食品科学 ›› 2005, Vol. 26 ›› Issue (1): 223-225.

• 营养卫生 • 上一篇    下一篇

苦瓜中植物胰岛素的分离及其降糖作用研究

 盛清凯, 姚惠源   

  1. 江南大学食品学院; 江南大学食品学院
  • 出版日期:2005-01-15 发布日期:2011-09-19

Isolation and Function of Plant Insulin Isolated from Momordica charantia

 SHENG  Qing-Kai, YAO  Hui-Yuan   

  1. School of Food Science and Technology,Southern Yangtze University
  • Online:2005-01-15 Published:2011-09-19

摘要: 改进苦瓜植物胰岛素的分离方法并探讨其降糖功能。采用有机酸、醇提取,SephadexG-50、反相高效液相色谱技术纯化,从苦瓜中分离出植物胰岛素。该技术优于过去的薄层层析纯化方法。皮下注射植物胰岛素可降低正常小鼠和四氧嘧啶模型小鼠的血糖。

关键词: 苦瓜, 植物胰岛素, 分离, 糖尿病

Abstract:  Isolation method of plant insulin from Momordica charantia was improved and its hypoglycemic function was probed. Plant insulin was extracted with organic acid and ethanol and purified with Sephadex G-50 gel filtration and reversed-phase high performance liquid chromatography (RP-HPLC) from Momordica charantia. The method was better than thin-layer chromatogra- phy technology. The peptide injected subcutaneously could lower blood sugar of normal mice and alloxan-induced diabetic mice.

Key words: Momordica charantia, plant insulin, isolation, diabetes