, with a purity of 1.00 and a recovery of 59.45%. In the second step, a packed bed of XAD7HP macroporous resin
(200 mm × 10 mm) was used to remove impurity proteins from the crudely purified product. The maximum purity of
phycocyanin obtained under optimum conditions was 2.20 with a recovery of 58.81%, and the phycocyanin concentration of
the purfied product was 0.87 mg/mL. The recovery of phycocyanin from the initial crude extract was 34.96%.

Key words: expanded bed, phycocyanin, purification, coupling