食品科学 ›› 2004, Vol. 25 ›› Issue (4): 53-55.

• 基础研究 • 上一篇    下一篇

乳链菌肽产生菌的选育及其发酵性能研究

 罗水忠, 潘利华, 潘丽军   

  1. 合肥工业大学生物与食品工程学院
  • 出版日期:2004-04-15 发布日期:2011-10-24

Studies on Screening and Fermentation Characteristic by Streptococcus lactis

 LUO  Shui-Zhong, PAN  Li-Hua, PAN  Li-Jun   

  1. College of Biology and Food Engineering, Hefei University of Technology
  • Online:2004-04-15 Published:2011-10-24

摘要: 乳链菌肽是某些乳链球菌产生的一种多肽物质,对引起食品腐败的大多数革兰氏阳性菌有很好的抑制作用,是一种高效、无毒副作用的天然生物防腐剂。本文从鲜奶中筛选到一株乳链菌肽产生菌——乳链球菌P-99,并研究了该菌株的发酵适宜条件及其发酵过程动力学。结果表明,该菌株在M17培养基、30℃、起始pH6.5条件下发酵良好,通气量和某些金属离子对其细胞生长与产乳链菌肽无明显影响,但锰离子对乳链菌肽的产生有促进作用,而铜离子有较强的抑制作用;发酵动力学分析表明,该菌株产乳链菌肽表现出初级代谢动力学特征。

关键词: 乳链球菌, 乳链菌肽, 动力学方程

Abstract: Nisin, an effective, harmless and natural bio-preservative, is a kind of polypeptide produced by Streptococcus lactisand can protect the food being spoiled by G+ bacteria. A strain named Streptococcus lactis P-99 was isolated from fresh milk andits fermentation characteristic was studied. It was found that the optimal pH and temperature for cell growth and Nisin productionin M17 medium were 6.5 and 30 ℃ respectively, and that nisin production was increased by Mn2+ and inhibited strongly by Cu2+.The kinetics of cell growth and nisin production during culture of the strain was also explored.

Key words: Streptococcus lactis, Nisin, kinetic equation