食品科学 ›› 2005, Vol. 26 ›› Issue (1): 264-270.

• 专题论述 • 上一篇    下一篇

反相C30柱在HPLC分析类胡萝卜素中的应用

 惠伯棣, 张西, 文镜   

  1. 北京联合大学生物活性物质与功能食品北京市重点实验室; 北京联合大学生物活性物质与功能食品北京市重点实验室
  • 出版日期:2005-01-15 发布日期:2011-09-19

Reverse-phase C30 HPLC Used in Analysis of Carotenoids

 HUI  Bo-Di, ZHANG  Xi, WEN  Jing   

  1. Beijing Laboratory of Bioactive Substances and Functional Foods
  • Online:2005-01-15 Published:2011-09-19

摘要: 本文介绍了一种最近在类胡萝卜素高压液相色谱分析中非常引人注目的固定相—反相C30柱,并例举了这种柱子在食品—特别在植物食品—的类胡萝卜素分析中的一些应用实例。与反相C18固定相相比,C30固定相分离类胡萝卜素的几何异构体具有明显的优势。因此,可以认为:应用反相C30柱分析生物样品—特别是食品材料—中的类胡萝卜素会具有良好的前景。

关键词: 反相C30柱, 高效液相色谱, 类胡萝卜素, 食品分析

Abstract: Reversed-phased C30 column, a currently favored stationary phase used in the HPLC separation and analysis of carotenoids was introduced herewith. Some working examples of this column on the separation of carotenoids from foodstuffs, especially plant food samples, were also reviewed. In comparison of reversed-phase C18, this stationary phase exhibited a greater benefit for the separation of geometrical (E/Z-) isomers and was therefore considered to have a bright future for the analysis of carotenoids from biological samples, especially from foodstuffs.

Key words: RP C30 column, HPLC, carotenoid, food analysis