植物源食品DNA制备方法的比较研究

食品科学 ›› 2005, Vol. 26 ›› Issue (2): 29-32.

植物源食品DNA制备方法的比较研究

周建嫦, 杨明杰, 杨杏芬, 黄俊明, 凌文华, 黄琼, 李文立

广东省疾病预防与控制中心毒理实验所；中山大学公共卫生学院营养学系；广东省疾病预防与控制中心毒理实验所

出版日期:2005-02-15 发布日期:2011-09-19

DNA Extraction from Genetically Modified Foods by Modified CTAB Method or SDS Method

ZHOU Jian-Chang, YANG Ming-Jie, YANG Xing-Fen, HUANG Jun-Ming, LING Wen-Hua, HUANG Qiong, LI Wen-Li

1.Institute of Toxicology, Guangdong Center of Disease Control and Prevention; 2.Department of Nutritional Science, School of Public Health, Sun Yat-sen University

Online:2005-02-15 Published:2011-09-19

摘要/Abstract

摘要： 目的:探索可用作PCR方法检测和鉴定植物源转基因食品模板的DNA抽提方法。方法:分别用改良CTAB法、SDS法制备黄豆、玉米、土豆和番茄及其加工产品的DNA,PCR扩增叶绿体基因片段及黄豆、玉米的内参照基因lectin和zein10。结果:改良CTAB法所得DNA作为PCR扩增模板,叶绿体基因片段及lectin和zein10均呈阳性,SDS法所得DNA作为模板时,部分样品内参照基因lectin或zein10扩增阴性。结论:PCR方法检测转基因食品时,应用改良CTAB法制备DNA模板。

关键词: 转基因食品, DNA抽提, PCR

Abstract: Object: To explore the DNA extraction methods for preparing DNA suitable for PCR-based detection of genetically modified organisms in foods and animals feed. Methods: DNAs of soybean, maize, tomato, potato and the processed products from these materials were extracted by using modified CTAB method or SDS method, and then were used as templates for PCR amplifying trnL gene of chloroplast as well as lectin gene of soybean and zein 10 gene of maize. Results: trnL gene of chloroplast and lectin gene of soybean and zein 10 gene of maize were revealed positive by PCR when using DNAs extraction by modified CTAB method as templates, while lectin and zein 10 were revealed negative by PCR amplifying of several DNA samples prepared by SDS method. Conclusion: DNA extract by using modified CTAB methods was suitable for PCR-based detection of genetically modified organisms in foods and animals feed.

Key words: genetically modified foods, DNA extraction, PCR

周建嫦, 杨明杰, 杨杏芬, 黄俊明, 凌文华, 黄琼, 李文立. 植物源食品DNA制备方法的比较研究[J]. 食品科学, 2005, 26(2): 29-32.

ZHOU Jian-Chang, YANG Ming-Jie, YANG Xing-Fen, HUANG Jun-Ming, LING Wen-Hua, HUANG Qiong, LI Wen-Li. DNA Extraction from Genetically Modified Foods by Modified CTAB Method or SDS Method[J]. FOOD SCIENCE, 2005, 26(2): 29-32.

[1]	刘婷. 欧盟转基因食品审慎监管经验及对我国的启示[J]. 食品科学, 2020, 41(5): 282-289.
[2]	江东健，罗秀儿，何庆华. 基于噬菌体展示纳米抗体的绿色免疫PCR检测脱氧雪腐镰刀菌烯醇[J]. 食品科学, 2019, 40(8): 256-261.
[3]	张娜娜，刘洋，俞漪，赵渝，窦同海，徐琼，段文锋，翁史昱. 乳酸菌饮料中嗜酸乳杆菌的实时荧光定量PCR检测方法[J]. 食品科学, 2019, 40(8): 27-32.
[4]	冯华炜，闫平平，艾海新，李思嘉，麻丽丹，刘宏生. 食品中甲型肝炎病毒RNA提取方法的比较及应用[J]. 食品科学, 2019, 40(6): 325-330.
[5]	李树，张伟，赵春梅. 易错PCR技术定向进化褐藻胶裂解酶Alg-2的分析[J]. 食品科学, 2019, 40(4): 146-151.
[6]	郭楠楠，张岩，李永波，张涛，张雅伦，周巍，王红. 微滴式数字PCR定量检测杏仁露中杏仁、花生源性成分[J]. 食品科学, 2019, 40(14): 346-351.
[7]	郭桂环，纪金言. 美国转基因食品强制标签立法及对我国的启示[J]. 食品科学, 2018, 39(9): 305-309.
[8]	冯可，胡文忠，姜爱丽，萨仁高娃，徐永平，司琦，马新秀. 鲜切果蔬中4 种病原微生物多重PCR检测技术[J]. 食品科学, 2018, 39(6): 276-283.
[9]	刘津，李婷，冼钰茵，吴希阳，凌莉，高东微. 转基因大豆MON89788双重数字PCR通用定量检测方法的建立[J]. 食品科学, 2018, 39(4): 312-319.
[10]	许随根，陈曦，李家鹏，李金春，杨君娜，熊苏玥，戚彪，米瑞芳，黄鑫，乔晓玲，王守伟. 应用多重PCR快速鉴定自然发酵肉制品中6 种葡萄球菌[J]. 食品科学, 2018, 39(24): 303-310.
[11]	蒋培基，王德良，徐东东，黄承雪，郭刚刚，栾春光，蒲彪. 基于竞争性等位基因特异性PCR技术的麦芽品种纯度定性和定量检测[J]. 食品科学, 2018, 39(24): 322-326.
[12]	胡安妥，王娉，张彩霞，蔡阳，陈颖. 5 类致泻性大肠埃希氏菌多重荧光PCR检测方法的建立[J]. 食品科学, 2018, 39(22): 249-255.
[13]	李葱葱，闫伟，夏蔚，董立明，龙丽坤，李飞武. 应用简并PCR方法检测转cry1A基因作物[J]. 食品科学, 2018, 39(14): 317-322.
[14]	赵秀丽，张毓秀，孙月芹，侯丽华. 利用易错PCR构建酱油酿造用T酵母spt15突变库[J]. 食品科学, 2018, 39(14): 106-111.
[15]	王小强，袁军，韩锐郡，营思思. 通用多重不对称PCR结合寡核苷酸芯片检测7 种食源性致病菌[J]. 食品科学, 2017, 38(8): 290-295.