Effects of Cordyceps Polysaccharide on Micronucleus Formation in Vicia faba Root Tip Cells Caused by Mitomycin-c

食品科学 ›› 2006, Vol. 27 ›› Issue (1): 231-234.

Effects of Cordyceps Polysaccharide on Micronucleus Formation in Vicia faba Root Tip Cells Caused by Mitomycin-c

陈宏伟, 朱蕴兰, 王慧春, 樊美珍

安徽农业大学省微生物防治重点实验室；徐州工程学院食品工程系；徐州工程学院环境工程系

出版日期:2006-01-15 发布日期:2011-09-05

Effects of Cordyceps Polysaccharide on Micronucleus Formation in Vicia faba Root Tip Cells Caused by Mitomycin-c

Chen-Hong-Wei, ZHU Yun-Lan, WANG Hui-Chun, FAN Mei-Zhen

1.Anhui Provincial Key Laboratory for Microbial Control, Anhui Agricultural University; 2.Food Engineering Department, Xuzhou Institute of Technology; 3.Environment Engineering Department, Xuzhou Institute of Technology.

Online:2006-01-15 Published:2011-09-05

摘要/Abstract

摘要： 目的:研究虫草多糖对丝裂霉素诱发的蚕豆根尖细胞微核的影响。方法:采用蚕豆根尖细胞微核试验。结果:浓度分别为100、80、50μg/ml的虫草胞内、胞外多糖对浓度为0.5μg/ml丝裂霉素诱发的蚕豆根尖微核率与阳性对照组(0.5μg/ml丝裂霉素)均具有极显著差异(p<0.01),虫草胞内、外多糖对微核的最大抑制率分别为60.48%、62.20%;浓度为30μg/ml的虫草胞内、外多糖对浓度为0.5μg/ml丝裂霉素诱发的蚕豆根尖微核率与阳性对照组(0.5μg/ml丝裂霉素)有显著的差异(p<0.05);四种浓度的胞内、外多糖之间对微核的影响至少存在显著的差异(p<0.05),且蚕豆根尖细胞微核率随多糖浓度的提高而降低。结论:虫草胞内、胞外多糖均可有效抑制丝裂霉素诱导的蚕豆根尖细胞微核的产生。

关键词: 虫草多糖, 蚕豆, 微核, 丝裂霉素

Abstract: Aim:To study the effects of cordyceps polysaccharide on micronucleus of Vicia faba root tip cells caused by mitomycin-c(MMC). Methods: Micronucleus test of Vicia faba root tip cells. Results: Vicia faba micronucleus rates(MCN‰) of cordyceps intracelluar polysaccharide and exopolysaccharide (The concentration was 100, 80, 50μg/ml respectively)caused by MMC(The concentration was 0.5μg/ml) were all less obviously than the positive control group (0.5μg/ml MMC; p <0.01); The maximal inhibition rates of cordyceps intracelluar polysaccharide and exopolysaccharide were 60.48%, 62.20% respectively. Vicia faba MCN‰ of cordyceps intracelluar polysaccharide and exopolysaccharide (The concentration was 30μg/ml) caused by MMC (The concentration was 0.5μg/ml) were significantly different in comparison with the positive control group(0.5μg/ml MMC; p<0.05). There was significant difference among the four concentrations of polysaccharide to the effects on micronucleus at least(p<0.05). In addition, with the increase of the concentration of polysaccharide Vicia faba incressed, the MCN‰ decreased. Conclusions: Cordyceps intracelluar polysaccharide and exopolysaccharide could inhibit the yield of micronucleus of Vicia faba root tip cells caused by MMC.

Key words: cordyceps polysaccharide, Vicia faba, micronucleus, mitomycin-c

陈宏伟, 朱蕴兰, 王慧春, 樊美珍. Effects of Cordyceps Polysaccharide on Micronucleus Formation in Vicia faba Root Tip Cells Caused by Mitomycin-c[J]. 食品科学, 2006, 27(1): 231-234.

Chen-Hong-Wei, ZHU Yun-Lan, WANG Hui-Chun, FAN Mei-Zhen. Effects of Cordyceps Polysaccharide on Micronucleus Formation in Vicia faba Root Tip Cells Caused by Mitomycin-c[J]. FOOD SCIENCE, 2006, 27(1): 231-234.

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Effects of Cordyceps Polysaccharide on Micronucleus Formation in Vicia faba Root Tip Cells Caused by Mitomycin-c

Effects of Cordyceps Polysaccharide on Micronucleus Formation in Vicia faba Root Tip Cells Caused by Mitomycin-c

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