UV、LiCl复合诱变深黄被孢霉选育多不饱和脂肪酸高产菌株

食品科学 ›› 2004, Vol. 25 ›› Issue (4): 50-52.

UV、LiCl复合诱变深黄被孢霉选育多不饱和脂肪酸高产菌株

王啸, 邱树毅, 何腊平

贵州工业大学贵州省发酵工程与生物制药重点实验室

出版日期:2004-04-15 发布日期:2011-10-24

Screening of a High Polyunsaturated Fatty Acid Yield with Mortierellaisabellina Mutated by UV and LiCl

WANG Xiao, QIU Shu-Yi, HE La-Ping

Province Key Laboratory of Fermentation Engineering and Biological Pharmacy,Guizhou University of Technology

Online:2004-04-15 Published:2011-10-24

摘要/Abstract

摘要： 以深黄被孢霉(Mortierellaisabellina)AS3.2793为出发菌株,利用紫外线和LiCl复合诱变处理,反复多次,最终选育出一支突变株MUI0310。其摇瓶发酵8d时,生物量和总脂含量分别达21.80g/L,61.47%,在同等条件下比出发菌株分别提高了107.62%和5.50%,气相色谱分析其油脂组成,多不饱和脂肪酸含量为23.21%。连续传代多次,其产量性状无显著变化。发酵实验表明菌株MUI0310极有潜力改良成为工业化生产菌种。

关键词: 深黄被孢霉, 多不饱和脂肪酸, 诱变

Abstract: A mutant strain MUI0310 was successfully obtained when the original strain Mortierella isabellina AS3.2793 wastreated by UV and LiCl as mutating agent again and again .The biomass was up to 21.80g/L, which was 107.62% higher than thatof the original strain while the content of lipid was up to 61.47%, which was 5.50% higher than that in the original strainmycelium. Analysis by gas chromatograph indicated the content of PUFA in the lipid was 23.21% . After passing on many times,there were no distinct changes on the production. Fermentation test shows MUI0310 has a capability to be improved to industrialstrain.

Key words: Mortierella isabellina, polyunsaturated fatty acid, mutation

王啸, 邱树毅, 何腊平. UV、LiCl复合诱变深黄被孢霉选育多不饱和脂肪酸高产菌株[J]. 食品科学, 2004, 25(4): 50-52.

WANG Xiao, QIU Shu-Yi, HE La-Ping. Screening of a High Polyunsaturated Fatty Acid Yield with Mortierellaisabellina Mutated by UV and LiCl[J]. FOOD SCIENCE, 2004, 25(4): 50-52.

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