食品科学 ›› 2005, Vol. 26 ›› Issue (12): 68-70.

• 基础研究 • 上一篇    下一篇

银杏愈伤组织单细胞克隆的研究

 秦卫东, 高明侠, 苗敬芝, 吕兆启   

  1. 徐州工程学院食品工程系
  • 出版日期:2005-12-15 发布日期:2011-10-01

Studies on Single-cells Cloning of Ginkgo Callus

 QIN  Wei-Dong, GAO  Ming-Xia, MIAO  Jing-Zhi, LU Zhao-Qi   

  1. Department of Food Engineering, Xuzhou Institute of Technology
  • Online:2005-12-15 Published:2011-10-01

摘要: 利用银杏单细胞克隆系研究银杏单细胞生长稳定性与细胞均一性之间的关系。结果显示:培养基过滤杀菌是促进细胞生长的好方法,当悬浮培养愈伤组织3代和向培养基中加入0.5g/L的酪蛋白水解物,能显著增加植板率,单细胞克隆的植板率为27.04%。克隆系GB-11平均生长率为0.55gfw/gfw•d。

关键词: 银杏, 愈伤组织, 单细胞克隆, 植板率, 生长速率

Abstract: The relationship between stability of ginkgo single-cells and cells uniformity its was studied by cells-cloning series of ginkgo. The results indicated: sterilization of medium by filtration was the good method to promote cells growth. When suspended culture of the 3rd generation callus, adding 0.5gL hydrates of casein in medium might increase planting efficiency. The planting efficiency of single-cells cloned was 27.04%. The average growth rates of GB-11 cloning lines reached 0.55g fwg fw•d.

Key words: ginkgo, callus, single-cells clone, plating efficiency, growth rates